ID   GFCA_ECOLI              Reviewed;         101 AA.
AC   P75885;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 2.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Threonine-rich inner membrane protein GfcA;
DE   AltName: Full=Group 4 capsule protein A homolog;
DE   Flags: Precursor;
GN   Name=gfcA; Synonyms=ymcD; OrderedLocusNames=b0987, JW5133;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Single-pass membrane
CC       protein. Note=When the protein is overexpressed.
CC   -!- CAUTION: In E.coli K12 / MG1655 and K12 / W3110 this operon is silenced
CC       by an IS1D insertion in the promoter region. {ECO:0000305}.
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DR   EMBL; U00096; AAC74072.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35752.1; -; Genomic_DNA.
DR   PIR; A64840; A64840.
DR   RefSeq; NP_415507.2; NC_000913.3.
DR   RefSeq; WP_001295358.1; NZ_SSZK01000002.1.
DR   AlphaFoldDB; P75885; -.
DR   BioGRID; 4262044; 9.
DR   DIP; DIP-12710N; -.
DR   IntAct; P75885; 2.
DR   STRING; 511145.b0987; -.
DR   PaxDb; P75885; -.
DR   EnsemblBacteria; AAC74072; AAC74072; b0987.
DR   EnsemblBacteria; BAA35752; BAA35752; BAA35752.
DR   GeneID; 66670736; -.
DR   GeneID; 945029; -.
DR   KEGG; ecj:JW5133; -.
DR   KEGG; eco:b0987; -.
DR   PATRIC; fig|511145.12.peg.1022; -.
DR   EchoBASE; EB3496; -.
DR   eggNOG; ENOG5033YAD; Bacteria.
DR   OMA; GHDSNPG; -.
DR   BioCyc; EcoCyc:G6508-MON; -.
DR   PRO; PR:P75885; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..101
FT                   /note="Threonine-rich inner membrane protein GfcA"
FT                   /id="PRO_0000168799"
FT   TOPO_DOM        22..59
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        81..101
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          24..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          82..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        26..45
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        86..101
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   101 AA;  9508 MW;  AE3E86C5E6166C83 CRC64;
     MKHKLSAILM AFMLTTPAAF AAPEATNGTE ATTGTTGTTT TTTGATTTAT TTGGVAAGAV
     GTATVVGVAT AVGVATLAVV AANDSGDGGS HNTSTTTSTT R