ALPS_SCYSE
ID ALPS_SCYSE Reviewed; 123 AA.
AC B5TTX7;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 04-NOV-2008, sequence version 1.
DT 25-MAY-2022, entry version 20.
DE RecName: Full=Anti-lipopolysaccharide factor {ECO:0000303|PubMed:19490944, ECO:0000312|EMBL:ACH87655.1};
DE Short=SsALF {ECO:0000303|PubMed:19490944};
DE Flags: Precursor;
OS Scylla serrata (Mud crab).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Brachyura;
OC Eubrachyura; Portunoidea; Portunidae; Scylla.
OX NCBI_TaxID=6761;
RN [1] {ECO:0000305, ECO:0000312|EMBL:ACH87655.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RC TISSUE=Hemocyte {ECO:0000269|PubMed:19490944};
RX PubMed=19490944; DOI=10.1016/j.fsi.2009.05.009;
RA Yedery R.D., Reddy K.V.;
RT "Identification, cloning, characterization and recombinant expression of an
RT anti-lipopolysaccharide factor from the hemocytes of Indian mud crab,
RT Scylla serrata.";
RL Fish Shellfish Immunol. 27:275-284(2009).
RN [2] {ECO:0000305}
RP FUNCTION.
RX PubMed=21195157; DOI=10.1016/j.micpath.2010.12.007;
RA Sharma S., Yedery R.D., Patgaonkar M.S., Selvaakumar C., Reddy K.V.;
RT "Antibacterial activity of a synthetic peptide that mimics the LPS binding
RT domain of Indian mud crab, Scylla serrata anti-lipopolysaccharide factor
RT (SsALF) also involved in the modulation of vaginal immune functions through
RT NF-kB signaling.";
RL Microb. Pathog. 50:179-191(2011).
CC -!- FUNCTION: Binds to bacterial LPS and may specifically inhibit the LPS-
CC mediated activation of the hemolymph coagulation. It has a strong
CC antibacterial effect especially on the growth of Gram-negative
CC bacteria. {ECO:0000269|PubMed:19490944, ECO:0000269|PubMed:21195157}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Strong expression in hemocytes, heart and muscle,
CC with weaker expression detected in gills and hepatopancreas. No
CC expression detected in eyes. {ECO:0000269|PubMed:19490944}.
CC -!- INDUCTION: By injection with lipopolysaccharide. Expression is highest
CC at 3 hours post-injection, decreases to basal levels at 12 hours post-
CC injection and is up-regulated again at 24 hours post-injection. In the
CC presence of recombinant ALF, the expression is significantly down-
CC regulated at 3 hours and 6 hours post-injection.
CC {ECO:0000269|PubMed:19490944}.
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DR EMBL; FJ013272; ACH87655.1; -; mRNA.
DR PDB; 2MDL; NMR; -; A=54-77.
DR PDBsum; 2MDL; -.
DR AlphaFoldDB; B5TTX7; -.
DR BMRB; B5TTX7; -.
DR SMR; B5TTX7; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR Gene3D; 3.30.160.320; -; 1.
DR InterPro; IPR024509; Anti-LPS_factor/Scygonadin.
DR InterPro; IPR038539; Anti-LPS_factor/Scygonadin_sf.
DR Pfam; PF11630; DUF3254; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic; Antimicrobial; Disulfide bond; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255, ECO:0000312|EMBL:ACH87655.1"
FT CHAIN 27..123
FT /note="Anti-lipopolysaccharide factor"
FT /evidence="ECO:0000255, ECO:0000312|EMBL:ACH87655.1"
FT /id="PRO_5000399109"
FT DISULFID 55..76
FT /evidence="ECO:0000250|UniProtKB:P07086"
FT STRAND 65..68
FT /evidence="ECO:0007829|PDB:2MDL"
FT STRAND 70..73
FT /evidence="ECO:0007829|PDB:2MDL"
SQ SEQUENCE 123 AA; 14041 MW; 9EA3CED0FE8DE75C CRC64;
MRTRVMAGLC VALVVMCLYM PQPCEAQYEA LVASILGKLS GLWHSDTVDF MGHTCHIRRK
PKFRKFKLYH EGKFWCPGWT HLEGNSRTKS RSGSTREATK DFVHKALQNK LITKNSADAW
LKG
