ID   3S11A_OPHHA             Reviewed;          83 AA.
AC   P01412; A8N287;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Weak toxin DE-1;
DE   Flags: Precursor;
OS   Ophiophagus hannah (King cobra) (Naja hannah).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Ophiophagus.
OX   NCBI_TaxID=8665;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=17616557; DOI=10.1096/fj.07-8658com;
RA   Rajagopalan N., Pung Y.F., Zhu Y.Z., Wong P.T.H., Kumar P.P., Kini R.M.;
RT   "Beta-cardiotoxin: a new three-finger toxin from Ophiophagus hannah (king
RT   cobra) venom with beta-blocker activity.";
RL   FASEB J. 21:3685-3695(2007).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-83, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=881163; DOI=10.1515/bchm2.1977.358.1.565;
RA   Joubert F.J.;
RT   "Snake venoms. The amino-acid sequence of polypeptide DE-1 from Ophiophagus
RT   hannah (King cobra) venom.";
RL   Hoppe-Seyler's Z. Physiol. Chem. 358:565-574(1977).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:881163}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(50) is >250 mg/kg by subcutaneous injection.
CC       {ECO:0000269|PubMed:881163}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type I alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR   EMBL; DQ902576; ABK41956.1; -; mRNA.
DR   PIR; A01682; V7OH1D.
DR   AlphaFoldDB; P01412; -.
DR   SMR; P01412; -.
DR   TopDownProteomics; P01412; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:881163"
FT   CHAIN           22..83
FT                   /note="Weak toxin DE-1"
FT                   /evidence="ECO:0000269|PubMed:881163"
FT                   /id="PRO_0000093629"
FT   DISULFID        24..45
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        38..62
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        64..75
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        76..81
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   CONFLICT        43..44
FT                   /note="DT -> N (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        51..55
FT                   /note="NDHHG -> HNDGH (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        73
FT                   /note="Missing (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   83 AA;  9304 MW;  7EE5DA156C866C7D CRC64;
     MKTLLLTLVV VTIVCLDLGY SLICFNQETY RPETTTTCPD GEDTCYSTFW NDHHGVKIER
     GCGCPRVNPP ISIICCKTDK CNN