GET1_RAT
ID GET1_RAT Reviewed; 174 AA.
AC Q6P6S5;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Guided entry of tail-anchored proteins factor 1 {ECO:0000312|RGD:735104};
DE AltName: Full=Tail-anchored protein insertion receptor WRB;
DE AltName: Full=Tryptophan-rich basic protein;
GN Name=Get1 {ECO:0000312|RGD:735104}; Synonyms=Wrb {ECO:0000312|RGD:735104};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP FUNCTION, IDENTIFICATION IN GET COMPLEX, AND INTERACTION WITH CAMLG AND
RP GET3.
RX PubMed=23041287; DOI=10.1016/j.molcel.2012.08.028;
RA Yamamoto Y., Sakisaka T.;
RT "Molecular machinery for insertion of tail-anchored membrane proteins into
RT the endoplasmic reticulum membrane in mammalian cells.";
RL Mol. Cell 48:387-397(2012).
CC -!- FUNCTION: Required for the post-translational delivery of tail-anchored
CC (TA) proteins to the endoplasmic reticulum (PubMed:23041287). Together
CC with CAMLG/GET2, acts as a membrane receptor for soluble GET3/TRC40,
CC which recognizes and selectively binds the transmembrane domain of TA
CC proteins in the cytosol (PubMed:23041287). Required to ensure correct
CC topology and ER insertion of CAMLG (By similarity).
CC {ECO:0000250|UniProtKB:O00258, ECO:0000269|PubMed:23041287}.
CC -!- SUBUNIT: Component of the Golgi to ER traffic (GET) complex, which is
CC composed of GET1, CAMLG/GET2 and GET3 (PubMed:23041287). Within the
CC complex, GET1 and CAMLG form a heterotetramer which is stabilized by
CC phosphatidylinositol binding and which binds to the GET3 homodimer (By
CC similarity). Interacts with CAMLG/GET2 (via C-terminus)
CC (PubMed:23041287). GET3 shows a higher affinity for CAMLG than for GET1
CC (By similarity). {ECO:0000250|UniProtKB:O00258,
CC ECO:0000269|PubMed:23041287}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:O00258}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the WRB/GET1 family. {ECO:0000305}.
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DR EMBL; BC062049; AAH62049.1; -; mRNA.
DR RefSeq; NP_955405.1; NM_199373.1.
DR AlphaFoldDB; Q6P6S5; -.
DR SMR; Q6P6S5; -.
DR STRING; 10116.ENSRNOP00000002222; -.
DR jPOST; Q6P6S5; -.
DR PaxDb; Q6P6S5; -.
DR PRIDE; Q6P6S5; -.
DR Ensembl; ENSRNOT00000002222; ENSRNOP00000002222; ENSRNOG00000001629.
DR GeneID; 288233; -.
DR KEGG; rno:288233; -.
DR UCSC; RGD:735104; rat.
DR CTD; 7485; -.
DR RGD; 735104; Get1.
DR eggNOG; KOG4253; Eukaryota.
DR GeneTree; ENSGT01050000248375; -.
DR HOGENOM; CLU_121992_0_0_1; -.
DR InParanoid; Q6P6S5; -.
DR OMA; WFGPLTW; -.
DR OrthoDB; 1498067at2759; -.
DR PhylomeDB; Q6P6S5; -.
DR TreeFam; TF314708; -.
DR PRO; PR:Q6P6S5; -.
DR Proteomes; UP000002494; Chromosome 11.
DR Bgee; ENSRNOG00000001629; Expressed in heart and 19 other tissues.
DR Genevisible; Q6P6S5; RN.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043529; C:GET complex; IPI:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071599; P:otic vesicle development; ISO:RGD.
DR GO; GO:0006620; P:post-translational protein targeting to endoplasmic reticulum membrane; ISO:RGD.
DR GO; GO:0045048; P:protein insertion into ER membrane; ISO:RGD.
DR GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
DR GO; GO:0007605; P:sensory perception of sound; ISO:RGD.
DR GO; GO:0050808; P:synapse organization; ISO:RGD.
DR GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; IDA:UniProtKB.
DR Gene3D; 1.10.287.660; -; 1.
DR InterPro; IPR028945; Get1.
DR InterPro; IPR029012; Helix_hairpin_bin_sf.
DR Pfam; PF04420; CHD5; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endoplasmic reticulum; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..174
FT /note="Guided entry of tail-anchored proteins factor 1"
FT /id="PRO_0000065982"
FT TOPO_DOM 1..8
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..148
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 39..97
FT /note="Interaction with GET3/TRC40"
FT /evidence="ECO:0000250"
FT COILED 39..94
FT /evidence="ECO:0000255"
SQ SEQUENCE 174 AA; 19899 MW; 7941B91831F2DC6B CRC64;
MSASETDRWA WLLVLCFVFG CNVLRILLPT LSSFISRVLQ KDAEQESQMR AEIQSMKQEL
STVNMMDEFA RYARLERKIN KMTDKLKTHV KARTAQLAKI KWFISVAFYV LQAALMISLI
WKYYSVPVAV VPSKWITPLD RLVAFPTRVA GGIGVTCWIL VCNKVVAIIL HPFS
