GERAA_BACSU
ID GERAA_BACSU Reviewed; 482 AA.
AC P07868; O32195;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 3.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Spore germination protein A1;
GN Name=gerAA; Synonyms=gerA1; OrderedLocusNames=BSU33050;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=2998952; DOI=10.1016/0378-1119(85)90207-0;
RA Feavers I.M., Miles J.S., Moir A.;
RT "The nucleotide sequence of a spore germination gene (gerA) of Bacillus
RT subtilis 168.";
RL Gene 38:95-102(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9639930; DOI=10.1099/00221287-144-6-1593;
RA Wipat A., Brignell C.S., Guy J.B., Rose M., Emmerson P.T., Harwood C.R.;
RT "The yvsA-yvqA (293 degrees - 289 degrees) region of the Bacillus subtilis
RT chromosome containing genes involved in metal ion uptake and a putative
RT sigma factor.";
RL Microbiology 144:1593-1600(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 299 AND 302.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 466-482.
RC STRAIN=168;
RX PubMed=3110007; DOI=10.1016/0378-1119(87)90468-9;
RA Zuberi A.R., Moir A., Feavers I.M.;
RT "The nucleotide sequence and gene organization of the gerA spore
RT germination operon of Bacillus subtilis 168.";
RL Gene 51:1-11(1987).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
RC STRAIN=168;
RX PubMed=3923430; DOI=10.1093/nar/13.1.131;
RA Miles J.S., Guest J.R.;
RT "Complete nucleotide sequence of the fumarase gene (citG) of Bacillus
RT subtilis 168.";
RL Nucleic Acids Res. 13:131-140(1985).
CC -!- FUNCTION: Forms a complex at the inner spore membrane which acts as a
CC receptor for L-alanine, thus is involved in the stimulation of
CC germination in response to alanine. Can stimulate germination in the
CC absence of GerD and GerK gene products (fructose and glucose receptors,
CC respectively), but the response is improved in their presence.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the forespore compartment of the
CC developing sporangium.
CC -!- SIMILARITY: Belongs to the GerABKA family. {ECO:0000305}.
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DR EMBL; M11918; AAA22465.1; -; Genomic_DNA.
DR EMBL; AJ223978; CAA11748.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15295.2; -; Genomic_DNA.
DR EMBL; M16189; AAA92780.1; -; Genomic_DNA.
DR EMBL; X01701; CAA25848.1; -; Genomic_DNA.
DR PIR; E69629; E69629.
DR RefSeq; NP_391185.2; NC_000964.3.
DR RefSeq; WP_009968144.1; NZ_CP010052.1.
DR RefSeq; WP_010886610.1; NZ_CP053102.1.
DR AlphaFoldDB; P07868; -.
DR SMR; P07868; -.
DR STRING; 224308.BSU33050; -.
DR PaxDb; P07868; -.
DR EnsemblBacteria; CAB15295; CAB15295; BSU_33050.
DR GeneID; 935953; -.
DR KEGG; bsu:BSU33050; -.
DR PATRIC; fig|224308.43.peg.3462; -.
DR eggNOG; COG0697; Bacteria.
DR InParanoid; P07868; -.
DR OMA; FTVPQYG; -.
DR PhylomeDB; P07868; -.
DR BioCyc; BSUB:BSU33050-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009847; P:spore germination; IEA:InterPro.
DR InterPro; IPR004995; Spore_Ger.
DR Pfam; PF03323; GerA; 1.
DR PIRSF; PIRSF005690; GerBA; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Germination; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..482
FT /note="Spore germination protein A1"
FT /id="PRO_0000164014"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 299
FT /note="T -> A (in Ref. 1; AAA22465 and 2; CAA11748)"
FT /evidence="ECO:0000305"
FT CONFLICT 302
FT /note="S -> P (in Ref. 2; CAA11748)"
FT /evidence="ECO:0000305"
FT CONFLICT 347..348
FT /note="Missing (in Ref. 1; AAA22465)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 482 AA; 53810 MW; C89C775A69ACCBE5 CRC64;
MEQTEFKEYI HDNLALVLPK LKENDDLVKN KKMLANGLVF YYLYFSEMTD ENKVSEAIKT
LIKDEETLTL DQVKKRLDQL DARPVETAKK TIESILNGNC AVFINGLDKA YILTTGKKKT
RSLTEPTTEK VVRGPKVAFV EDIDTNLALI RQRTSHPKLI TKKIMIGENK LKPAAIMYIE
GKAKKSVIKE VKARLKNIQL EDIQDSGTLE ELIEDNKYSP FPQIQNTERP DKVSSALFNG
RVAILVDSSP FVLLVPVSLG ILMQSPDDYY ERWISASLIR SLRFASIFIT LFLSSIYITL
VSFHQGLLPT ALAVTISANR ENVPFPPIFE ALLMEVTIEL LREAGLRLPN PLGQTIGLVG
GVVIGQAAVE ANLVSSILVI VVSVIALASF TVPQYGMGLS FRVLRFISMF SAAILGLYGI
ILFMLVVYTH LTRQTSFGSP YFSPNGFFSL KNTDDSIIRL PIKNKPKEVN NPNEPKTDST
ET
