GET4_YEAST
ID GET4_YEAST Reviewed; 312 AA.
AC Q12125; D6W2M2;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Golgi to ER traffic protein 4;
DE AltName: Full=Guided entry of tail-anchored proteins 4;
GN Name=GET4; OrderedLocusNames=YOR164C; ORFNames=O3580;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / FY1678;
RX PubMed=8972579;
RX DOI=10.1002/(sici)1097-0061(199612)12:15<1563::aid-yea44>3.0.co;2-m;
RA Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.;
RT "Analysis of a 22,956 bp region on the right arm of Saccharomyces
RT cerevisiae chromosome XV.";
RL Yeast 12:1563-1573(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION.
RX PubMed=19325107; DOI=10.1126/science.1167983;
RA Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V.,
RA Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M.;
RT "Comprehensive characterization of genes required for protein folding in
RT the endoplasmic reticulum.";
RL Science 323:1693-1697(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) IN COMPLEX WITH MDY2.
RX PubMed=20106980; DOI=10.1074/jbc.m109.087098;
RA Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D.,
RA Wang C.;
RT "Crystal structure of Get4-Get5 complex and its interactions with Sgt2,
RT Get3, and Ydj1.";
RL J. Biol. Chem. 285:9962-9970(2010).
CC -!- FUNCTION: May play a role in insertion of tail-anchored proteins into
CC the endoplasmic reticulum membrane. {ECO:0000269|PubMed:19325107}.
CC -!- SUBUNIT: Interacts with MDY2/GET5. {ECO:0000269|PubMed:20106980}.
CC -!- INTERACTION:
CC Q12125; Q12154: GET3; NbExp=11; IntAct=EBI-36940, EBI-2989;
CC Q12125; Q12285: MDY2; NbExp=17; IntAct=EBI-36940, EBI-34904;
CC Q12125; P22214: SEC22; NbExp=2; IntAct=EBI-36940, EBI-16577;
CC Q12125; Q12118: SGT2; NbExp=5; IntAct=EBI-36940, EBI-31784;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 5350 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the GET4 family. {ECO:0000305}.
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DR EMBL; U55021; AAB47411.1; -; Genomic_DNA.
DR EMBL; Z75072; CAA99370.1; -; Genomic_DNA.
DR EMBL; AY558036; AAS56362.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10938.1; -; Genomic_DNA.
DR PIR; S67052; S67052.
DR RefSeq; NP_014807.3; NM_001183583.3.
DR PDB; 2WPV; X-ray; 1.99 A; A/C/E/G=1-312.
DR PDB; 3LKU; X-ray; 2.80 A; A/C/E=11-300.
DR PDB; 4PWX; X-ray; 5.40 A; C/E=11-290.
DR PDB; 5BW8; X-ray; 2.80 A; C=1-290.
DR PDB; 5BWK; X-ray; 6.00 A; E/G/I/K/Q/S/U/W=11-311.
DR PDBsum; 2WPV; -.
DR PDBsum; 3LKU; -.
DR PDBsum; 4PWX; -.
DR PDBsum; 5BW8; -.
DR PDBsum; 5BWK; -.
DR AlphaFoldDB; Q12125; -.
DR SMR; Q12125; -.
DR BioGRID; 34560; 267.
DR ComplexPortal; CPX-1861; GET4-GET5 transmembrane domain recognition complex.
DR DIP; DIP-2005N; -.
DR IntAct; Q12125; 13.
DR MINT; Q12125; -.
DR STRING; 4932.YOR164C; -.
DR TCDB; 3.A.21.1.1; the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.
DR iPTMnet; Q12125; -.
DR MaxQB; Q12125; -.
DR PaxDb; Q12125; -.
DR PRIDE; Q12125; -.
DR EnsemblFungi; YOR164C_mRNA; YOR164C; YOR164C.
DR GeneID; 854335; -.
DR KEGG; sce:YOR164C; -.
DR SGD; S000005690; GET4.
DR VEuPathDB; FungiDB:YOR164C; -.
DR eggNOG; KOG3024; Eukaryota.
DR GeneTree; ENSGT00390000015750; -.
DR HOGENOM; CLU_046061_0_2_1; -.
DR InParanoid; Q12125; -.
DR OMA; QIGEMYF; -.
DR BioCyc; YEAST:G3O-33680-MON; -.
DR EvolutionaryTrace; Q12125; -.
DR PRO; PR:Q12125; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12125; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0072380; C:TRC complex; IDA:SGD.
DR GO; GO:0006620; P:post-translational protein targeting to endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:0045048; P:protein insertion into ER membrane; HGI:SGD.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR007317; GET4.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR12875; PTHR12875; 1.
DR Pfam; PF04190; GET4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; ER-Golgi transport; Reference proteome; Transport.
FT CHAIN 1..312
FT /note="Golgi to ER traffic protein 4"
FT /id="PRO_0000228107"
FT HELIX 13..26
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 29..45
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 49..65
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 69..85
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 92..102
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 112..125
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 134..146
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 150..158
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 162..178
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 184..200
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 204..222
FT /evidence="ECO:0007829|PDB:2WPV"
FT STRAND 226..231
FT /evidence="ECO:0007829|PDB:2WPV"
FT STRAND 234..241
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 243..257
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 260..269
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 271..276
FT /evidence="ECO:0007829|PDB:2WPV"
FT HELIX 278..289
FT /evidence="ECO:0007829|PDB:2WPV"
SQ SEQUENCE 312 AA; 36280 MW; 98AFFDB4EA51B40F CRC64;
MVPAESNAVQ AKLAKTLQRF ENKIKAGDYY EAHQTLRTIA NRYVRSKSYE HAIELISQGA
LSFLKAKQGG SGTDLIFYLL EVYDLAEVKV DDISVARLVR LIAELDPSEP NLKDVITGMN
NWSIKFSEYK FGDPYLHNTI GSKLLEGDFV YEAERYFMLG THDSMIKYVD LLWDWLCQVD
DIEDSTVAEF FSRLVFNYLF ISNISFAHES KDIFLERFIE KFHPKYEKID KNGYEIVFFE
DYSDLNFLQL LLITCQTKDK SYFLNLKNHY LDFSQAYKSE LEFLGQEYFN IVAPKQTNFL
QDMMSGFLGG SK
