GERKA_BACSU
ID GERKA_BACSU Reviewed; 544 AA.
AC P49939; P94410;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Spore germination protein KA;
GN Name=gerKA; OrderedLocusNames=BSU03700;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Irie R., Fujita Y.Y.F., Kobayasi M.;
RT "Nucleotide sequence and gene organization of the gerK spore germinating
RT locus of Bacillus subtilis 168.";
RL Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA Yamane K., Kumano M., Kurita K.;
RT "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT determination of the sequence of a 146 kb segment and identification of 113
RT genes.";
RL Microbiology 142:3047-3056(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Involved in the germination response to the combination of
CC glucose, fructose, L-asparagine, and KCl.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the GerABKA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA11254.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D78187; BAA11254.1; ALT_INIT; Genomic_DNA.
DR EMBL; D50453; BAA09002.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12178.1; -; Genomic_DNA.
DR PIR; I39858; I39858.
DR RefSeq; NP_388252.1; NC_000964.3.
DR RefSeq; WP_003246515.1; NZ_JNCM01000031.1.
DR AlphaFoldDB; P49939; -.
DR SMR; P49939; -.
DR STRING; 224308.BSU03700; -.
DR PaxDb; P49939; -.
DR PRIDE; P49939; -.
DR EnsemblBacteria; CAB12178; CAB12178; BSU_03700.
DR GeneID; 938285; -.
DR KEGG; bsu:BSU03700; -.
DR PATRIC; fig|224308.179.peg.390; -.
DR eggNOG; COG0697; Bacteria.
DR InParanoid; P49939; -.
DR OMA; SHCITEE; -.
DR PhylomeDB; P49939; -.
DR BioCyc; BSUB:BSU03700-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009847; P:spore germination; IEA:InterPro.
DR InterPro; IPR004995; Spore_Ger.
DR Pfam; PF03323; GerA; 1.
DR PIRSF; PIRSF005690; GerBA; 1.
PE 3: Inferred from homology;
KW Cell membrane; Germination; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..544
FT /note="Spore germination protein KA"
FT /id="PRO_0000164016"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 443..463
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 504..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..29
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 504..523
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 2
FT /note="P -> R (in Ref. 1; BAA11254)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="Q -> E (in Ref. 1; BAA11254)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="E -> R (in Ref. 1; BAA11254)"
FT /evidence="ECO:0000305"
FT CONFLICT 114
FT /note="L -> A (in Ref. 1; BAA11254)"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="E -> G (in Ref. 1; BAA11254)"
FT /evidence="ECO:0000305"
FT CONFLICT 202
FT /note="K -> E (in Ref. 1; BAA11254)"
FT /evidence="ECO:0000305"
FT CONFLICT 376
FT /note="E -> K (in Ref. 1; BAA11254)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 544 AA; 60386 MW; 131CFBBF45EF882A CRC64;
MPLFSKRKNN TDSKDKQNTD ERNQEQQQEK ERPVLISPSL AKNIAETKKE VGSSSDVIIR
EIKIGEQDHV HLAVIYISGL VDNNTIHESL IDPLVQDESI QNTHAIQQIL EKTLPLGGVK
AEKSWDKLFS ELMLGNALIF ADGHDEALIC STQGGEQRSI QEPSTQVSFR GPRQGFTESL
QTNISMIRRY IKNPNLWVEK MKKGSVTNTD IALMYIQGIC DEKVLKEVKQ RLEKIDIDSI
LESGYIEQLI EDETFTTFPT MYHTERPDVV AGNLLEGRFA IIVDGTPFVL IAPALFVQFF
QSVEDYYSRF DIATSIRILR VLVFFISLVA PAVYVAATTF HQEMIPTQLL VVIAAQREIV
PFPAVVEALT MEVAFEILRE AGVRLPRVVG SAVSIVGALV IGQAAVQAGI VSPAMVIIVA
LTAIASFATP AFAMAISARL IRFIFIIASA VMGFYGLILG IIMMFVHLCS LRSFGVPYMS
PLAPFSSQGV KDALFRVPWW ADEKRPESVS KEDKVRQGKD QRPEPAASRG MVNKDLEEGD
QNGT
