GEX2_ARATH
ID GEX2_ARATH Reviewed; 914 AA.
AC F4K4R6; Q49L64; Q9FJ23;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Protein GAMETE EXPRESSED 2;
GN Name=GEX2; OrderedLocusNames=At5g49150; ORFNames=K21P3.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 30-914, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Pollen;
RX PubMed=16055690; DOI=10.1104/pp.104.054213;
RA Engel M.L., Holmes-Davis R., McCormick S.;
RT "Green sperm. Identification of male gamete promoters in Arabidopsis.";
RL Plant Physiol. 138:2124-2133(2005).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=19825564; DOI=10.1093/mp/ssn015;
RA Alandete-Saez M., Ron M., McCormick S.;
RT "GEX3, expressed in the male gametophyte and in the egg cell of Arabidopsis
RT thaliana, is essential for micropylar pollen tube guidance and plays a role
RT during early embryogenesis.";
RL Mol. Plant 1:586-598(2008).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16055690};
CC Single-pass membrane protein {ECO:0000269|PubMed:16055690}.
CC -!- TISSUE SPECIFICITY: In tricellular pollen, expressed in mature sperm
CC cells but not in the vegetative cell. In bicellular pollen, detected in
CC the progenitor generative cell. Detected in the egg cell within the
CC female gametophyte. {ECO:0000269|PubMed:16055690,
CC ECO:0000269|PubMed:19825564}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10333.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB016872; BAB10333.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95776.1; -; Genomic_DNA.
DR EMBL; AY746359; AAW70161.1; -; mRNA.
DR RefSeq; NP_199726.3; NM_124292.3.
DR AlphaFoldDB; F4K4R6; -.
DR STRING; 3702.AT5G49150.1; -.
DR PaxDb; F4K4R6; -.
DR ProteomicsDB; 220734; -.
DR EnsemblPlants; AT5G49150.1; AT5G49150.1; AT5G49150.
DR GeneID; 834974; -.
DR Gramene; AT5G49150.1; AT5G49150.1; AT5G49150.
DR KEGG; ath:AT5G49150; -.
DR Araport; AT5G49150; -.
DR TAIR; locus:2155949; AT5G49150.
DR eggNOG; ENOG502QWD1; Eukaryota.
DR HOGENOM; CLU_009992_0_0_1; -.
DR InParanoid; F4K4R6; -.
DR PRO; PR:F4K4R6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4K4R6; differential.
DR Genevisible; F4K4R6; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:TAIR.
DR GO; GO:0051015; F:actin filament binding; IEA:InterPro.
DR GO; GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
DR GO; GO:0061936; P:fusion of sperm to egg plasma membrane involved in double fertilization forming a zygote and endosperm; IMP:TAIR.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IEP:TAIR.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR044801; Filamin.
DR InterPro; IPR017868; Filamin/ABP280_repeat-like.
DR InterPro; IPR001298; Filamin/ABP280_rpt.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR38537; PTHR38537; 1.
DR Pfam; PF00630; Filamin; 2.
DR SMART; SM00557; IG_FLMN; 1.
DR SUPFAM; SSF81296; SSF81296; 2.
DR PROSITE; PS50194; FILAMIN_REPEAT; 2.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..914
FT /note="Protein GAMETE EXPRESSED 2"
FT /id="PRO_0000416785"
FT TRANSMEM 893..913
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 249..382
FT /note="Filamin 1"
FT REPEAT 391..485
FT /note="Filamin 2"
FT CONFLICT 320
FT /note="T -> A (in Ref. 3; AAW70161)"
FT /evidence="ECO:0000305"
FT CONFLICT 841
FT /note="V -> A (in Ref. 3; AAW70161)"
FT /evidence="ECO:0000305"
FT CONFLICT 893
FT /note="L -> S (in Ref. 3; AAW70161)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 914 AA; 100588 MW; 3B493B107760A4D7 CRC64;
MVGIFNIVID EENFKVLDSS LHFEVEAGLM YPSVSVVSWM GLANVFEAGM NASILILPKD
AFGNNISFSG KKMEFQEFSL SLISENGSFA GVLNSTHIRW IVSGYISIDF VLVTSGKFLL
LVEKESQTLN GGPLPLEVNS GPLDVSNCVS IWKSELSTWQ IFSKMEILLH QKDRFGNIVS
GFYEFDADVV EVETGLSIPV ADFQFEYVEP GIQLMSFTLS EPGNFLLTLS DMKHNKSISS
MPYVYTVYIG YCDGSRSIVN GSGINASIAG ESLGFSVYLK DAYGYPSPVQ VDRLQVRIVL
EIDSSIILPT IQPREALNGT GSSHQAATPL YEKHGGRASG NLVTQASIFD VTYTPKRTGI
YRIFISSGNI VLNGGQPFIK EVYAGEVNVA ACSVTQFNGK VPKEIKNEIV VLLLDGFYNP
VPSQPSRLKF EITSANTSSF TTWEFVDNND GTYTGSYLAM EVGTYRMCIS FDNKHIQPCP
FDVNVYSNGY FPRAYDDPVN VWEDESISFN PLENDYFAGD NASMLGFSQP GHGSLLRDGN
LLRYTPMKNF SGNDSFLYTI ADINGNLAAA TVYIFVLTAP PQFVSFSGGL QATEDLISPR
YGGFSGLEIS YSDLLENISV MVQALSGSVI LSPMLMQFRP PGSGKLSVSN GGEDRRVLIL
EGQVGVINPA LQSIQYLGNE NFAGVDSLRL STKNKNGINH LDVPVFVEPV NDPPFINVPQ
YIMLESNGSE SLIFHPERDK FNFSVGDPDL VNFPGGESHF LVTFSLEVTD GFLLTNLPSE
LINSTELKFK NLFQWQPIQT YAAISKHVNV KASGIRFRGT IRQCNDLMQQ LLHRGGENGA
VLTLKLSDMG NYGCFLDCTE RISLPLHAEA RVNLIRKRPL SSLGAHGTFM KYLVVVPFSF
FSIKLFSLLM VLIG
