ID   GET1_MAGO7              Reviewed;         215 AA.
AC   A4RHX3; G4MTM0;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-JAN-2012, sequence version 2.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Protein GET1 {ECO:0000255|HAMAP-Rule:MF_03113};
DE   AltName: Full=Guided entry of tail-anchored proteins 1 {ECO:0000255|HAMAP-Rule:MF_03113};
GN   Name=GET1 {ECO:0000255|HAMAP-Rule:MF_03113}; ORFNames=MGG_07181;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
CC   -!- FUNCTION: Required for the post-translational delivery of tail-anchored
CC       (TA) proteins to the endoplasmic reticulum. Acts as a membrane receptor
CC       for soluble GET3, which recognizes and selectively binds the
CC       transmembrane domain of TA proteins in the cytosol. {ECO:0000255|HAMAP-
CC       Rule:MF_03113}.
CC   -!- SUBUNIT: Interacts with GET3. {ECO:0000255|HAMAP-Rule:MF_03113}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000255|HAMAP-Rule:MF_03113}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_03113}.
CC   -!- SIMILARITY: Belongs to the WRB/GET1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03113}.
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DR   EMBL; CM001232; EHA55578.1; -; Genomic_DNA.
DR   RefSeq; XP_003715385.1; XM_003715337.1.
DR   AlphaFoldDB; A4RHX3; -.
DR   SMR; A4RHX3; -.
DR   STRING; 318829.MGG_07181T0; -.
DR   EnsemblFungi; MGG_07181T0; MGG_07181T0; MGG_07181.
DR   GeneID; 2683164; -.
DR   KEGG; mgr:MGG_07181; -.
DR   VEuPathDB; FungiDB:MGG_07181; -.
DR   eggNOG; KOG4253; Eukaryota.
DR   HOGENOM; CLU_089418_1_0_1; -.
DR   InParanoid; A4RHX3; -.
DR   OMA; WFGPLTW; -.
DR   OrthoDB; 1498067at2759; -.
DR   Proteomes; UP000009058; Chromosome 2.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:PAMGO_MGG.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043529; C:GET complex; ISS:PAMGO_MGG.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006874; P:cellular calcium ion homeostasis; ISS:PAMGO_MGG.
DR   GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; ISS:PAMGO_MGG.
DR   GO; GO:0007005; P:mitochondrion organization; ISS:PAMGO_MGG.
DR   GO; GO:0006486; P:protein glycosylation; ISS:PAMGO_MGG.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; ISS:PAMGO_MGG.
DR   GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; IEA:InterPro.
DR   Gene3D; 1.10.287.660; -; 1.
DR   HAMAP; MF_03113; Get1; 1.
DR   InterPro; IPR028945; Get1.
DR   InterPro; IPR027538; Get1_fungi.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   Pfam; PF04420; CHD5; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..215
FT                   /note="Protein GET1"
FT                   /id="PRO_0000388599"
FT   TOPO_DOM        1..4
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TRANSMEM        5..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TOPO_DOM        25..110
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TOPO_DOM        132..155
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TRANSMEM        156..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TOPO_DOM        173..215
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   COILED          72..104
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
SQ   SEQUENCE   215 AA;  24583 MW;  0C7C5F355B2A224E CRC64;
     MPSLLILIFT IEVAVELINT IGAATINNLL WRIFNALPTK LSAQFAEQRK LQQDYLKVRR
     ELNATSSQDE FAKWAKLRRQ HDKLLEQLEK KKAALDSTKG NFDKYITGIR WVGTQGLRYF
     LPFWYAKVPM FWLPYGWFPY YAEWLVSFPR APMGSVSIAS WQLACTGFVV LIKDAITALV
     VFVMGMRQSN VKQAVPVKAV SGEKASDEKE GKKEL