GFCB_ECOLI
ID GFCB_ECOLI Reviewed; 214 AA.
AC P75884;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Uncharacterized lipoprotein GfcB;
DE AltName: Full=Group 4 capsule protein B homolog;
DE Flags: Precursor;
GN Name=gfcB; Synonyms=ymcC; OrderedLocusNames=b0986, JW0969;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 17-214.
RG Northeast structural genomics consortium (NESG);
RT "Crystal structure of the hypothetical lipoprotein ymcC from Escherichia
RT coli (K12), Northeast structural genomics target ER552.";
RL Submitted (NOV-2006) to the PDB data bank.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC -!- SIMILARITY: To E.coli YjbF. {ECO:0000305}.
CC -!- CAUTION: In E.coli K12 / MG1655 and K12 / W3110 this operon is silenced
CC by an IS1D insertion in the promoter region. {ECO:0000305}.
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DR EMBL; U00096; AAC74071.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35751.1; -; Genomic_DNA.
DR PIR; H64839; H64839.
DR RefSeq; NP_415506.1; NC_000913.3.
DR RefSeq; WP_001247610.1; NZ_STEB01000006.1.
DR PDB; 2IN5; X-ray; 2.30 A; A/B=17-214.
DR PDBsum; 2IN5; -.
DR AlphaFoldDB; P75884; -.
DR SMR; P75884; -.
DR DIP; DIP-12709N; -.
DR IntAct; P75884; 1.
DR STRING; 511145.b0986; -.
DR PaxDb; P75884; -.
DR PRIDE; P75884; -.
DR EnsemblBacteria; AAC74071; AAC74071; b0986.
DR EnsemblBacteria; BAA35751; BAA35751; BAA35751.
DR GeneID; 66670737; -.
DR GeneID; 949118; -.
DR KEGG; ecj:JW0969; -.
DR KEGG; eco:b0986; -.
DR PATRIC; fig|1411691.4.peg.1287; -.
DR EchoBASE; EB3495; -.
DR eggNOG; ENOG502ZAMG; Bacteria.
DR HOGENOM; CLU_098990_1_0_6; -.
DR OMA; AISAIWQ; -.
DR PhylomeDB; P75884; -.
DR BioCyc; EcoCyc:G6507-MON; -.
DR EvolutionaryTrace; P75884; -.
DR PRO; PR:P75884; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.40.360.10; -; 1.
DR InterPro; IPR021308; GfcB.
DR InterPro; IPR023373; YmcC_sf.
DR Pfam; PF11102; YjbF; 1.
DR SUPFAM; SSF159270; SSF159270; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Signal.
FT SIGNAL 1..15
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 16..214
FT /note="Uncharacterized lipoprotein GfcB"
FT /id="PRO_0000018055"
FT LIPID 16
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 16
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT HELIX 23..33
FT /evidence="ECO:0007829|PDB:2IN5"
FT HELIX 41..46
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 62..70
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 73..77
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 83..87
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 90..99
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 101..106
FT /evidence="ECO:0007829|PDB:2IN5"
FT HELIX 107..109
FT /evidence="ECO:0007829|PDB:2IN5"
FT HELIX 111..117
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 123..132
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 135..155
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 158..171
FT /evidence="ECO:0007829|PDB:2IN5"
FT TURN 172..175
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 176..185
FT /evidence="ECO:0007829|PDB:2IN5"
FT TURN 186..188
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 191..201
FT /evidence="ECO:0007829|PDB:2IN5"
FT STRAND 203..209
FT /evidence="ECO:0007829|PDB:2IN5"
SQ SEQUENCE 214 AA; 24268 MW; 77A3111CBAF3E90D CRC64;
MRPLILSIFA LFLAGCTHSQ QSMVDTFRAS LFDNQDITVA DQQIQALPYS TMYLRLNEGQ
RIFVVLGYIE QEQSKWLSQD NAMLVTHNGR LLKTVKLNNN LLEVTNSGQD PLRNALAIKD
GSRWTRDILW SEDNHFRSAT LSSTFSFAGL ETLNIAGRNV LCNVWQEEVT STRPEKQWQN
TFWVDSATGQ VRQSRQMLGA GVIPVEMTFL KPAP
