ID   GFI1B_CHICK             Reviewed;         337 AA.
AC   O42409;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Zinc finger protein Gfi-1b;
DE   AltName: Full=Growth factor-independent protein 1B;
GN   Name=GFI1B; Synonyms=GFI;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=9305773; DOI=10.1016/s0378-1119(97)00175-3;
RA   Fuchs B., Wagner T., Roessel N., Antoine M., Beug H., Niessing J.;
RT   "Structure and erythroid cell-restricted expression of a chicken cDNA
RT   encoding a novel zinc finger protein of the Cys + His class.";
RL   Gene 195:277-284(1997).
CC   -!- FUNCTION: Essential transcriptional regulator necessary for development
CC       and differentiation of erythroid and megakaryocytic lineages. Alters
CC       histone methylation by recruiting histone methyltransferase to target
CC       genes promoters. Plays a role in heterochromatin formation.
CC       {ECO:0000269|PubMed:9305773}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in erythroid cells of primitive and
CC       definitive lineage and bone marrow cells. {ECO:0000269|PubMed:9305773}.
CC   -!- DOMAIN: The zinc finger domain is essential for erythroid expansion and
CC       acts as an activation domain whereas non finger domain serves as
CC       repression domain. {ECO:0000250}.
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DR   EMBL; Y10898; CAA71836.1; -; mRNA.
DR   RefSeq; NP_990422.1; NM_205091.1.
DR   RefSeq; XP_015134821.1; XM_015279335.1.
DR   AlphaFoldDB; O42409; -.
DR   SMR; O42409; -.
DR   STRING; 9031.ENSGALP00000005496; -.
DR   PaxDb; O42409; -.
DR   Ensembl; ENSGALT00000005506; ENSGALP00000005496; ENSGALG00000003478.
DR   GeneID; 395977; -.
DR   KEGG; gga:395977; -.
DR   CTD; 8328; -.
DR   VEuPathDB; HostDB:geneid_395977; -.
DR   eggNOG; KOG1721; Eukaryota.
DR   GeneTree; ENSGT00940000160010; -.
DR   HOGENOM; CLU_002678_94_9_1; -.
DR   InParanoid; O42409; -.
DR   OMA; HTYHQHR; -.
DR   OrthoDB; 1318335at2759; -.
DR   PhylomeDB; O42409; -.
DR   TreeFam; TF350784; -.
DR   PRO; PR:O42409; -.
DR   Proteomes; UP000000539; Chromosome 17.
DR   Bgee; ENSGALG00000003478; Expressed in lung and 9 other tissues.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF00096; zf-C2H2; 5.
DR   SMART; SM00355; ZnF_C2H2; 6.
DR   SUPFAM; SSF57667; SSF57667; 3.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 6.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 6.
PE   2: Evidence at transcript level;
KW   Chromatin regulator; Developmental protein; DNA-binding; Metal-binding;
KW   Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..337
FT                   /note="Zinc finger protein Gfi-1b"
FT                   /id="PRO_0000306329"
FT   ZN_FING         170..193
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         199..221
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         227..249
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         255..277
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         283..305
FT                   /note="C2H2-type 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         311..334
FT                   /note="C2H2-type 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          1..20
FT                   /note="Mediates repression of transcription"
FT                   /evidence="ECO:0000250"
FT   REGION          1..20
FT                   /note="SNAG domain"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   337 AA;  38687 MW;  55871DA1D1A4F4FB CRC64;
     MPRSFLVKSK KAHTYHQHRF VEDDLPIFTW DPITSAFTAA GDRDKTPEDG KKQDLEWVVP
     KQEKDSFQPK EESVPVQYLS RMLPGPSAQD MSISGLQVKD CTNPTSMPTF YKTGFSWDAF
     QLPYSYRQMS STMQSALLEH PVSLYGSHLL PSAEPPLDYS MRYSSDMETY HCVKCNKVFS
     TPHGLEVHVR RSHSGTRPFA CEVCGKTFGH AVSLEQHTNI HSQERSFECK MCGKTFKRSS
     TLSTHLLIHS DTRPYPCQYC GKRFHQKSDM KKHTYIHTGE KPHKCQVCGK AFSQSSNLIT
     HSRKHTGFKP FSCELCAKGF QRKVDLRRHR ETQHSLK