GEX1_ARATH
ID GEX1_ARATH Reviewed; 593 AA.
AC Q681K7; Q49L63; Q9FJ61;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Protein GAMETE EXPRESSED 1;
DE Flags: Precursor;
GN Name=GEX1; OrderedLocusNames=At5g55490; ORFNames=MTE17.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-586, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Pollen;
RX PubMed=16055690; DOI=10.1104/pp.104.054213;
RA Engel M.L., Holmes-Davis R., McCormick S.;
RT "Green sperm. Identification of male gamete promoters in Arabidopsis.";
RL Plant Physiol. 138:2124-2133(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 57-593.
RA Cheuk R., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, SUBUNIT, DOMAIN, AND DISRUPTION PHENOTYPE.
RX PubMed=21831199; DOI=10.1111/j.1365-313x.2011.04713.x;
RA Alandete-Saez M., Ron M., Leiboff S., McCormick S.;
RT "Arabidopsis thaliana GEX1 has dual functions in gametophyte development
RT and early embryogenesis.";
RL Plant J. 68:620-632(2011).
CC -!- FUNCTION: Has a dual function during gametophyte development and early
CC embryogenesis. Required for correct pollen maturation.
CC {ECO:0000269|PubMed:21831199}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:21831199}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16055690};
CC Multi-pass membrane protein {ECO:0000269|PubMed:16055690}.
CC -!- TISSUE SPECIFICITY: In tricellular pollen, expressed in mature sperm
CC cells. Not expressed in bicellular or unicellular pollen. Detected in
CC ovules, roots and guard cells. Expressed in the embryo sac before
CC cellularization, in the egg cell after cellularization, in the
CC zygote/embryo immediately after fertilization and in the pollen
CC vegetative cell. {ECO:0000269|PubMed:16055690,
CC ECO:0000269|PubMed:21831199}.
CC -!- DOMAIN: The extracellular domain (25-427) is sufficient for male and
CC female gametophyte development before double fertilization, but not for
CC early embryogenesis. The cytoplasmic domain (507-593) is necessary for
CC correct early embryogenesis and mediates homodimer formation at the
CC plasma membrane. {ECO:0000269|PubMed:21831199}.
CC -!- DISRUPTION PHENOTYPE: Embryo lethal when homozygote.
CC {ECO:0000269|PubMed:21831199}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08566.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB015479; BAB08566.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96634.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68230.1; -; Genomic_DNA.
DR EMBL; AK175610; BAD43373.1; -; mRNA.
DR EMBL; AY746360; AAW70162.1; -; mRNA.
DR EMBL; BT012292; AAS76779.1; -; mRNA.
DR RefSeq; NP_001318802.1; NM_001345127.1.
DR RefSeq; NP_200360.2; NM_124931.2.
DR AlphaFoldDB; Q681K7; -.
DR SMR; Q681K7; -.
DR STRING; 3702.AT5G55490.1; -.
DR PaxDb; Q681K7; -.
DR PRIDE; Q681K7; -.
DR ProteomicsDB; 247131; -.
DR EnsemblPlants; AT5G55490.1; AT5G55490.1; AT5G55490.
DR EnsemblPlants; AT5G55490.9; AT5G55490.9; AT5G55490.
DR GeneID; 835642; -.
DR Gramene; AT5G55490.1; AT5G55490.1; AT5G55490.
DR Gramene; AT5G55490.9; AT5G55490.9; AT5G55490.
DR KEGG; ath:AT5G55490; -.
DR Araport; AT5G55490; -.
DR TAIR; locus:2173877; AT5G55490.
DR eggNOG; ENOG502QR0N; Eukaryota.
DR HOGENOM; CLU_032432_0_0_1; -.
DR InParanoid; Q681K7; -.
DR OrthoDB; 166859at2759; -.
DR PhylomeDB; Q681K7; -.
DR PRO; PR:Q681K7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q681K7; baseline and differential.
DR Genevisible; Q681K7; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IDA:UniProtKB.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:UniProtKB.
DR GO; GO:0009553; P:embryo sac development; IMP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR InterPro; IPR040346; GEX1/Brambleberry.
DR PANTHER; PTHR33538; PTHR33538; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Coiled coil; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..593
FT /note="Protein GAMETE EXPRESSED 1"
FT /id="PRO_0000416784"
FT TOPO_DOM 25..427
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..448
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 449..457
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..476
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 477..485
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 486..506
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 507..593
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT COILED 156..194
FT /evidence="ECO:0000255"
FT COILED 350..387
FT /evidence="ECO:0000255"
FT CONFLICT 360
FT /note="Q -> P (in Ref. 4; AAW70162)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 593 AA; 67963 MW; C45A1E305703223B CRC64;
MDRFSRKCLL FLLLIILLDS PLTCHSWGWF SSSSSSAEDP YSSSFSRSRK SNPDFSMEVF
SDQKAVQVLE NKLVGLTSCW QNAYSYLLAG CKETIATEEK RKRFAWYLSD CFIKDSGRPA
FPTCKDESVM MSCLKKLDDH EHKIYLDFLL ETNTICQQLQ SNAFKNEIER LVNELKNTAQ
YTEDKLDILE SKSDALIQTS SMIHDSLGSL DVRVQNVASV TNTLETSVSG LSQQTVEISQ
EQKNIAESQL ALRDGQVKMK ETLKDGMDMF LDAYTNIQEG VDKLKSDTEQ IEVEISVLGN
NLSTKMIDLQ STTDDIGTKT RSSLDKQQKL LDGQTVALDG IQFLTRFQSE ALQESRNTLQ
RLKEFSQEQQ EDLAKRQEKL QEVHDHLFEN SKSMLEAQVA FEAKQANMFV ALDKLFALHN
AMLLESRVIK AFVIYFLSIF VIYMFTSTKQ TYIIRPRLYI GLCVTLALEV ASLRYVNDTE
RQAWMINLIR SLFALLASAQ LLHAALSYRD YEVLNHQILL RLVDKVNDMQ SKKELSYDED
TESEVDWTSW VDTDLTDDDD NLADPDYKIP LLIKDNPVTT SSLTRRLYNF RPR
