GFL_ARATH
ID GFL_ARATH Reviewed; 973 AA.
AC F4JWP9;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=109 kDa U5 small nuclear ribonucleoprotein component GFL {ECO:0000305};
DE AltName: Full=Protein GFA1-LIKE {ECO:0000303|Ref.3};
DE AltName: Full=SNU114 homolog {ECO:0000305};
GN Name=GFL {ECO:0000303|Ref.3};
GN OrderedLocusNames=At5g25230 {ECO:0000312|Araport:AT5G25230};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX DOI=10.1007/s00497-007-0046-8;
RA Coury D.A., Zhang C., Ara Ko A., Skaggs M.I., Christensen C.A., Drews G.N.,
RA Feldmann K.A., Yadegari R.;
RT "Segregation distortion in Arabidopsis gametophytic factor 1 (gfa1) mutants
RT is caused by a deficiency of an essential RNA splicing factor.";
RL Sex. Plant Reprod. 20:87-97(2007).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=19261069; DOI=10.1111/j.1744-7909.2008.00783.x;
RA Liu M., Yuan L., Liu N.Y., Shi D.Q., Liu J., Yang W.C.;
RT "GAMETOPHYTIC FACTOR 1, involved in pre-mRNA splicing, is essential for
RT megagametogenesis and embryogenesis in Arabidopsis.";
RL J. Integr. Plant Biol. 51:261-271(2009).
CC -!- FUNCTION: Splicing factor involved in pre-mRNA splicing and component
CC of the spliceosome. {ECO:0000250|UniProtKB:Q9LNC5}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250|UniProtKB:Q9LNC5}.
CC -!- TISSUE SPECIFICITY: Expressed in flower buds, open flowers and
CC siliques. Expressed at low levels in rosettes leaves, cauline leaves
CC and stems. {ECO:0000269|Ref.3}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing embryos until heart stage.
CC {ECO:0000269|PubMed:19261069}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|Ref.3}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family.
CC {ECO:0000255|PROSITE-ProRule:PRU01059}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC006259; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93415.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69055.1; -; Genomic_DNA.
DR RefSeq; NP_001318643.1; NM_001343914.1.
DR RefSeq; NP_197905.1; NM_122432.2.
DR AlphaFoldDB; F4JWP9; -.
DR SMR; F4JWP9; -.
DR STRING; 3702.AT5G25230.1; -.
DR PaxDb; F4JWP9; -.
DR PRIDE; F4JWP9; -.
DR ProteomicsDB; 247132; -.
DR EnsemblPlants; AT5G25230.1; AT5G25230.1; AT5G25230.
DR EnsemblPlants; AT5G25230.2; AT5G25230.2; AT5G25230.
DR GeneID; 832594; -.
DR Gramene; AT5G25230.1; AT5G25230.1; AT5G25230.
DR Gramene; AT5G25230.2; AT5G25230.2; AT5G25230.
DR KEGG; ath:AT5G25230; -.
DR Araport; AT5G25230; -.
DR TAIR; locus:2146950; AT5G25230.
DR eggNOG; KOG0468; Eukaryota.
DR HOGENOM; CLU_002794_11_2_1; -.
DR InParanoid; F4JWP9; -.
DR OMA; WITTINE; -.
DR OrthoDB; 140796at2759; -.
DR PhylomeDB; F4JWP9; -.
DR PRO; PR:F4JWP9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4JWP9; baseline and differential.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0030623; F:U5 snRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd04098; eEF2_C_snRNP; 1.
DR CDD; cd04167; Snu114p; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031950; EFTUD2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR044121; Snu114_GTP-bd.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR005517; Transl_elong_EFG/EF2_IV.
DR InterPro; IPR035655; U5-116kDa_C.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF03764; EFG_IV; 1.
DR Pfam; PF16004; EFTUD2; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SMART; SM00889; EFG_IV; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF54980; SSF54980; 2.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 2: Evidence at transcript level;
KW GTP-binding; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus;
KW Reference proteome; Spliceosome.
FT CHAIN 1..973
FT /note="109 kDa U5 small nuclear ribonucleoprotein component
FT GFL"
FT /id="PRO_0000436562"
FT DOMAIN 122..408
FT /note="tr-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 131..138
FT /note="G1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT REGION 175..179
FT /note="G2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT REGION 201..204
FT /note="G3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT REGION 255..258
FT /note="G4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT REGION 381..383
FT /note="G5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT BINDING 131..138
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 201..205
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 255..258
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
SQ SEQUENCE 973 AA; 108804 MW; 5D16E37B75A438FF CRC64;
MDGSLYGECG NYIGPEIESD RDSDDSVEDE DLQEPGGSNG WITTINENQN IVLPEDKKYY
PIAKEVYGED VETLVMDEDE QSLEQPIIKP VRDIRFEVGV IKDQTTTYVS TLFLIGLMSN
PALVRNVALV GHLQHGKTVF MDMLVEQTHR MSTFNAENDK HMRYTDTRVD EQERNISIKA
VPMSLVLEDS RSKSYLCNIM DTPGNVNFSD EMTASLRLAD GAVFIVDAAQ GVMVNTERAI
RHAIQDHLPI VVVINKVDRL ITELKLPPRD AYYKLRYTIE VINNHISAAS TNAADLPLID
PAAGNVCFAS GTAGWSFTLQ SFARMYAKLH GVAMDVDKFA SRLWGDVYYH PDTRVFNTSP
PVGGGERAFV QFILEPLYKI YSQVIGEHKK SVETTLAELG VTLSNSAYKL NVRPLLRLAC
SSVFGSASGF TDMLVKHIPS PREAAARKVD HSYTGTKDSP IYESMVECDP SGPLMVNVTK
LYPKSDTSVF DVFGRVYSGR LQTGQSVRVL GEGYSPEDEE DMTIKEVTKL WIYQARYRIP
VSSAPPGSWV LIEGVDASIM KTATLCNASY DEDVYIFRAL KFNTLPVVKT ATEPLNPSEL
PKMVEGLRKI SKSYPLAITK VEESGEHTIL GTGELYLDSI IKDLRELYSE VQVKVADPVV
SFCETVVESS SMKCFAETPN KKNKLTMIAE PLDRGLAEDI ENGVVSIDWN RVQLGDFFRT
KYDWDLLAAR SIWAFGPDKQ GTNILLDDTL PTEVDRNLMM GVKDSIVQGF QWGAREGPLC
DEPIRNVKFK IVDARIAPEP LHRGSGQMIP TARRVAYSAF LMATPRLMEP VYYVEIQTPI
DCVTAIYTVL SRRRGYVTSD VPQPGTPAYI VKAFLPVIES FGFETDLRYH TQGQAFCLSV
FDHWAIVPGD PLDKAIQLRP LEPAPIQHLA REFMVKTRRR KGMSEDVSGN KFFDEAMMVE
LAQQTGDLHL QMI
