ID   GET1_BOVIN              Reviewed;         174 AA.
AC   Q3SZ26;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Guided entry of tail-anchored proteins factor 1 {ECO:0000250|UniProtKB:O00258};
DE   AltName: Full=Tail-anchored protein insertion receptor WRB;
DE   AltName: Full=Tryptophan-rich basic protein;
GN   Name=GET1 {ECO:0000250|UniProtKB:O00258};
GN   Synonyms=WRB {ECO:0000250|UniProtKB:O00258};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Hypothalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for the post-translational delivery of tail-anchored
CC       (TA) proteins to the endoplasmic reticulum. Together with CAMLG/GET2,
CC       acts as a membrane receptor for soluble GET3/TRC40, which recognizes
CC       and selectively binds the transmembrane domain of TA proteins in the
CC       cytosol. Required to ensure correct topology and ER insertion of CAMLG.
CC       {ECO:0000250|UniProtKB:O00258}.
CC   -!- SUBUNIT: Component of the Golgi to ER traffic (GET) complex, which is
CC       composed of GET1/WRB, CAMLG/GET2 and GET3. Within the complex, GET1 and
CC       CAMLG form a heterotetramer which is stabilized by phosphatidylinositol
CC       binding and which binds to the GET3 homodimer. Interacts with CAMLG
CC       (via C-terminus). GET3 shows a higher affinity for CAMLG than for GET1.
CC       {ECO:0000250|UniProtKB:O00258}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:O00258}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the WRB/GET1 family. {ECO:0000305}.
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DR   EMBL; BC103216; AAI03217.1; -; mRNA.
DR   RefSeq; NP_001029531.1; NM_001034359.2.
DR   AlphaFoldDB; Q3SZ26; -.
DR   SMR; Q3SZ26; -.
DR   STRING; 9913.ENSBTAP00000018116; -.
DR   PaxDb; Q3SZ26; -.
DR   PRIDE; Q3SZ26; -.
DR   Ensembl; ENSBTAT00000018116; ENSBTAP00000018116; ENSBTAG00000013629.
DR   GeneID; 509739; -.
DR   KEGG; bta:509739; -.
DR   CTD; 7485; -.
DR   VEuPathDB; HostDB:ENSBTAG00000013629; -.
DR   VGNC; VGNC:36970; GET1.
DR   eggNOG; KOG4253; Eukaryota.
DR   GeneTree; ENSGT00390000008610; -.
DR   HOGENOM; CLU_121992_0_0_1; -.
DR   InParanoid; Q3SZ26; -.
DR   OMA; WFGPLTW; -.
DR   OrthoDB; 1498067at2759; -.
DR   TreeFam; TF314708; -.
DR   Proteomes; UP000009136; Chromosome 1.
DR   Bgee; ENSBTAG00000013629; Expressed in oocyte and 105 other tissues.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043529; C:GET complex; IEA:Ensembl.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0050821; P:protein stabilization; IEA:Ensembl.
DR   GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; IBA:GO_Central.
DR   Gene3D; 1.10.287.660; -; 1.
DR   InterPro; IPR028945; Get1.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   Pfam; PF04420; CHD5; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..174
FT                   /note="Guided entry of tail-anchored proteins factor 1"
FT                   /id="PRO_0000238914"
FT   TOPO_DOM        1..8
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..99
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        121..148
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        170..174
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          39..97
FT                   /note="Interaction with GET3/TRC40"
FT                   /evidence="ECO:0000250"
FT   COILED          39..94
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   174 AA;  19843 MW;  123302A33EBDD6A0 CRC64;
     MSAAEADRWA WLLVLSFVFG CNVLRILLPS FSFFMSRVLQ KDAEQESQMR AEIQGMKQEL
     STVNMMDEFA RYARLERKIN KMTDKLKTHV KARTAQLAKI KWVISVAFYI LQAALMVSLI
     WKYYSVPVAV VPSKWITPLD RLVAFPTRVA GGVGITCWIL VCNKVVAIVL HPFS