GET4_ARATH
ID GET4_ARATH Reviewed; 324 AA.
AC Q6GKV1; Q9FMK1;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Protein GET4 {ECO:0000303|PubMed:28096354};
DE Short=AtGET4 {ECO:0000303|PubMed:28096354};
DE AltName: Full=Guided entry of tail-anchored proteins 4 homolog {ECO:0000305};
GN Name=MDC12.19 {ECO:0000312|EMBL:AED97719.1};
GN Synonyms=MDC12_19 {ECO:0000312|EMBL:AED97719.1};
GN OrderedLocusNames=At5g63220 {ECO:0000312|Araport:AT5G63220};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [7]
RP FUNCTION, INTERACTION WITH GET3A, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=28096354; DOI=10.1073/pnas.1619525114;
RA Xing S., Mehlhorn D.G., Wallmeroth N., Asseck L.Y., Kar R., Voss A.,
RA Denninger P., Schmidt V.A., Schwarzlaender M., Stierhof Y.D., Grossmann G.,
RA Grefen C.;
RT "Loss of GET pathway orthologs in Arabidopsis thaliana causes root hair
RT growth defects and affects SNARE abundance.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:E1544-E1553(2017).
CC -!- FUNCTION: Involved in the regulation of root hair growth.
CC {ECO:0000269|PubMed:28096354}.
CC -!- SUBUNIT: Interacts with GET3A. {ECO:0000269|PubMed:28096354}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:28096354}.
CC -!- DISRUPTION PHENOTYPE: Strong reduction of root hair length.
CC {ECO:0000269|PubMed:28096354}.
CC -!- SIMILARITY: Belongs to the GET4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10564.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB008265; BAB10564.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97719.1; -; Genomic_DNA.
DR EMBL; BT014963; AAT47814.1; -; mRNA.
DR EMBL; AK176227; BAD43990.1; -; mRNA.
DR RefSeq; NP_201127.2; NM_125717.6.
DR AlphaFoldDB; Q6GKV1; -.
DR SMR; Q6GKV1; -.
DR STRING; 3702.AT5G63220.1; -.
DR PaxDb; Q6GKV1; -.
DR PRIDE; Q6GKV1; -.
DR ProteomicsDB; 193588; -.
DR DNASU; 836442; -.
DR EnsemblPlants; AT5G63220.1; AT5G63220.1; AT5G63220.
DR GeneID; 836442; -.
DR Gramene; AT5G63220.1; AT5G63220.1; AT5G63220.
DR KEGG; ath:AT5G63220; -.
DR Araport; AT5G63220; -.
DR TAIR; locus:2161917; AT5G63220.
DR eggNOG; KOG3024; Eukaryota.
DR HOGENOM; CLU_046061_1_0_1; -.
DR InParanoid; Q6GKV1; -.
DR OMA; QIGEMYF; -.
DR OrthoDB; 1030907at2759; -.
DR PhylomeDB; Q6GKV1; -.
DR PRO; PR:Q6GKV1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6GKV1; baseline and differential.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0045048; P:protein insertion into ER membrane; IBA:GO_Central.
DR GO; GO:0048767; P:root hair elongation; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR007317; GET4.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR12875; PTHR12875; 1.
DR Pfam; PF04190; GET4; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome.
FT CHAIN 1..324
FT /note="Protein GET4"
FT /id="PRO_0000449811"
SQ SEQUENCE 324 AA; 37177 MW; 7A74D8D71179BC7A CRC64;
MSRERIKREL PPVQEHIDKL RKVIEEGNYY GALQMYKSIS ARYVTAQRFS EALDILFSGA
CIELEHGLVN CGADLAILFV DTLVKAKSPC NDETLDRIRC IFKLFPRVPV PPHLVDVSDD
EDVQNLQESL GEARSRVENL TSFLRAAIKW SAEFGGPRTG YPELHAMLGD YLYTECPELD
MVRISRHFVR AEDPEKFASM LVNFMGRCYP GEDDLAIARA VLMYLSMGNM KDANFMMDEI
KKQAETKNPE LSESDLIQFI SYLLETLQRD ALPLFNMLRV KYKSSIDRDQ LLNELLDEIA
ERFYGVQRKN PLQGMFGDIF KMMG
