GERBC_BACSU
ID GERBC_BACSU Reviewed; 374 AA.
AC P39571;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Spore germination protein B3;
DE Flags: Precursor;
GN Name=gerBC; OrderedLocusNames=BSU35820;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8012571; DOI=10.1099/00221287-140-3-471;
RA Corfe B.M., Sammons R.L., Smith D.A., Maueel C.;
RT "The gerB region of the Bacillus subtilis 168 chromosome encodes a
RT homologue of the gerA spore germination operon.";
RL Microbiology 140:471-478(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Involved in the response to the germinative mixture of L-
CC asparagine, glucose, fructose and potassium ions (AGFK). Cannot
CC stimulate germination in the absence of gerD and gerK gene products
CC (fructose and glucose receptors respectively).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the forespore compartment of the
CC developing sporangium.
CC -!- SIMILARITY: Belongs to the GerABKC lipoprotein family. {ECO:0000305}.
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DR EMBL; L16960; AAA22468.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15599.1; -; Genomic_DNA.
DR PIR; I39857; I39857.
DR RefSeq; NP_391463.1; NC_000964.3.
DR RefSeq; WP_003242990.1; NZ_JNCM01000034.1.
DR PDB; 3N54; X-ray; 2.30 A; B=25-374.
DR PDBsum; 3N54; -.
DR AlphaFoldDB; P39571; -.
DR SMR; P39571; -.
DR STRING; 224308.BSU35820; -.
DR PaxDb; P39571; -.
DR PRIDE; P39571; -.
DR EnsemblBacteria; CAB15599; CAB15599; BSU_35820.
DR GeneID; 936819; -.
DR KEGG; bsu:BSU35820; -.
DR PATRIC; fig|224308.179.peg.3878; -.
DR eggNOG; ENOG502Z8GN; Bacteria.
DR OMA; GYNGKIM; -.
DR PhylomeDB; P39571; -.
DR BioCyc; BSUB:BSU35820-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009847; P:spore germination; IEA:InterPro.
DR Gene3D; 3.30.300.210; -; 1.
DR InterPro; IPR008844; Spore_GerAC.
DR InterPro; IPR038501; Spore_GerAC_C_sf.
DR PANTHER; PTHR35789; PTHR35789; 1.
DR Pfam; PF05504; Spore_GerAC; 1.
DR TIGRFAMs; TIGR02887; spore_ger_x_C; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Germination; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 20..374
FT /note="Spore germination protein B3"
FT /id="PRO_0000018174"
FT LIPID 20
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 20
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305"
FT STRAND 32..39
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 45..52
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 66..68
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 70..79
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 83..87
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 97..101
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 103..108
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 111..118
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 121..123
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 129..135
FT /evidence="ECO:0007829|PDB:3N54"
FT TURN 138..140
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 176..184
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 189..210
FT /evidence="ECO:0007829|PDB:3N54"
FT TURN 211..214
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 215..220
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 222..231
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 239..244
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 247..264
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 266..282
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 295..321
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 330..337
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 339..345
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 346..348
FT /evidence="ECO:0007829|PDB:3N54"
FT HELIX 349..355
FT /evidence="ECO:0007829|PDB:3N54"
FT STRAND 357..366
FT /evidence="ECO:0007829|PDB:3N54"
SQ SEQUENCE 374 AA; 42469 MW; 2E2CBE04E80E8A86 CRC64;
MKTASKFSVM FFMLLALCGC WDVKDIEQLS FARGLAIDET NDHQYKLTYQ NLLPQSEDSQ
ASGKPEFVNV TSHGKTILEA VSDVSIKDPP VYSDHLKVIL LGEKLMRNQN VDQVLNHFIR
DDELRRSSYL MAARGNAADV FTKGNPNQQQ PMPSEKLIDL TTHSGYNGKI MIPLRIGRAS
VYSQNGYSYL IQAVKNEKGK AKYDGAGIIK RGSNKLVGFL SADETQTLSW VMGTIQGGVM
PTTDKGHPIT FEIKKSKTKI KPVIENGKPV FHISVKTKGI LTEDQNPNEN SFSKSYLHRL
ENIFEKKLER DVKQVMDKLQ HEYKTDPVFL SDHIRIQHPD YWNKVKGHWD EIFSETDFKY
DISFKIINFG TVGK
