GEN_DROPS
ID GEN_DROPS Reviewed; 754 AA.
AC Q29FC1;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Flap endonuclease GEN;
DE EC=3.1.-.-;
DE AltName: Full=Flap structure-specific endonuclease GEN;
DE AltName: Full=Xpg-like endonuclease;
GN Name=Gen; ORFNames=GA10481;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- FUNCTION: Endonuclease which cleaves flap structures at the junction
CC between single-stranded DNA and double-stranded DNA. Specific for 5'-
CC overhanging flap structures in which the 5'-upstream of the flap is
CC completely double-stranded. Prefers the blocked-flap structures similar
CC to those occurring at replication forks, in which the 5' single-strand
CC overhang of the flap is double-stranded. Also possesses weak 5'- to 3'-
CC exonuclease activity on nicked but not gapped double-stranded DNA. Does
CC not cleave bubble-like or Holliday junction substrates (By similarity).
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium ions per subunit. They probably participate in
CC the reaction catalyzed by the enzyme. May bind an additional third
CC magnesium ion after substrate binding. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.
CC {ECO:0000305}.
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DR EMBL; CH379067; EAL31334.1; -; Genomic_DNA.
DR RefSeq; XP_001354281.1; XM_001354245.3.
DR AlphaFoldDB; Q29FC1; -.
DR SMR; Q29FC1; -.
DR STRING; 7237.FBpp0276620; -.
DR EnsemblMetazoa; FBtr0278182; FBpp0276620; FBgn0070538.
DR GeneID; 4814122; -.
DR KEGG; dpo:Dpse_GA10481; -.
DR eggNOG; KOG2519; Eukaryota.
DR HOGENOM; CLU_013777_2_0_1; -.
DR InParanoid; Q29FC1; -.
DR OMA; MCAYLNE; -.
DR PhylomeDB; Q29FC1; -.
DR Proteomes; UP000001819; Chromosome X.
DR Bgee; FBgn0070538; Expressed in female reproductive system and 2 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:EnsemblMetazoa.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0035312; F:5'-3' exodeoxyribonuclease activity; ISS:UniProtKB.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IEA:EnsemblMetazoa.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:EnsemblMetazoa.
DR GO; GO:0008311; F:double-stranded DNA 3'-5' exodeoxyribonuclease activity; ISS:UniProtKB.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; ISS:UniProtKB.
DR GO; GO:0000400; F:four-way junction DNA binding; IEA:EnsemblMetazoa.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008310; F:single-stranded DNA 3'-5' exodeoxyribonuclease activity; ISS:UniProtKB.
DR GO; GO:0000737; P:DNA catabolic process, endonucleolytic; ISS:UniProtKB.
DR GO; GO:0000738; P:DNA catabolic process, exonucleolytic; ISS:UniProtKB.
DR GO; GO:0006302; P:double-strand break repair; IEA:EnsemblMetazoa.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR041012; GEN_chromo.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR006086; XPG-I_dom.
DR InterPro; IPR006084; XPG/Rad2.
DR InterPro; IPR006085; XPG_DNA_repair_N.
DR PANTHER; PTHR11081; PTHR11081; 1.
DR Pfam; PF18704; Chromo_2; 1.
DR Pfam; PF00867; XPG_I; 1.
DR Pfam; PF00752; XPG_N; 1.
DR PRINTS; PR00853; XPGRADSUPER.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00484; XPGI; 1.
DR SMART; SM00485; XPGN; 1.
DR SUPFAM; SSF47807; SSF47807; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; Endonuclease; Exonuclease; Hydrolase; Magnesium;
KW Metal-binding; Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..754
FT /note="Flap endonuclease GEN"
FT /id="PRO_0000314149"
FT REGION 1..90
FT /note="N-domain"
FT REGION 130..226
FT /note="I-domain"
FT REGION 479..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 732..754
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 479..496
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 737..754
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 30
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 74
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 142
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 144
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 163
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 165
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 222
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 754 AA; 85187 MW; A0D064A37CD3FAB9 CRC64;
MGVKELWTVL TPHAERKPIN ELRGKKVAID LAGWVCESLN VVDYFVHPRH HLKNLFFRTC
YLIWEQVTPV FVLEGVAPKL KGQVIAKRNE LQFRGVRPKD AATGTQTAAK VDKGRTRFNH
VLKQCETLLL SMGIQCVQGP GEAEAYAAFL NKHGLVDGVI SQDSDCFAYG AIRVYRNFSV
STQGAQAAAG GAVDIYDMRE ITSRMDFGQH KIIVMALLCG CDYCPDGIGG IGKDGVLKLF
NKYKESEILD RLRNWRAETD KYNALEMRVD DKSICSNCGH IGRTQSHTKS GCSVCRTKRG
CDKTLWKEQR LSIKAELILR RKALLAPEFP NEEIISEFLS EPPTIPNLNL GWRQPNLVKF
IKQIGHLLQW PEIYCFQKFF PILTRWQVQQ AARTNAIGRV ELVQPVDIIK KRTVKGVASL
ELRWQDPSGS FQGLIPDKQI SEFELEHPKG IEELYYTVEP LDMLEAAYPD LVASFLKSKE
KPPKKATRKK KTDPLSAIEN IPETLDKQKA NPAKPKRVVK KKKAPTEQAQ PSLQQFLRRE
KIGGTPVKDS LPQMAQLPQQ CSTPITKFLP SDLESDCDAV EFDMSDVVNG IISNPNARPT
VTRHEGRQLH YEALSDDLSM RLAQLSLRKD ELQEEPLPPV AEHKRDLSLV EHLPQSKRLS
LDDSFDLLVK GDLKKVPHLI QPIRTPVDRF KHQHRISEHL PQPAVEPAAN VSYFFNQSSD
NADAFEQLMN SSLGIQEQAE EDEEEEDDLV VISD
