GERE_BACSU
ID GERE_BACSU Reviewed; 74 AA.
AC P11470;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Spore germination protein GerE;
GN Name=gerE; OrderedLocusNames=BSU28410;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3114423; DOI=10.1099/00221287-132-11-3013;
RA Cutting S.M., Mandelstam J.;
RT "The nucleotide sequence and the transcription during sporulation of the
RT gerE gene of Bacillus subtilis.";
RL J. Gen. Microbiol. 132:3013-3024(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969504; DOI=10.1099/13500872-142-11-3067;
RA Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J.,
RA Emmerson P.T., Harwood C.R.;
RT "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis
RT chromosome containing genes responsible for stress responses, the
RT utilization of plant cell walls and primary metabolism.";
RL Microbiology 142:3067-3078(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / IG20;
RA Ducros V., Brannigan J.A., Lewis R.J., Wilkinson A.J.;
RT "Crystallisation and preliminary X-ray diffraction analysis of the
RT sporulation regulatory protein GerE from Bacillus subtilis.";
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP SEQUENCE REVISION TO 11.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
RX PubMed=2474075; DOI=10.1016/0022-2836(89)90262-3;
RA Cutting S.M., Panzer S., Losick R.;
RT "Regulatory studies on the promoter for a gene governing synthesis and
RT assembly of the spore coat in Bacillus subtilis.";
RL J. Mol. Biol. 207:393-404(1989).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS).
RX PubMed=11243786; DOI=10.1006/jmbi.2001.4443;
RA Ducros V.M.-A., Lewis R.J., Verma C.S., Dodson E.J., Leonard G.,
RA Turkenburg J.P., Murshudov G.N., Wilkinson A.J., Brannigan J.A.;
RT "Crystal structure of GerE, the ultimate transcriptional regulator of spore
RT formation in Bacillus subtilis.";
RL J. Mol. Biol. 306:759-771(2001).
CC -!- FUNCTION: Involved in the regulation of spore formation. Directs the
CC transcription of several genes that encode structural components of the
CC protein coat that encases the mature spore (CotB, CotC, CotG, CotS,
CC CotV, CotW, CotX, CotY and CotZ). Controls also the cgeAB and cgeCDE
CC operons.
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DR EMBL; M17642; AAA22473.1; -; Genomic_DNA.
DR EMBL; Z75208; CAA99550.1; -; Genomic_DNA.
DR EMBL; AJ223964; CAA11701.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14801.2; -; Genomic_DNA.
DR PIR; C27893; C27893.
DR RefSeq; NP_390719.2; NC_000964.3.
DR RefSeq; WP_003184172.1; NZ_JNCM01000036.1.
DR PDB; 1FSE; X-ray; 2.05 A; A/B/C/D/E/F=1-74.
DR PDBsum; 1FSE; -.
DR AlphaFoldDB; P11470; -.
DR SMR; P11470; -.
DR STRING; 224308.BSU28410; -.
DR PaxDb; P11470; -.
DR PRIDE; P11470; -.
DR EnsemblBacteria; CAB14801; CAB14801; BSU_28410.
DR GeneID; 61769551; -.
DR GeneID; 64304567; -.
DR GeneID; 66364067; -.
DR GeneID; 937462; -.
DR KEGG; bsu:BSU28410; -.
DR PATRIC; fig|224308.179.peg.3086; -.
DR eggNOG; COG2197; Bacteria.
DR InParanoid; P11470; -.
DR OMA; HISNTMQ; -.
DR PhylomeDB; P11470; -.
DR BioCyc; BSUB:BSU28410-MON; -.
DR EvolutionaryTrace; P11470; -.
DR PRO; PR:P11470; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00196; GerE; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR PROSITE; PS00622; HTH_LUXR_1; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Germination; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..74
FT /note="Spore germination protein GerE"
FT /id="PRO_0000184159"
FT DOMAIN 5..70
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 29..48
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT CONFLICT 11
FT /note="L -> S (in Ref. 1; AAA22473, 2; CAA99550 and 6)"
FT /evidence="ECO:0000305"
FT HELIX 14..23
FT /evidence="ECO:0007829|PDB:1FSE"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:1FSE"
FT HELIX 29..36
FT /evidence="ECO:0007829|PDB:1FSE"
FT HELIX 40..54
FT /evidence="ECO:0007829|PDB:1FSE"
FT HELIX 59..68
FT /evidence="ECO:0007829|PDB:1FSE"
SQ SEQUENCE 74 AA; 8588 MW; 8EF9C6194D39556B CRC64;
MKEKEFQSKP LLTKREREVF ELLVQDKTTK EIASELFISE KTVRNHISNA MQKLGVKGRS
QAVVELLRMG ELEL
