GEX3_ARATH
ID GEX3_ARATH Reviewed; 641 AA.
AC Q9LFS2;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Protein GAMETE EXPRESSED 3;
DE Flags: Precursor;
GN Name=GEX3; OrderedLocusNames=At5g16020; ORFNames=F1N13.160;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=19825564; DOI=10.1093/mp/ssn015;
RA Alandete-Saez M., Ron M., McCormick S.;
RT "GEX3, expressed in the male gametophyte and in the egg cell of Arabidopsis
RT thaliana, is essential for micropylar pollen tube guidance and plays a role
RT during early embryogenesis.";
RL Mol. Plant 1:586-598(2008).
CC -!- FUNCTION: Required for micropylar pollen tube guidance. Plays a role
CC during early embryo patterning. {ECO:0000269|PubMed:19825564}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19825564};
CC Single-pass membrane protein {ECO:0000269|PubMed:19825564}.
CC -!- TISSUE SPECIFICITY: Expressed in mature siliques and in pollen, mainly
CC in the sperm cells. Detected in the egg cell within the female
CC gametophyte. {ECO:0000269|PubMed:19825564}.
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DR EMBL; AL391145; CAC01801.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92235.1; -; Genomic_DNA.
DR PIR; T51385; T51385.
DR RefSeq; NP_197106.1; NM_121607.2.
DR AlphaFoldDB; Q9LFS2; -.
DR SMR; Q9LFS2; -.
DR BioGRID; 16735; 1.
DR STRING; 3702.AT5G16020.1; -.
DR PaxDb; Q9LFS2; -.
DR PRIDE; Q9LFS2; -.
DR ProteomicsDB; 220735; -.
DR EnsemblPlants; AT5G16020.1; AT5G16020.1; AT5G16020.
DR GeneID; 831459; -.
DR Gramene; AT5G16020.1; AT5G16020.1; AT5G16020.
DR KEGG; ath:AT5G16020; -.
DR Araport; AT5G16020; -.
DR TAIR; locus:2146122; AT5G16020.
DR eggNOG; ENOG502QSCU; Eukaryota.
DR HOGENOM; CLU_026343_1_0_1; -.
DR InParanoid; Q9LFS2; -.
DR OMA; KHDSILP; -.
DR OrthoDB; 714695at2759; -.
DR PhylomeDB; Q9LFS2; -.
DR PRO; PR:Q9LFS2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LFS2; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0099503; C:secretory vesicle; HDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0010183; P:pollen tube guidance; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR045301; GEX3-like.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR PANTHER; PTHR37253; PTHR37253; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..641
FT /note="Protein GAMETE EXPRESSED 3"
FT /id="PRO_0000416786"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 570..627
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 641 AA; 73340 MW; 322B0A0CED11A8AB CRC64;
MVAFRFVYIP LPFFFFFFFF FVFFSGVSQL QDQTATKKSV RILSKILIGD DGRVYACSDN
DFFSFESNGS IAWSVHMNFK CNTDFAPVYS GFNQMLLLAE NRILRVIFPR NGTKSEPELF
FDPGETILGF AVSVSSSSVY ITVKNHGLYA YNMFRQQLWI AEPKIERFGY RLGCRKDFDN
CTFNSRPVID SCEGSIYISN NEGELYSLSL RGTYYQWIQD FSLVDRFFTV TPGNNGLVYV
VFPIKSLVFA LDSFSGDILW QKTIGPLAET SASDPVIDSN SWASIGSLDG TLYSFSRTGD
LYKIPKNAET DSVIQIEPLL DCSGYAVYVS QTKFEGMIDR VIEDYTYVSA KKPETAVFSL
VVPETRSIYW SQSYSDQIPG LLLDEDLQHF VLDERIALAF VAASSSGNPF RCRSKHEKLS
SSCSFAEPEH LDIYIGNERA IIWFLLFEFV IMVLFAALVR FCFIFWKKKK LQDRPFSTFL
DKRRLLHRKS REIDKTITRL QNESTANESA VDKIGDLIQK RENVKRKLSS TYSLGRDIDE
SKSKLKDYVL PLYGGSSRSF SYRNRENESI TIFQTPSDES SSEESYRDEH YDDVADDEHD
EDDLDRKQKG KLLAHSEGSS NDGDGIASSR RSIYLKHIFD D
