ID   GET2_KOMPG              Reviewed;         313 AA.
AC   C4R3U0;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Golgi to ER traffic protein 2 {ECO:0000255|HAMAP-Rule:MF_03114};
GN   Name=GET2 {ECO:0000255|HAMAP-Rule:MF_03114};
GN   OrderedLocusNames=PAS_chr3_0197;
OS   Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=644223;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GS115 / ATCC 20864;
RX   PubMed=19465926; DOI=10.1038/nbt.1544;
RA   De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S.,
RA   Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT   "Genome sequence of the recombinant protein production host Pichia
RT   pastoris.";
RL   Nat. Biotechnol. 27:561-566(2009).
CC   -!- FUNCTION: Required for the post-translational delivery of tail-anchored
CC       (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as
CC       a membrane receptor for soluble GET3, which recognizes and selectively
CC       binds the transmembrane domain of TA proteins in the cytosol. The GET
CC       complex cooperates with the HDEL receptor ERD2 to mediate the ATP-
CC       dependent retrieval of resident ER proteins that contain a C-terminal
CC       H-D-E-L retention signal from the Golgi to the ER. {ECO:0000255|HAMAP-
CC       Rule:MF_03114}.
CC   -!- SUBUNIT: Component of the Golgi to ER traffic (GET) complex, which is
CC       composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form
CC       a heterotetramer which is stabilized by phosphatidylinositol binding
CC       and which binds to the GET3 homodimer. {ECO:0000255|HAMAP-
CC       Rule:MF_03114}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000255|HAMAP-Rule:MF_03114}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_03114}. Golgi apparatus membrane
CC       {ECO:0000255|HAMAP-Rule:MF_03114}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_03114}.
CC   -!- SIMILARITY: Belongs to the GET2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03114}.
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DR   EMBL; FN392321; CAY70182.1; -; Genomic_DNA.
DR   RefSeq; XP_002492405.1; XM_002492360.1.
DR   AlphaFoldDB; C4R3U0; -.
DR   SMR; C4R3U0; -.
DR   STRING; 644223.C4R3U0; -.
DR   EnsemblFungi; CAY70182; CAY70182; PAS_chr3_0197.
DR   GeneID; 8199947; -.
DR   KEGG; ppa:PAS_chr3_0197; -.
DR   eggNOG; ENOG502QW0H; Eukaryota.
DR   HOGENOM; CLU_066477_0_0_1; -.
DR   InParanoid; C4R3U0; -.
DR   OMA; ALQYWDV; -.
DR   Proteomes; UP000000314; Chromosome 3.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043529; C:GET complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0045048; P:protein insertion into ER membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR   HAMAP; MF_03114; Get2; 1.
DR   InterPro; IPR014802; GET2.
DR   InterPro; IPR028143; Get2/sif1.
DR   PANTHER; PTHR28263; PTHR28263; 1.
DR   Pfam; PF08690; GET2; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..313
FT                   /note="Golgi to ER traffic protein 2"
FT                   /id="PRO_0000388636"
FT   TOPO_DOM        1..191
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03114"
FT   TRANSMEM        192..212
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03114"
FT   TOPO_DOM        213..230
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03114"
FT   TRANSMEM        231..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03114"
FT   TOPO_DOM        251..289
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03114"
FT   TRANSMEM        290..310
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03114"
FT   TOPO_DOM        311..313
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03114"
FT   REGION          15..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        36..66
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   313 AA;  34393 MW;  AE5E4F9EAF18356B CRC64;
     MSELTEAEKR QLLREKRKAK LSQGGGLDRL KKITGENNSK LSTDVPTKEP TADATTTATE
     LPTQGLETRI NAHDDPVSEI PDPLNEGFDG KEPDLDQLIA SMFNKSAGHE NPNSEEQGVP
     ELMRRFSSIL QGGDSGAAGL GAEAGGINPL DLLNSLGGSN IGKAQEEYGS TPEEIEFNKK
     SIAYKKHQNE VLKAKILVVR LVLILSLLFV YGRDFSLSLF TQTYAPNGSS FMRVFLTLEL
     IFQTSLFFFI SKNKNFTDDS LISKLLNIGG AFIPATYRNL LQTASKYQVL LSMFLFDLSI
     VVVVFAIRAA FNF