ID   GET1_TALSN              Reviewed;         200 AA.
AC   B8LUE3;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Protein get1 {ECO:0000255|HAMAP-Rule:MF_03113};
DE   AltName: Full=Guided entry of tail-anchored proteins 1 {ECO:0000255|HAMAP-Rule:MF_03113};
GN   Name=get1; ORFNames=TSTA_071190;
OS   Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL
OS   1006) (Penicillium stipitatum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces;
OC   Talaromyces sect. Talaromyces.
OX   NCBI_TaxID=441959;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006;
RX   PubMed=25676766; DOI=10.1128/genomea.01559-14;
RA   Nierman W.C., Fedorova-Abrams N.D., Andrianopoulos A.;
RT   "Genome sequence of the AIDS-associated pathogen Penicillium marneffei
RT   (ATCC18224) and its near taxonomic relative Talaromyces stipitatus
RT   (ATCC10500).";
RL   Genome Announc. 3:E0155914-E0155914(2015).
CC   -!- FUNCTION: Required for the post-translational delivery of tail-anchored
CC       (TA) proteins to the endoplasmic reticulum. Acts as a membrane receptor
CC       for soluble get3, which recognizes and selectively binds the
CC       transmembrane domain of TA proteins in the cytosol. {ECO:0000255|HAMAP-
CC       Rule:MF_03113}.
CC   -!- SUBUNIT: Interacts with get3. {ECO:0000255|HAMAP-Rule:MF_03113}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000255|HAMAP-Rule:MF_03113}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_03113}.
CC   -!- SIMILARITY: Belongs to the WRB/GET1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03113}.
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DR   EMBL; EQ962652; EED23716.1; -; Genomic_DNA.
DR   RefSeq; XP_002341103.1; XM_002341062.1.
DR   AlphaFoldDB; B8LUE3; -.
DR   SMR; B8LUE3; -.
DR   STRING; 441959.B8LUE3; -.
DR   EnsemblFungi; EED23716; EED23716; TSTA_071190.
DR   GeneID; 8106016; -.
DR   VEuPathDB; FungiDB:TSTA_071190; -.
DR   eggNOG; KOG4253; Eukaryota.
DR   HOGENOM; CLU_089418_1_0_1; -.
DR   InParanoid; B8LUE3; -.
DR   OMA; WFGPLTW; -.
DR   OrthoDB; 1498067at2759; -.
DR   PhylomeDB; B8LUE3; -.
DR   Proteomes; UP000001745; Unassembled WGS sequence.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043529; C:GET complex; IEA:InterPro.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; IEA:InterPro.
DR   Gene3D; 1.10.287.660; -; 1.
DR   HAMAP; MF_03113; Get1; 1.
DR   InterPro; IPR028945; Get1.
DR   InterPro; IPR027538; Get1_fungi.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   Pfam; PF04420; CHD5; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..200
FT                   /note="Protein get1"
FT                   /id="PRO_0000388619"
FT   TOPO_DOM        1..4
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TRANSMEM        5..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TOPO_DOM        25..110
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TOPO_DOM        132..155
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TRANSMEM        156..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   TOPO_DOM        173..200
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
FT   COILED          45..97
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03113"
SQ   SEQUENCE   200 AA;  22690 MW;  1CB771F4867E1319 CRC64;
     MISFLLLIFL IQLAIYIVNT IGASTVDDLL WILYLRLPSS ISKDARKHGE LKRDVVQLKR
     EMNATSSQDE FAKWAKLRRR HDKAMEEYEA MNRSMGSRKT SFQYSVKIAR WLTLNGPRLF
     IQFYYTKTPV FDLPPGWFPY PVEWILSFPR APLGTVSIQV WSSACATAIS LTGNVVIAAL
     QKSGQASMRQ AQAIPAGKSE