ALR1_CALS4
ID ALR1_CALS4 Reviewed; 388 AA.
AC Q8RAK6;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Alanine racemase 1 {ECO:0000255|HAMAP-Rule:MF_01201};
DE EC=5.1.1.1 {ECO:0000255|HAMAP-Rule:MF_01201};
GN Name=alr1; OrderedLocusNames=TTE1207;
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4;
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
CC -!- FUNCTION: Catalyzes the interconversion of L-alanine and D-alanine. May
CC also act on other amino acids. {ECO:0000255|HAMAP-Rule:MF_01201}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-alanine = D-alanine; Xref=Rhea:RHEA:20249,
CC ChEBI:CHEBI:57416, ChEBI:CHEBI:57972; EC=5.1.1.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01201};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01201};
CC -!- PATHWAY: Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine
CC from L-alanine: step 1/1. {ECO:0000255|HAMAP-Rule:MF_01201}.
CC -!- SIMILARITY: Belongs to the alanine racemase family. {ECO:0000255|HAMAP-
CC Rule:MF_01201}.
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DR EMBL; AE008691; AAM24437.1; -; Genomic_DNA.
DR RefSeq; WP_011025537.1; NC_003869.1.
DR PDB; 4Y2W; X-ray; 2.70 A; A/B=1-388.
DR PDBsum; 4Y2W; -.
DR AlphaFoldDB; Q8RAK6; -.
DR SMR; Q8RAK6; -.
DR STRING; 273068.TTE1207; -.
DR PRIDE; Q8RAK6; -.
DR EnsemblBacteria; AAM24437; AAM24437; TTE1207.
DR KEGG; tte:TTE1207; -.
DR eggNOG; COG0787; Bacteria.
DR HOGENOM; CLU_028393_2_2_9; -.
DR OMA; ELMAVQH; -.
DR OrthoDB; 859043at2; -.
DR BRENDA; 5.1.1.1; 6784.
DR UniPathway; UPA00042; UER00497.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0008784; F:alanine racemase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:UniProtKB-UniRule.
DR GO; GO:0030632; P:D-alanine biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.40.37.10; -; 1.
DR Gene3D; 3.20.20.10; -; 1.
DR HAMAP; MF_01201; Ala_racemase; 1.
DR InterPro; IPR000821; Ala_racemase.
DR InterPro; IPR009006; Ala_racemase/Decarboxylase_C.
DR InterPro; IPR011079; Ala_racemase_C.
DR InterPro; IPR001608; Ala_racemase_N.
DR InterPro; IPR020622; Ala_racemase_pyridoxalP-BS.
DR InterPro; IPR029066; PLP-binding_barrel.
DR Pfam; PF00842; Ala_racemase_C; 1.
DR Pfam; PF01168; Ala_racemase_N; 1.
DR PRINTS; PR00992; ALARACEMASE.
DR SMART; SM01005; Ala_racemase_C; 1.
DR SUPFAM; SSF50621; SSF50621; 1.
DR SUPFAM; SSF51419; SSF51419; 1.
DR TIGRFAMs; TIGR00492; alr; 1.
DR PROSITE; PS00395; ALANINE_RACEMASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isomerase; Pyridoxal phosphate; Reference proteome.
FT CHAIN 1..388
FT /note="Alanine racemase 1"
FT /id="PRO_0000114588"
FT ACT_SITE 40
FT /note="Proton acceptor; specific for D-alanine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
FT ACT_SITE 268
FT /note="Proton acceptor; specific for L-alanine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
FT BINDING 138
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
FT BINDING 316
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
FT MOD_RES 40
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01201"
FT STRAND 8..14
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 15..28
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 34..38
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 40..45
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 48..58
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 61..67
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 68..76
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 83..85
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 91..93
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 94..99
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 103..106
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 109..121
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 126..132
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 134..136
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 138..142
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 144..155
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 160..166
FT /evidence="ECO:0007829|PDB:4Y2W"
FT TURN 172..175
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 177..195
FT /evidence="ECO:0007829|PDB:4Y2W"
FT TURN 196..198
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 202..208
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 209..214
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 216..219
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 220..224
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 227..230
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 236..238
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 248..253
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 256..260
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 265..267
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 268..270
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 278..284
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 287..289
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 293..295
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 300..303
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 306..312
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 319..322
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 334..340
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 348..354
FT /evidence="ECO:0007829|PDB:4Y2W"
FT HELIX 359..365
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 372..376
FT /evidence="ECO:0007829|PDB:4Y2W"
FT STRAND 379..384
FT /evidence="ECO:0007829|PDB:4Y2W"
SQ SEQUENCE 388 AA; 44126 MW; 8D8E10CF5E20DC9E CRC64;
MKFDGVRPTR VEVYLDAITH NFREIKKIVG KNVKIMAVIK GDAYGHGASY VAKFLEKEGV
DYFGVATTEE ALELREKGIK TPILIFGYTP PTQLRQIVKH DLTQTVYDIK YAKELEKESL
KQNKRAKVHI KIDTGLGRIG YIDFDLAQKE ILEMANMRGL ILEGIYSHFA AASEDDRDYC
KEQFDKFMNL ISSLEKKRLK IPLKHIANAA AILNLNYSHL DMVRPGIILF GAYPSKRVER
KVELRETLRF TTRVVHLKDV PAGFFIGYGK SFVTKRKSVI ATIPVGYADG LDRRLSNNYK
LLLKGKYVPI VGRVCMDQCM IDVTDVEGVE IGDEVVIIGT QNNETVSVES MADKIETIPQ
EVFSRISRRV PRVYFYDGIK IGEVNYLK
