ID   GET1_DANRE              Reviewed;         170 AA.
AC   Q6DRM0;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Guided entry of tail-anchored proteins factor 1 {ECO:0000250|UniProtKB:O00258};
DE   AltName: Full=Tail-anchored protein insertion receptor WRB;
DE   AltName: Full=Tryptophan-rich basic protein;
GN   Name=get1 {ECO:0000250|UniProtKB:O00258};
GN   Synonyms=wrb {ECO:0000250|UniProtKB:O00258};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15256591; DOI=10.1073/pnas.0403929101;
RA   Amsterdam A., Nissen R.M., Sun Z., Swindell E.C., Farrington S.,
RA   Hopkins N.;
RT   "Identification of 315 genes essential for early zebrafish development.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12792-12797(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for the post-translational delivery of tail-anchored
CC       (TA) proteins to the endoplasmic reticulum (ER) (By similarity).
CC       Together with CAMLG/GET2, acts as a membrane receptor for soluble
CC       GET3/TRC40, which recognizes and selectively binds the transmembrane
CC       domain of TA proteins in the cytosol (By similarity). Required to
CC       ensure correct topology and ER insertion of CAMLG (By similarity).
CC       {ECO:0000250|UniProtKB:O00258}.
CC   -!- SUBUNIT: Component of the Golgi to ER traffic (GET) complex, which is
CC       composed of GET1/WRB, CAMLG/GET2 and GET3/TRC40. Within the complex,
CC       GET1 and CAMLG form a heterotetramer which is stabilized by
CC       phosphatidylinositol binding and which binds to the GET3 homodimer.
CC       {ECO:0000250|UniProtKB:O00258}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:O00258}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the WRB/GET1 family. {ECO:0000305}.
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DR   EMBL; BC150353; AAI50354.1; -; mRNA.
DR   EMBL; AY648739; AAT68057.1; -; mRNA.
DR   RefSeq; NP_001003413.1; NM_001003413.1.
DR   AlphaFoldDB; Q6DRM0; -.
DR   SMR; Q6DRM0; -.
DR   STRING; 7955.ENSDARP00000100769; -.
DR   PaxDb; Q6DRM0; -.
DR   Ensembl; ENSDART00000114134; ENSDARP00000100769; ENSDARG00000074271.
DR   GeneID; 335756; -.
DR   KEGG; dre:335756; -.
DR   CTD; 7485; -.
DR   ZFIN; ZDB-GENE-030131-7696; get1.
DR   eggNOG; KOG4253; Eukaryota.
DR   GeneTree; ENSGT00940000165905; -.
DR   HOGENOM; CLU_121992_0_0_1; -.
DR   InParanoid; Q6DRM0; -.
DR   OMA; WFGPLTW; -.
DR   OrthoDB; 1498067at2759; -.
DR   PhylomeDB; Q6DRM0; -.
DR   TreeFam; TF314708; -.
DR   PRO; PR:Q6DRM0; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 15.
DR   Bgee; ENSDARG00000074271; Expressed in testis and 28 other tissues.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:MGI.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0007507; P:heart development; IMP:ZFIN.
DR   GO; GO:0010996; P:response to auditory stimulus; IMP:ZFIN.
DR   GO; GO:0060041; P:retina development in camera-type eye; IMP:ZFIN.
DR   GO; GO:0007605; P:sensory perception of sound; IDA:MGI.
DR   GO; GO:0001964; P:startle response; IMP:MGI.
DR   GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; IMP:ZFIN.
DR   Gene3D; 1.10.287.660; -; 1.
DR   InterPro; IPR028945; Get1.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   Pfam; PF04420; CHD5; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..170
FT                   /note="Guided entry of tail-anchored proteins factor 1"
FT                   /id="PRO_0000360887"
FT   TOPO_DOM        1..6
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        28..96
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        118..137
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        159..170
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          35..93
FT                   /note="Interaction with GET3/TRC40"
FT                   /evidence="ECO:0000250"
FT   COILED          61..91
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   170 AA;  19219 MW;  4F4350EB2C29184E CRC64;
     MAAGFNWFLV LSSVFLCNLV KTFLPSISSF LSKIFHKDAD QEMEMRTEIQ NMKMELSTIS
     MMDEFARYAR LERKINKMTD QLKTLVKSRT AQQAKMKWIV NIAFYILQAA LMISLILKYY
     ADPVTVVPSK WIAPLERLVA FPSGVAGGVG ITCWLVVCNK VVALILQAVS