ID   GERXA_BACAN             Reviewed;         492 AA.
AC   Q9ZFB4;
DT   24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Spore germination protein XA;
GN   Name=gerXA; OrderedLocusNames=pXO1-113, BXA0157, GBAA_pXO1_0157;
OS   Bacillus anthracis.
OG   Plasmid pXO1.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Sterne;
RX   PubMed=10411756; DOI=10.1046/j.1365-2958.1999.01485.x;
RA   Guidi-Rontani C., Pereira Y., Ruffie S., Sirard J.-C., Weber-Levy M.,
RA   Mock M.;
RT   "Identification and characterization of a germination operon on the
RT   virulence plasmid pXO1 of Bacillus anthracis.";
RL   Mol. Microbiol. 33:407-414(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RX   PubMed=10515943; DOI=10.1128/jb.181.20.6509-6515.1999;
RA   Okinaka R.T., Cloud K., Hampton O., Hoffmaster A.R., Hill K.K., Keim P.,
RA   Koehler T.M., Lamke G., Kumano S., Mahillon J., Manter D., Martinez Y.,
RA   Ricke D., Svensson R., Jackson P.J.;
RT   "Sequence and organization of pXO1, the large Bacillus anthracis plasmid
RT   harboring the anthrax toxin genes.";
RL   J. Bacteriol. 181:6509-6515(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ames / isolate Florida / A2012;
RX   PubMed=12004073; DOI=10.1126/science.1071837;
RA   Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L.,
RA   Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P.,
RA   Fraser C.M.;
RT   "Comparative genome sequencing for discovery of novel polymorphisms in
RT   Bacillus anthracis.";
RL   Science 296:2028-2033(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Carbosap, and Ferrara;
RX   PubMed=12067380; DOI=10.1046/j.1365-2672.2002.01660.x;
RA   Adone R., Pasquali P., La Rosa G., Marianelli C., Muscillo M.,
RA   Fasanella A., Francia M., Ciuchini F.;
RT   "Sequence analysis of the genes encoding for the major virulence factors of
RT   Bacillus anthracis vaccine strain 'Carbosap'.";
RL   J. Appl. Microbiol. 93:117-121(2002).
CC   -!- FUNCTION: May allow B.anthracis to germinate within phagocytic cells
CC       and therefore involved in virulence.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Transcription is restricted to the sporulation
CC       phase.
CC   -!- SIMILARITY: Belongs to the GerABKA family. {ECO:0000305}.
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DR   EMBL; AF108144; AAC99415.1; -; Genomic_DNA.
DR   EMBL; AF065404; AAD32417.1; -; Genomic_DNA.
DR   EMBL; AE011190; AAM26102.1; -; Genomic_DNA.
DR   EMBL; AE017336; AAT28898.2; -; Genomic_DNA.
DR   EMBL; AJ413932; CAC93927.1; -; Genomic_DNA.
DR   EMBL; AJ413933; CAC93930.1; -; Genomic_DNA.
DR   PIR; A59105; A59105.
DR   RefSeq; NP_052809.1; NC_001496.1.
DR   RefSeq; WP_000831868.1; NZ_VTZH01000015.1.
DR   AlphaFoldDB; Q9ZFB4; -.
DR   SMR; Q9ZFB4; -.
DR   EnsemblBacteria; AAT28898; AAT28898; GBAA_pXO1_0157.
DR   GeneID; 45025509; -.
DR   KEGG; bar:GBAA_pXO1_0157; -.
DR   HOGENOM; CLU_021639_4_1_9; -.
DR   OMA; FIYCEEL; -.
DR   Proteomes; UP000000594; Plasmid pXO1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009847; P:spore germination; IEA:InterPro.
DR   InterPro; IPR004995; Spore_Ger.
DR   Pfam; PF03323; GerA; 1.
DR   PIRSF; PIRSF005690; GerBA; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Germination; Membrane; Plasmid; Reference proteome;
KW   Transmembrane; Transmembrane helix; Virulence.
FT   CHAIN           1..492
FT                   /note="Spore germination protein XA"
FT                   /id="PRO_0000164018"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        285..305
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        325..345
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        353..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        377..397
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        413..433
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        442..462
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   492 AA;  55142 MW;  007685A07660437C CRC64;
     MKRTVEVNES ILRVWFEGCK DVKIMNRKWC ADTTTTTILL VYCQHVIDHT KLKQAIAPEM
     CNDLLQSSFK DSNLLASNSQ FSVTTLELEN SNENVSRMLF EGKLLIIFQE YKRGYTIDIA
     KLPTRSIEQS NTEMTIRGSR DGFVEELSTN IGLIRKRLKT SSLSYDEFII GERTQTKVGL
     LYLKDVASQE TISQVQFKLK EINIDGVVSS AQIEEFITGD QFSLFPLIEY TGRPDYAVNC
     LLHGRFILLV DGSPTATIAP VSFPFFVNTA EDQNYFYLFG SFVRLLSLFG IAISIFLPGF
     WVALVTYHPD QIPYTLLATL SLSREGIPFP APLEGMIMIT LFELLRQAGL RIPAAFGQTL
     SVVGGLIIGQ AAISSGFVSP SMVVMIAISV VSTFTLVNQS FTGTLSILRY GVFLMSSFLG
     IVGFICSILL IVIHVANLRS FGLPFLAPYS PPVFSSMLPS TFRIPFTRMK KRPKELHTYD
     NTRQRTNNDE NK