GET1_ARATH
ID GET1_ARATH Reviewed; 178 AA.
AC Q1H5D2; Q8LBT2;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Protein GET1 {ECO:0000303|PubMed:28096354};
DE Short=AtGET1 {ECO:0000303|PubMed:28096354};
DE AltName: Full=Guided entry of tail-anchored proteins 1 homolog {ECO:0000305};
GN Name=GET1 {ECO:0000303|PubMed:28096354};
GN OrderedLocusNames=At4g16444 {ECO:0000312|Araport:AT4G16444};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, HOMODIMERIZATION, INTERACTION WITH GET3A, SUBCELLULAR LOCATION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=28096354; DOI=10.1073/pnas.1619525114;
RA Xing S., Mehlhorn D.G., Wallmeroth N., Asseck L.Y., Kar R., Voss A.,
RA Denninger P., Schmidt V.A., Schwarzlaender M., Stierhof Y.D., Grossmann G.,
RA Grefen C.;
RT "Loss of GET pathway orthologs in Arabidopsis thaliana causes root hair
RT growth defects and affects SNARE abundance.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:E1544-E1553(2017).
CC -!- FUNCTION: Required for the post-translational delivery of tail-anchored
CC (TA) proteins to the endoplasmic reticulum. Acts as a membrane receptor
CC for soluble GET3, which recognizes and selectively binds the
CC transmembrane domain of TA proteins in the cytosol (By similarity).
CC Involved in the control of root hair growth through the regulation of
CC syntaxin SYP123 expression (PubMed:28096354).
CC {ECO:0000250|UniProtKB:P53192, ECO:0000269|PubMed:28096354}.
CC -!- SUBUNIT: Forms homodimers (PubMed:28096354). Interacts with GET3A
CC (PubMed:28096354). {ECO:0000269|PubMed:28096354}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:28096354}.
CC -!- DISRUPTION PHENOTYPE: Strong reduction of root hair length.
CC {ECO:0000269|PubMed:28096354}.
CC -!- SIMILARITY: Belongs to the WRB/GET1 family. {ECO:0000305}.
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DR EMBL; AL161544; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002687; AEE83750.1; -; Genomic_DNA.
DR EMBL; BT025532; ABF58950.1; -; mRNA.
DR EMBL; AK227418; BAE99422.1; -; mRNA.
DR EMBL; AY087009; AAM64570.1; -; mRNA.
DR RefSeq; NP_567498.1; NM_117741.4.
DR AlphaFoldDB; Q1H5D2; -.
DR SMR; Q1H5D2; -.
DR IntAct; Q1H5D2; 31.
DR STRING; 3702.AT4G16444.1; -.
DR TCDB; 3.A.19.2.2; the guided entry of tail anchored protein (get) family.
DR PaxDb; Q1H5D2; -.
DR PRIDE; Q1H5D2; -.
DR ProteomicsDB; 178826; -.
DR EnsemblPlants; AT4G16444.1; AT4G16444.1; AT4G16444.
DR GeneID; 827341; -.
DR Gramene; AT4G16444.1; AT4G16444.1; AT4G16444.
DR KEGG; ath:AT4G16444; -.
DR Araport; AT4G16444; -.
DR TAIR; locus:505006480; AT4G16444.
DR eggNOG; ENOG502RZ1V; Eukaryota.
DR HOGENOM; CLU_126784_0_0_1; -.
DR InParanoid; Q1H5D2; -.
DR OMA; RVSKFVC; -.
DR OrthoDB; 1498067at2759; -.
DR PhylomeDB; Q1H5D2; -.
DR PRO; PR:Q1H5D2; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q1H5D2; baseline and differential.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:TAIR.
DR GO; GO:0043529; C:GET complex; IPI:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043621; F:protein self-association; IPI:TAIR.
DR GO; GO:0048767; P:root hair elongation; IMP:TAIR.
DR GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; IEA:InterPro.
DR InterPro; IPR028945; Get1.
DR Pfam; PF04420; CHD5; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Receptor; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..178
FT /note="Protein GET1"
FT /id="PRO_0000449807"
FT TOPO_DOM 1..11
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..110
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..143
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..178
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CONFLICT 20
FT /note="V -> F (in Ref. 5; AAM64570)"
FT /evidence="ECO:0000305"
FT CONFLICT 32
FT /note="L -> F (in Ref. 5; AAM64570)"
FT /evidence="ECO:0000305"
FT CONFLICT 166
FT /note="N -> T (in Ref. 5; AAM64570)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 178 AA; 20206 MW; 40421D38D654E5A8 CRC64;
MEGEKLIEDR GFLAAPLTFV VVVVFQLLSK WLDQLKKKGS KNTRESELRT EIKQLLREAS
ALSQPATFAQ AAKLRRSAAT KEKELAQYLE QHHKEIKLSY DMYGKGLLAS KVVIYLILVL
CFWRTPIAII AKQLVQPFGT LLSWGTGGHM TGHVMVGIIP WLILSNRVSK YVCRFVEF
