GFI3_CAEEL
ID GFI3_CAEEL Reviewed; 800 AA.
AC Q93903;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Protein gfi-3 {ECO:0000312|EMBL:CAB01894.1};
GN Name=gfi-3 {ECO:0000312|WormBase:M163.4};
GN ORFNames=M163.4 {ECO:0000312|WormBase:M163.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP DISRUPTION PHENOTYPE.
RX PubMed=11861581; DOI=10.1093/genetics/160.2.805;
RA Davis E.S., Wille L., Chestnut B.A., Sadler P.L., Shakes D.C., Golden A.;
RT "Multiple subunits of the Caenorhabditis elegans anaphase-promoting complex
RT are required for chromosome segregation during meiosis I.";
RL Genetics 160:805-813(2002).
RN [3] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=20944012; DOI=10.1534/genetics.110.123463;
RA Stein K.K., Nesmith J.E., Ross B.D., Golden A.;
RT "Functional redundancy of paralogs of an anaphase promoting
RT complex/cyclosome subunit in Caenorhabditis elegans meiosis.";
RL Genetics 186:1285-1293(2010).
CC -!- FUNCTION: Probable component of the anaphase promoting
CC complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle. The APC/C complex acts by mediating ubiquitination and
CC subsequent degradation of target proteins. Required for the metaphase
CC to anaphase transition in meiosis. {ECO:0000269|PubMed:20944012}.
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBUNIT: The APC/C complex is probably composed of at least 12
CC subunits: apc-2, apc-10, apc-11, cdc-26, emb-1, emb-27, emb-30, mat-1,
CC mat-2, mat-3, such-1 and gfi-3. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in gut cells and mature sperm stored in
CC the spermatheca. {ECO:0000269|PubMed:20944012}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing germ line and
CC throughout embryogenesis and the L1-L4 larval stages.
CC {ECO:0000269|PubMed:20944012}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in both viable
CC and inviable embryos, but eventually animals display germline
CC maintenance defects and become sterile (PubMed:11861581). Double RNAi-
CC mediated knockdown and/or mutagenesis with such-1 results in increased
CC embryonic lethality and the production of one-cell arrested embryos
CC (PubMed:20944012). {ECO:0000269|PubMed:11861581,
CC ECO:0000269|PubMed:20944012}.
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DR EMBL; Z79603; CAB01894.1; -; Genomic_DNA.
DR PIR; T23780; T23780.
DR RefSeq; NP_510409.1; NM_078008.4.
DR AlphaFoldDB; Q93903; -.
DR SMR; Q93903; -.
DR ComplexPortal; CPX-3382; Anaphase Promoting Complex/Cyclosome.
DR IntAct; Q93903; 1.
DR STRING; 6239.M163.4; -.
DR EPD; Q93903; -.
DR PaxDb; Q93903; -.
DR EnsemblMetazoa; M163.4.1; M163.4.1; WBGene00001583.
DR GeneID; 181544; -.
DR KEGG; cel:CELE_M163.4; -.
DR UCSC; M163.4; c. elegans.
DR CTD; 181544; -.
DR WormBase; M163.4; CE12452; WBGene00001583; gfi-3.
DR eggNOG; ENOG502S8D6; Eukaryota.
DR GeneTree; ENSGT00390000018674; -.
DR HOGENOM; CLU_351691_0_0_1; -.
DR InParanoid; Q93903; -.
DR OMA; WTAIRIN; -.
DR OrthoDB; 477915at2759; -.
DR PhylomeDB; Q93903; -.
DR Reactome; R-CEL-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q93903; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001583; Expressed in embryo and 4 other tissues.
DR GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0045842; P:positive regulation of mitotic metaphase/anaphase transition; IBA:GO_Central.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; IBA:GO_Central.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; IC:ComplexPortal.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IC:ComplexPortal.
DR GO; GO:1904666; P:regulation of ubiquitin protein ligase activity; IC:ComplexPortal.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR037679; Apc5.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR12830; PTHR12830; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50005; TPR; 2.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Coiled coil; Developmental protein; Meiosis;
KW Mitosis; Reference proteome; Repeat; TPR repeat; Ubl conjugation pathway.
FT CHAIN 1..800
FT /note="Protein gfi-3"
FT /evidence="ECO:0000305"
FT /id="PRO_0000435337"
FT REPEAT 526..559
FT /note="TPR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 637..670
FT /note="TPR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT COILED 346..366
FT /evidence="ECO:0000255"
SQ SEQUENCE 800 AA; 91511 MW; 5830CC9338AFA0CC CRC64;
MQKAAPRPII KRSYLNLDAI FGHVFSNLTG EPVTPTKIAT FYLLRILFQT HFGFRHKRAY
YKPFSPAEKE RIFHWLYALM TSVVELSYKD FRIVTRLAFD DGREVCKNFY AAMEKIGAGM
ADVTLNVEDE FYTNKHPKDP KLPANDTVHE AMDLARGDII QFSSNHSFMY RWMKRLLAQY
TKSTPSEVFQ INAAMKSWVL SAVESKYHPE FKGEYLPHFI DCSVRARDWV AKSIYFIQKN
PRFAFQYNEM LHYTRIVQKR HPDVVEGFLL EAIVQVQLKD SSSAIKAMKS FFELSMFELN
ENMVHAAKTH RLAVPTQTPL MYAPILQARI CRIFGDYQMA RLLLHESLQQ AQLRNDEICH
QMANIEMHTV DILGCRALLE DNNEKTAKHI ENERRVQRKA LLHIDDLHGH TRSGPCCLSS
EEDFELVAEM DSYGKMLMLM KIIAEGNYKL IYDRVAETGI TCPVGNDLGE RGRKVAAYGA
ALISSNMIHN GMYHQAKCAA ENMLANNCRT DETDPFQAPF QAEPWAIGAV NLSYSQAGVG
NYDAALRTIE EMRQNYPEEL SWQSNRHVVI CAAVINFERF LLKNDYKACA SEVLNLAAHS
ELECKLRQAL LLAAAGKERA SVEVLEGLNV VDIRGRIRIH MQTGCIYTAS QEFRKAAREF
ADALSLAENT TLKDIVPMVK RRQATMLMCH GQYVECLKLL KECNEGIEQF GSFTEKACYY
MTAARCHRLL GKDPRMWLKK SRALVRNGQW PAFTKLVLSE IAALHDVKGL MPDENRLSQI
CEQFGKLTAD CPGRCEWLLI
