GFH1_SCHPO
ID GFH1_SCHPO Reviewed; 577 AA.
AC Q9P7R5;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Gamma-tubulin complex component gfh1;
DE AltName: Full=Gcp4 homolog 1;
GN Name=gfh1; ORFNames=SPBC211.06;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15004232; DOI=10.1091/mbc.e03-10-0728;
RA Venkatram S., Tasto J.J., Feoktistova A., Jennings J.L., Link A.J.,
RA Gould K.L.;
RT "Identification and characterization of two novel proteins affecting
RT fission yeast gamma-tubulin complex function.";
RL Mol. Biol. Cell 15:2287-2301(2004).
CC -!- FUNCTION: Required for proper anchoring of astral microtubules at the
CC spindle pole bodies (SPBs), during anaphase, ensuring correct cell
CC polarity. {ECO:0000269|PubMed:15004232}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, spindle pole body {ECO:0000269|PubMed:15004232,
CC ECO:0000269|PubMed:16823372}. Note=Localizes at the microtubule
CC organizing center (MTOC).
CC -!- SIMILARITY: Belongs to the TUBGCP family. {ECO:0000305}.
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DR EMBL; CU329671; CAB75414.1; -; Genomic_DNA.
DR PIR; T50341; T50341.
DR RefSeq; NP_596616.1; NM_001022537.2.
DR AlphaFoldDB; Q9P7R5; -.
DR SMR; Q9P7R5; -.
DR BioGRID; 277192; 25.
DR STRING; 4896.SPBC211.06.1; -.
DR PaxDb; Q9P7R5; -.
DR EnsemblFungi; SPBC211.06.1; SPBC211.06.1:pep; SPBC211.06.
DR GeneID; 2540667; -.
DR KEGG; spo:SPBC211.06; -.
DR PomBase; SPBC211.06; gfh1.
DR VEuPathDB; FungiDB:SPBC211.06; -.
DR HOGENOM; CLU_482466_0_0_1; -.
DR InParanoid; Q9P7R5; -.
DR OMA; YFYDGII; -.
DR PRO; PR:Q9P7R5; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000923; C:equatorial microtubule organizing center; IDA:PomBase.
DR GO; GO:0000930; C:gamma-tubulin complex; IDA:PomBase.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0044732; C:mitotic spindle pole body; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0000922; C:spindle pole; IEA:InterPro.
DR GO; GO:0043015; F:gamma-tubulin binding; IBA:GO_Central.
DR GO; GO:0030953; P:astral microtubule organization; IMP:PomBase.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; IBA:GO_Central.
DR GO; GO:0051415; P:microtubule nucleation by interphase microtubule organizing center; IGI:PomBase.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR Gene3D; 1.20.120.1900; -; 1.
DR InterPro; IPR007259; GCP.
DR InterPro; IPR040457; GCP_C.
DR InterPro; IPR042241; GCP_C_sf.
DR InterPro; IPR041470; GCP_N.
DR PANTHER; PTHR19302; PTHR19302; 1.
DR Pfam; PF04130; GCP_C_terminal; 1.
DR Pfam; PF17681; GCP_N_terminal; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cytoplasm; Cytoskeleton; Microtubule; Reference proteome.
FT CHAIN 1..577
FT /note="Gamma-tubulin complex component gfh1"
FT /id="PRO_0000362147"
SQ SEQUENCE 577 AA; 66765 MW; 206ECC12E9000303 CRC64;
MLHEIILLLA GNTSGLFIEK NGQILLHPSV QPLHPGEREL INDIVSLASK RKRIERYIEI
FDEDLIQQNQ FLLKALRLLL SKKLFLYDES LANLERMIIS SDPYFVGSGK FVSLAQLEAH
LADWKETLSS LLVLQGNISQ CLSVASTFQK LHNMYMGSTH LNFKKLVLEC ECAFQKTWLD
ELVQYLVNDS GDDSFKCSFL CGDTKDFVSC SNNVPFLTQD IVNCLVSIRT CMLNAKKCFT
DAFCEILRCF YRLVYEMPLP LTKSSVTKLA DEMFTLVSEK IIFQLASFHQ IFDLVKMLEM
VMLLENVEFL PTLSEMFRDV RGEYCSSNDV QGVVIDDILP VVLRKTISLL DLATEYGECV
SFLKLVSIFD EEDEMIRSEN RKIDVEGLNM QLLLCLKWPF NLFMDKNALN VYSDLWILLG
SLHLSITKIK SLFYERKEYT AKGISQPWTQ LWVTLWFLSS LQYYAYECVI KPSYCKLRES
LTELYRTQKL RMQDCSQLHA LTLTYAKKML FFYDDDLHIS LNSIYHELSL FRSQEVSKVD
CQLAAHVSHS IECIRQKEND PSYDREIINA LIVQLNL
