GET4_BOVIN
ID GET4_BOVIN Reviewed; 325 AA.
AC Q0P5I8;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Golgi to ER traffic protein 4 homolog;
GN Name=GET4;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal skin;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: As part of a cytosolic protein quality control complex, the
CC BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-
CC containing proteins in a soluble state and participates in their proper
CC delivery to the endoplasmic reticulum or alternatively can promote
CC their sorting to the proteasome where they undergo degradation. The
CC BAG6/BAT3 complex is involved in the post-translational delivery of
CC tail-anchored/type II transmembrane proteins to the endoplasmic
CC reticulum membrane. Recruited to ribosomes, it interacts with the
CC transmembrane region of newly synthesized tail-anchored proteins and
CC together with SGTA and ASNA1 mediates their delivery to the endoplasmic
CC reticulum. Client proteins that cannot be properly delivered to the
CC endoplasmic reticulum are ubiquitinated and sorted to the proteasome.
CC Similarly, the BAG6/BAT3 complex also functions as a sorting platform
CC for proteins of the secretory pathway that are mislocalized to the
CC cytosol either delivering them to the proteasome for degradation or to
CC the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in
CC the endoplasmic reticulum-associated degradation (ERAD), a quality
CC control mechanism that eliminates unwanted proteins of the endoplasmic
CC reticulum through their retrotranslocation to the cytosol and their
CC targeting to the proteasome. It maintains these retrotranslocated
CC proteins in an unfolded yet soluble state condition in the cytosol to
CC ensure their proper delivery to the proteasome.
CC {ECO:0000250|UniProtKB:Q7L5D6}.
CC -!- SUBUNIT: Component of the BAG6/BAT3 complex, at least composed of BAG6,
CC UBL4A and GET4/TRC35. Interacts with BAG6; the interaction is direct
CC and localizes BAG6 in the cytosol. {ECO:0000250|UniProtKB:Q7L5D6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q7L5D6}.
CC -!- PTM: Ubiquitinated by RNF12, leading to proteasomal degradation. When
CC unassembled from BAG6; ubiquitinylation is modulated by BAG6 quality
CC control role and effectuated by RNF126. {ECO:0000250|UniProtKB:Q7L5D6}.
CC -!- SIMILARITY: Belongs to the GET4 family. {ECO:0000305}.
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DR EMBL; BC119983; AAI19984.1; -; mRNA.
DR RefSeq; NP_001069993.1; NM_001076525.1.
DR RefSeq; XP_010817981.1; XM_010819679.1.
DR RefSeq; XP_015324964.1; XM_015469478.1.
DR AlphaFoldDB; Q0P5I8; -.
DR SMR; Q0P5I8; -.
DR STRING; 9913.ENSBTAP00000036940; -.
DR PaxDb; Q0P5I8; -.
DR Ensembl; ENSBTAT00000037101; ENSBTAP00000036940; ENSBTAG00000026191.
DR GeneID; 618954; -.
DR KEGG; bta:618954; -.
DR CTD; 51608; -.
DR VEuPathDB; HostDB:ENSBTAG00000026191; -.
DR VGNC; VGNC:97274; GET4.
DR eggNOG; KOG3024; Eukaryota.
DR GeneTree; ENSGT00390000015750; -.
DR HOGENOM; CLU_046061_2_1_1; -.
DR InParanoid; Q0P5I8; -.
DR OMA; QIGEMYF; -.
DR OrthoDB; 1030907at2759; -.
DR Proteomes; UP000009136; Chromosome 25.
DR Bgee; ENSBTAG00000026191; Expressed in retina and 105 other tissues.
DR GO; GO:0071818; C:BAT3 complex; ISS:UniProtKB.
DR GO; GO:0005694; C:chromosome; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0051087; F:chaperone binding; IEA:Ensembl.
DR GO; GO:0051220; P:cytoplasmic sequestering of protein; IEA:Ensembl.
DR GO; GO:1904378; P:maintenance of unfolded protein involved in ERAD pathway; IEA:Ensembl.
DR GO; GO:0006620; P:post-translational protein targeting to endoplasmic reticulum membrane; IEA:Ensembl.
DR GO; GO:0045048; P:protein insertion into ER membrane; IBA:GO_Central.
DR GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:Ensembl.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR007317; GET4.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR12875; PTHR12875; 1.
DR Pfam; PF04190; GET4; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Phosphoprotein; Reference proteome; Transport; Ubl conjugation.
FT CHAIN 1..325
FT /note="Golgi to ER traffic protein 4 homolog"
FT /id="PRO_0000328386"
FT REGION 194..270
FT /note="Interacts with BAG6"
FT /evidence="ECO:0000250|UniProtKB:Q7L5D6"
FT REGION 306..325
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 311..325
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q7L5D6"
SQ SEQUENCE 325 AA; 36185 MW; 421D002EC67C3A4C CRC64;
MAAAAMAEQE SARNGARNRG GVQRVEGKLR ASVEKGDYYE AHQMYRTLFF RYMAQSKHAE
ARELMCSGAL LFFSHGQQNS AADLSMLVLE SLEKAEVEVA DELLESLAKL FSLMDPNSPE
RVAFVSRALK WSSGGSGKLG HPRLHQLLAL TLWKEQNYCE SRYHFLHSSD GEGCANMLVE
YSTARGFRSE VDMFVAQAVL QFLCLKNKSS ASVVFTTYTQ KHPSIEGGPP FVQPLLNFIW
FLLLAVDGGK LTVFTVLCEQ YQPSLRRDPM YNEYLDRIGQ LFFGVPPKQT SSYGGLLGNL
LSSLMGSSEQ EGEDSQDDSS PIELD
