GET1_MOUSE
ID GET1_MOUSE Reviewed; 174 AA.
AC Q8K0D7; Q3TA55; Q9D1W0;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Guided entry of tail-anchored proteins factor 1 {ECO:0000312|MGI:MGI:2136882};
DE AltName: Full=Tail-anchored protein insertion receptor WRB;
DE AltName: Full=Tryptophan-rich basic protein;
GN Name=Get1 {ECO:0000312|MGI:MGI:2136882};
GN Synonyms=Wrb {ECO:0000312|MGI:MGI:2136882};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Required for the post-translational delivery of tail-anchored
CC (TA) proteins to the endoplasmic reticulum. Together with CAMLG/GET2,
CC acts as a membrane receptor for soluble GET3/TRC40, which recognizes
CC and selectively binds the transmembrane domain of TA proteins in the
CC cytosol. Required to ensure correct topology and ER insertion of CAMLG.
CC {ECO:0000250|UniProtKB:O00258}.
CC -!- SUBUNIT: Component of the Golgi to ER traffic (GET) complex, which is
CC composed of GET1, CAMLG/GET2 and GET3. Within the complex, GET1 and
CC CAMLG form a heterotetramer which is stabilized by phosphatidylinositol
CC binding and which binds to the GET3 homodimer. Interacts with
CC CAMLG/GET2 (via C-terminus). GET3 shows a higher affinity for CAMLG
CC than for GET1. {ECO:0000250|UniProtKB:O00258}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:O00258}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the WRB/GET1 family. {ECO:0000305}.
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DR EMBL; AK172079; BAE42815.1; -; mRNA.
DR EMBL; BC031769; AAH31769.1; -; mRNA.
DR CCDS; CCDS28354.1; -.
DR RefSeq; NP_997184.1; NM_207301.2.
DR AlphaFoldDB; Q8K0D7; -.
DR SMR; Q8K0D7; -.
DR BioGRID; 214712; 4.
DR STRING; 10090.ENSMUSP00000023913; -.
DR PhosphoSitePlus; Q8K0D7; -.
DR EPD; Q8K0D7; -.
DR MaxQB; Q8K0D7; -.
DR PaxDb; Q8K0D7; -.
DR PeptideAtlas; Q8K0D7; -.
DR PRIDE; Q8K0D7; -.
DR ProteomicsDB; 275218; -.
DR DNASU; 71446; -.
DR Ensembl; ENSMUST00000023913; ENSMUSP00000023913; ENSMUSG00000023147.
DR GeneID; 71446; -.
DR KEGG; mmu:71446; -.
DR UCSC; uc008acp.1; mouse.
DR CTD; 7485; -.
DR MGI; MGI:2136882; Get1.
DR VEuPathDB; HostDB:ENSMUSG00000023147; -.
DR eggNOG; KOG4253; Eukaryota.
DR GeneTree; ENSGT01050000248375; -.
DR HOGENOM; CLU_121992_0_0_1; -.
DR InParanoid; Q8K0D7; -.
DR OMA; WFGPLTW; -.
DR OrthoDB; 1498067at2759; -.
DR PhylomeDB; Q8K0D7; -.
DR TreeFam; TF314708; -.
DR BioGRID-ORCS; 71446; 27 hits in 76 CRISPR screens.
DR ChiTaRS; Wrb; mouse.
DR PRO; PR:Q8K0D7; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8K0D7; protein.
DR Bgee; ENSMUSG00000023147; Expressed in floor plate of midbrain and 247 other tissues.
DR ExpressionAtlas; Q8K0D7; baseline and differential.
DR Genevisible; Q8K0D7; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043529; C:GET complex; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071599; P:otic vesicle development; IMP:MGI.
DR GO; GO:0006620; P:post-translational protein targeting to endoplasmic reticulum membrane; IMP:MGI.
DR GO; GO:0045048; P:protein insertion into ER membrane; ISS:UniProtKB.
DR GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
DR GO; GO:0007605; P:sensory perception of sound; IMP:MGI.
DR GO; GO:0001964; P:startle response; ISO:MGI.
DR GO; GO:0050808; P:synapse organization; IMP:MGI.
DR GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
DR Gene3D; 1.10.287.660; -; 1.
DR InterPro; IPR028945; Get1.
DR InterPro; IPR029012; Helix_hairpin_bin_sf.
DR Pfam; PF04420; CHD5; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endoplasmic reticulum; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..174
FT /note="Guided entry of tail-anchored proteins factor 1"
FT /id="PRO_0000065980"
FT TOPO_DOM 1..8
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..148
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 39..97
FT /note="Interaction with GET3/TRC40"
FT /evidence="ECO:0000250"
FT COILED 39..94
FT /evidence="ECO:0000255"
SQ SEQUENCE 174 AA; 19867 MW; E0ACE1A6951E4865 CRC64;
MSASETDRWA WLLVLSFVFG CNLLRILLPS LSSFISRVLQ KDAEQESQMR AEIQGMKQEL
STVNMMDEFA RYARLERKIN KMTDKLKTHV KARTAQLAKI KWFISVAFYI LQAALMISLI
WKYYSVPVAV VPSKWITPLD RLVAFPTRVA GGIGITCWIL VCNKVVAIVL HPFS
