ID   GET1_PONAB              Reviewed;         174 AA.
AC   Q5R6K7;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Guided entry of tail-anchored proteins factor 1 {ECO:0000250|UniProtKB:O00258};
DE   AltName: Full=Tail-anchored protein insertion receptor WRB;
DE   AltName: Full=Tryptophan-rich basic protein;
GN   Name=GET1 {ECO:0000250|UniProtKB:O00258};
GN   Synonyms=WRB {ECO:0000250|UniProtKB:O00258};
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for the post-translational delivery of tail-anchored
CC       (TA) proteins to the endoplasmic reticulum. Together with CAMLG/GET2,
CC       acts as a membrane receptor for soluble GET3/TRC40, which recognizes
CC       and selectively binds the transmembrane domain of TA proteins in the
CC       cytosol. Required to ensure correct topology and ER insertion of CAMLG.
CC       {ECO:0000250|UniProtKB:O00258}.
CC   -!- SUBUNIT: Component of the Golgi to ER traffic (GET) complex, which is
CC       composed of GET1/WRB, CAMLG/GET2 and GET3. Within the complex, GET1 and
CC       CAMLG form a heterotetramer which is stabilized by phosphatidylinositol
CC       binding and which binds to the GET3 homodimer. Interacts with CAMLG
CC       (via C-terminus). GET3 shows a higher affinity for CAMLG than for GET1.
CC       {ECO:0000250|UniProtKB:O00258}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:O00258}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the WRB/GET1 family. {ECO:0000305}.
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DR   EMBL; CR860481; CAH92603.1; -; mRNA.
DR   RefSeq; NP_001126525.1; NM_001133053.1.
DR   AlphaFoldDB; Q5R6K7; -.
DR   SMR; Q5R6K7; -.
DR   STRING; 9601.ENSPPYP00000012754; -.
DR   GeneID; 100173514; -.
DR   KEGG; pon:100173514; -.
DR   CTD; 7485; -.
DR   eggNOG; KOG4253; Eukaryota.
DR   InParanoid; Q5R6K7; -.
DR   OrthoDB; 1498067at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043529; C:GET complex; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0045048; P:protein insertion into ER membrane; ISS:UniProtKB.
DR   GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
DR   GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
DR   Gene3D; 1.10.287.660; -; 1.
DR   InterPro; IPR028945; Get1.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   Pfam; PF04420; CHD5; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..174
FT                   /note="Guided entry of tail-anchored proteins factor 1"
FT                   /id="PRO_0000065981"
FT   TOPO_DOM        1..8
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..99
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        121..148
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        170..174
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          39..97
FT                   /note="Interaction with GET3/TRC40"
FT                   /evidence="ECO:0000250"
FT   COILED          39..94
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   174 AA;  19766 MW;  0F1F0F3DB1D38202 CRC64;
     MSSAAADHWA WLLVLSFVFG CNVLRVLLPS FSSFMSRVLQ KDAEQESQMR AEIQDMKQEL
     STVNMMDEFA RYARLERKIN KMTDKLKTHV KARTAQLAKI KWVISVAFYV LQAALMISLI
     WKYYSVPVAV VPSKWITPLD RLVAFPTRVA GGVGITCWIL VCNKVVAIVL HPFS