GFP_AEQVI
ID GFP_AEQVI Reviewed; 238 AA.
AC P42212; Q17104; Q27903; Q93125;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Green fluorescent protein;
GN Name=GFP;
OS Aequorea victoria (Water jellyfish) (Mesonema victoria).
OC Eukaryota; Metazoa; Cnidaria; Hydrozoa; Hydroidolina; Leptothecata;
OC Aequoreidae; Aequorea.
OX NCBI_TaxID=6100;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND VARIANTS TYR-100;
RP SER-108; MET-141 AND ILE-219.
RX PubMed=1347277; DOI=10.1016/0378-1119(92)90691-h;
RA Prasher D.C., Eckenrode V.K., Ward W.W., Prendergast F.G., Cormier M.J.;
RT "Primary structure of the Aequorea victoria green-fluorescent protein.";
RL Gene 111:229-233(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=8137953; DOI=10.1016/0014-5793(94)80472-9;
RA Inouye S., Tsuji F.I.;
RT "Aequorea green fluorescent protein. Expression of the gene and
RT fluorescence characteristics of the recombinant protein.";
RL FEBS Lett. 341:277-280(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, AND
RP BIOTECHNOLOGY.
RX PubMed=9154981; DOI=10.1023/a:1005740823703;
RA Rouwendal G.J.A., Mendes O., Wolbert E.J.H., de Boer A.D.;
RT "Enhanced expression in tobacco of the gene encoding green fluorescent
RT protein by modification of its codon usage.";
RL Plant Mol. Biol. 33:989-999(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOTECHNOLOGY, AND MUTAGENESIS OF
RP PHE-64; SER-65; VAL-68 AND SER-72.
RX PubMed=8707053; DOI=10.1016/0378-1119(95)00685-0;
RA Cormack B.P., Valdivia R.H., Falkow S.;
RT "FACS-optimized mutants of the green fluorescent protein (GFP).";
RL Gene 173:33-38(1996).
RN [5]
RP CHROMOPHORE, AND DEHYDROGENATION AT TYR-66.
RX PubMed=8448132; DOI=10.1021/bi00056a003;
RA Cody C.W., Prasher D.C., Westler W.M., Prendergast F.G., Ward W.W.;
RT "Chemical structure of the hexapeptide chromophore of the Aequorea green-
RT fluorescent protein.";
RL Biochemistry 32:1212-1218(1993).
RN [6]
RP MUTAGENESIS OF TYR-66; ILE-167; SER-202 AND THR-203.
RX PubMed=7809066; DOI=10.1073/pnas.91.26.12501;
RA Heim R., Prasher D.C., Tsien R.Y.;
RT "Wavelength mutations and posttranslational autoxidation of green
RT fluorescent protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:12501-12504(1994).
RN [7]
RP MUTAGENESIS OF PHE-64; SER-65 AND GLN-69.
RX PubMed=9634755; DOI=10.1038/nbt0295-151;
RA Delagrave S., Hawtin R.E., Silva C.M., Yang M.M., Youvan D.C.;
RT "Red-shifted excitation mutants of the green fluorescent protein.";
RL Biotechnology (N.Y.) 13:151-154(1995).
RN [8]
RP MUTAGENESIS OF THR-203 AND GLU-222.
RX PubMed=7796912; DOI=10.1016/0014-5793(95)00557-p;
RA Ehrig T., O'Kane D.J., Prendergast F.G.;
RT "Green-fluorescent protein mutants with altered fluorescence excitation
RT spectra.";
RL FEBS Lett. 367:163-166(1995).
RN [9]
RP MUTAGENESIS OF TYR-66, AND BIOTECHNOLOGY.
RX PubMed=8578587; DOI=10.1016/s0968-0004(00)89099-4;
RA Cubitt A.B., Heim R., Adams S.R., Boyd A.E., Gross L.A., Tsien R.Y.;
RT "Understanding, improving and using green fluorescent proteins.";
RL Trends Biochem. Sci. 20:448-455(1995).
RN [10]
RP MUTAGENESIS OF VAL-163 AND SER-175.
RX PubMed=8994830; DOI=10.1016/s0960-9822(02)70789-6;
RA Siemering K.R., Golbik R., Sever R., Haseloff J.;
RT "Mutations that suppress the thermosensitivity of green fluorescent
RT protein.";
RL Curr. Biol. 6:1653-1663(1996).
RN [11]
RP MUTAGENESIS OF PHE-99; MET-153 AND VAL-163.
RX PubMed=9630892; DOI=10.1038/nbt0396-315;
RA Crameri A., Whitehorn E.A., Tate E., Stemmer W.P.;
RT "Improved green fluorescent protein by molecular evolution using DNA
RT shuffling.";
RL Nat. Biotechnol. 14:315-319(1996).
RN [12]
RP MUTAGENESIS OF PHE-99; MET-153 AND VAL-163.
RX PubMed=9631088; DOI=10.1038/nbt1096-1252;
RA Yokoe H., Meyer T.;
RT "Spatial dynamics of GFP-tagged proteins investigated by local fluorescence
RT enhancement.";
RL Nat. Biotechnol. 14:1252-1256(1996).
RN [13]
RP MUTAGENESIS OF SER-147.
RX PubMed=9125154; DOI=10.1006/bbrc.1997.6235;
RA Kimata Y., Iwaki M., Lim C.R., Kohno K.;
RT "A novel mutation which enhances the fluorescence of green fluorescent
RT protein at high temperatures.";
RL Biochem. Biophys. Res. Commun. 232:69-73(1997).
RN [14]
RP MUTAGENESIS OF PHE-64; SER-65; TYR-66; PHE-99; MET-153 AND VAL-163.
RX PubMed=9370472; DOI=10.1016/s0006-3495(97)78307-3;
RA Patterson G.H., Knobel S.M., Sharif W.D., Kain S.R., Piston D.W.;
RT "Use of the green fluorescent protein and its mutants in quantitative
RT fluorescence microscopy.";
RL Biophys. J. 73:2782-2790(1997).
RN [15]
RP REVIEW ON MUTAGENESIS, AND BIOTECHNOLOGY.
RX PubMed=9759496; DOI=10.1146/annurev.biochem.67.1.509;
RA Tsien R.Y.;
RT "The green fluorescent protein.";
RL Annu. Rev. Biochem. 67:509-544(1998).
RN [16]
RP MUTAGENESIS OF SER-65; VAL-68; SER-72; ASN-146; MET-153; VAL-163 AND
RP THR-203.
RX PubMed=11299042; DOI=10.1186/1471-213x-1-4;
RA Srinivas S., Watanabe T., Lin C.S., William C.M., Tanabe Y., Jessell T.M.,
RA Costantini F.;
RT "Cre reporter strains produced by targeted insertion of EYFP and ECFP into
RT the ROSA26 locus.";
RL BMC Dev. Biol. 1:4-4(2001).
RN [17]
RP MUTAGENESIS OF PHE-64 AND SER-65.
RX PubMed=11776264;
RA Baumstark-Khan C., Hellweg C.E., Palm M., Horneck G.;
RT "Enhanced green fluorescent protein (EGFP) for space radiation research
RT using mammalian cells in the International Space Station.";
RL Phys. Med. 17:210-214(2001).
RN [18]
RP MUTAGENESIS OF SER-72; TYR-145 AND THR-203.
RX PubMed=12769828; DOI=10.1186/1472-6750-3-5;
RA Zapata-Hommer O., Griesbeck O.;
RT "Efficiently folding and circularly permuted variants of the Sapphire
RT mutant of GFP.";
RL BMC Biotechnol. 3:5-5(2003).
RN [19]
RP MUTAGENESIS OF PHE-64; SER-65; TYR-66; ASN-146; MET-153 AND VAL-163.
RX PubMed=15065907; DOI=10.1117/1.1646416;
RA Wang Z., Shah J.V., Chen Z., Sun C.H., Berns M.W.;
RT "Fluorescence correlation spectroscopy investigation of a GFP mutant-
RT enhanced cyan fluorescent protein and its tubulin fusion in living cells
RT with two-photon excitation.";
RL J. Biomed. Opt. 9:395-403(2004).
RN [20]
RP MUTAGENESIS OF PHE-64; SER-65; TYR-66; SER-72; TYR-145; ASN-146; HIS-148;
RP MET-153 AND VAL-163.
RX PubMed=14990965; DOI=10.1038/nbt945;
RA Rizzo M.A., Springer G.H., Granada B., Piston D.W.;
RT "An improved cyan fluorescent protein variant useful for FRET.";
RL Nat. Biotechnol. 22:445-449(2004).
RN [21]
RP MUTAGENESIS OF SER-30; PHE-46; GLN-69; ASP-103; TYR-145; VAL-176; VAL-193
RP AND ASN-198.
RX PubMed=16201762; DOI=10.1021/bi050550f;
RA Scruggs A.W., Flores C.L., Wachter R., Woodbury N.W.;
RT "Development and characterization of green fluorescent protein mutants with
RT altered lifetimes.";
RL Biochemistry 44:13377-13384(2005).
RN [22]
RP MUTAGENESIS OF ALA-206.
RX PubMed=16739159; DOI=10.1002/cphc.200600057;
RA Grailhe R., Merola F., Ridard J., Couvignou S., Le Poupon C.,
RA Changeux J.P., Laguitton-Pasquier H.;
RT "Monitoring protein interactions in the living cell through the
RT fluorescence decays of the cyan fluorescent protein.";
RL ChemPhysChem 7:1442-1454(2006).
RN [23]
RP MUTAGENESIS OF PHE-64; SER-65; SER-72; ASN-149; MET-153 AND ILE-167.
RX PubMed=17658219; DOI=10.1016/j.bbagen.2007.06.005;
RA Teerawanichpan P., Hoffman T., Ashe P., Datla R., Selvaraj G.;
RT "Investigations of combinations of mutations in the jellyfish green
RT fluorescent protein (GFP) that afford brighter fluorescence, and use of a
RT version (VisGreen) in plant, bacterial, and animal cells.";
RL Biochim. Biophys. Acta 1770:1360-1368(2007).
RN [24]
RP BIOTECHNOLOGY.
RX PubMed=17685514; DOI=10.1021/ja0715905;
RA Wong F.H., Banks D.S., Abu-Arish A., Fradin C.;
RT "A molecular thermometer based on fluorescent protein blinking.";
RL J. Am. Chem. Soc. 129:10302-10303(2007).
RN [25]
RP MUTAGENESIS OF PHE-46; PHE-64; VAL-68; LYS-162; VAL-163; ILE-167 AND
RP ILE-171.
RX PubMed=18366185; DOI=10.1021/bi702130s;
RA Mishin A.S., Subach F.V., Yampolsky I.V., King W., Lukyanov K.A.,
RA Verkhusha V.V.;
RT "The first mutant of the Aequorea victoria green fluorescent protein that
RT forms a red chromophore.";
RL Biochemistry 47:4666-4673(2008).
RN [26]
RP MUTAGENESIS OF SER-30; TYR-39; TYR-66; SER-72; GLN-80; ASN-105; ILE-128;
RP TYR-145; VAL-150; ASP-155; ILE-171; ASN-198; ALA-206 AND VAL-224.
RX PubMed=22891786; DOI=10.1021/jp306257t;
RA Park J.W., Rhee Y.M.;
RT "Interpolated mechanics-molecular mechanics study of internal rotation
RT dynamics of the chromophore unit in blue fluorescent protein and its
RT variants.";
RL J. Phys. Chem. B 116:11137-11147(2012).
RN [27]
RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
RX PubMed=9631087; DOI=10.1038/nbt1096-1246;
RA Yang F., Moss L.G., Phillips G.N. Jr.;
RT "The molecular structure of green fluorescent protein.";
RL Nat. Biotechnol. 14:1246-1251(1996).
RN [28] {ECO:0007744|PDB:1EMA}
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS), AND MUTAGENESIS OF SER-65 AND
RP THR-203.
RX PubMed=8703075; DOI=10.1126/science.273.5280.1392;
RA Ormo M., Cubitt A.B., Kallio K., Gross L.A., Tsien R.Y., Remington S.J.;
RT "Crystal structure of the Aequorea victoria green fluorescent protein.";
RL Science 273:1392-1395(1996).
RN [29] {ECO:0007744|PDB:1EMC, ECO:0007744|PDB:1EME, ECO:0007744|PDB:1EMF, ECO:0007744|PDB:1EMK, ECO:0007744|PDB:1EML, ECO:0007744|PDB:1EMM, ECO:0007744|PDB:2EMD, ECO:0007744|PDB:2EMN, ECO:0007744|PDB:2EMO}
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS), MUTAGENESIS OF PHE-64; TYR-66;
RP SER-72; ASN-149; MET-153 AND ILE-167, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP BIOTECHNOLOGY.
RX PubMed=9145105; DOI=10.1038/nsb0597-361;
RA Palm G.J., Zdanov A., Gaitanaris G.A., Stauber R., Pavlakis G.N.,
RA Wlodawer A.;
RT "The structural basis for spectral variations in green fluorescent
RT protein.";
RL Nat. Struct. Biol. 4:361-365(1997).
RN [30] {ECO:0007744|PDB:1YFP, ECO:0007744|PDB:2YFP}
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF YELLOW EMISSION MUTANT
RP TYR-203/GLY-65/LEU-68/ALA-72, MUTAGENESIS OF SER-65; VAL-68; SER-72 AND
RP THR-203, BIOPHYSICOCHEMICAL PROPERTIES, BIOTECHNOLOGY, AND SUBUNIT.
RX PubMed=9782051; DOI=10.1016/s0969-2126(98)00127-0;
RA Wachter R.M., Elsliger M.-A., Kallio K., Hanson G.T., Remington S.J.;
RT "Structural basis of spectral shifts in the yellow-emission variants of
RT green fluorescent protein.";
RL Structure 6:1267-1277(1998).
RN [31]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=10220315; DOI=10.1021/bi9902182;
RA Elsliger M.-A., Wachter R.M., Hanson G.T., Kallio K., Remington S.J.;
RT "Structural and spectral response of green fluorescent protein variants to
RT changes in pH.";
RL Biochemistry 38:5296-5301(1999).
RN [32] {ECO:0007744|PDB:1HUY}
RP X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 2-238 OF MUTANT CITRININE, AND
RP MUTAGENESIS OF VAL-68; GLN-69; SER-72 AND THR-203.
RX PubMed=11387331; DOI=10.1074/jbc.m102815200;
RA Griesbeck O., Baird G.S., Campbell R.E., Zacharias D.A., Tsien R.Y.;
RT "Reducing the environmental sensitivity of yellow fluorescent protein.
RT Mechanism and applications.";
RL J. Biol. Chem. 276:29188-29194(2001).
RN [33] {ECO:0007744|PDB:1MYW}
RP X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 2-238 OF VENUS, AND MUTAGENESIS
RP OF PHE-46; PHE-64; SER-65; VAL-68; SER-72; MET-153; VAL-163; SER-175 AND
RP THR-203.
RX PubMed=12370172; DOI=10.1074/jbc.m209524200;
RA Rekas A., Alattia J.R., Nagai T., Miyawaki A., Ikura M.;
RT "Crystal structure of venus, a yellow fluorescent protein with improved
RT maturation and reduced environmental sensitivity.";
RL J. Biol. Chem. 277:50573-50578(2002).
RN [34] {ECO:0007744|PDB:2Q57}
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS), MUTAGENESIS OF 65-SER-TYR-66,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND BIOTECHNOLOGY.
RX PubMed=17685554; DOI=10.1021/bi602664c;
RA Malo G.D., Pouwels L.J., Wang M., Weichsel A., Montfort W.R., Rizzo M.A.,
RA Piston D.W., Wachter R.M.;
RT "X-ray structure of Cerulean GFP: a tryptophan-based chromophore useful for
RT fluorescence lifetime imaging.";
RL Biochemistry 46:9865-9873(2007).
CC -!- FUNCTION: Energy-transfer acceptor. Its role is to transduce the blue
CC chemiluminescence of the protein aequorin into green fluorescent light
CC by energy transfer. Fluoresces in vivo upon receiving energy from the
CC Ca(2+)-activated photoprotein aequorin.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Absorption:
CC Abs(max)=395 nm;
CC Note=Exhibits a smaller absorbance peak at 470 nm. The fluorescence
CC emission spectrum peaks at 507-510 nm with a shoulder at 545 nm
CC (PubMed:8137953, PubMed:9154981). The exact value of the emission
CC maximum depends on the environment of the chromophore
CC (PubMed:10220315). As a consequence, mutant versions have been
CC designed that have substantially shifted emission spectra, including
CC yellow-emission variants (YFP), blue and cerulean fluorescing
CC proteins (PubMed:9145105, PubMed:9782051, PubMed:17685554).
CC {ECO:0000269|PubMed:10220315, ECO:0000269|PubMed:17685554,
CC ECO:0000269|PubMed:8137953, ECO:0000269|PubMed:9145105,
CC ECO:0000269|PubMed:9154981, ECO:0000269|PubMed:9782051};
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:9782051}.
CC -!- TISSUE SPECIFICITY: Photocytes.
CC -!- PTM: Contains a chromophore consisting of modified amino acid residues.
CC The chromophore is formed by autocatalytic backbone condensation
CC between Ser-65 and Gly-67, and oxidation of Tyr-66 to
CC didehydrotyrosine. Maturation of the chromophore requires nothing other
CC than molecular oxygen. {ECO:0000269|PubMed:8448132}.
CC -!- BIOTECHNOLOGY: Green fluorescent protein has been engineered to produce
CC a vast number of variously colored mutants, fusion proteins, and
CC biosensors. Green fluorescent protein can be mutated to emit at
CC different wavelengths such as blue for BFP (when Tyr-66 is replaced by
CC His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP
CC (when THR-203 is replaced by Tyr). Further generation of mutants led to
CC more stable proteins (at 37 degrees Celsius for example) with brighter
CC fluorescence and longer fluorescence lifetimes. Fluorescent proteins
CC and their mutated allelic forms have become a useful and ubiquitous
CC tool for making chimeric proteins, where they function as a fluorescent
CC protein tag. Typically they tolerate N- and C-terminal fusion to a
CC broad variety of proteins. They have been expressed in most known cell
CC types and are used as a noninvasive fluorescent marker in living cells
CC and organisms. They enable a wide range of applications where they have
CC functioned as a cell lineage tracer, reporter of gene expression, or as
CC a measure of protein-protein interactions (PubMed:17685514,
CC PubMed:17685554, PubMed:8578587, PubMed:8707053, PubMed:9145105,
CC PubMed:9154981, PubMed:9759496, PubMed:9782051). Can also be used as a
CC molecular thermometer, allowing accurate temperature measurements in
CC fluids. The measurement process relies on the detection of the blinking
CC of GFP using fluorescence correlation spectroscopy (PubMed:17685514).
CC {ECO:0000269|PubMed:17685514, ECO:0000269|PubMed:17685554,
CC ECO:0000269|PubMed:8578587, ECO:0000269|PubMed:8707053,
CC ECO:0000269|PubMed:9145105, ECO:0000269|PubMed:9154981,
CC ECO:0000269|PubMed:9759496, ECO:0000269|PubMed:9782051}.
CC -!- SIMILARITY: Belongs to the GFP family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=The greenest of us all
CC - Issue 11 of June 2001;
CC URL="https://web.expasy.org/spotlight/back_issues/011";
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Paint my thoughts - Issue
CC 108 of August 2009;
CC URL="https://web.expasy.org/spotlight/back_issues/108";
CC -!- WEB RESOURCE: Name=Wikipedia; Note=Green fluorescent protein entry;
CC URL="https://en.wikipedia.org/wiki/Green_fluorescent_protein";
CC ---------------------------------------------------------------------------
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DR EMBL; M62654; AAA27722.1; -; mRNA.
DR EMBL; M62653; AAA27721.1; -; mRNA.
DR EMBL; L29345; AAA58246.1; -; mRNA.
DR EMBL; X96418; CAA65278.1; -; mRNA.
DR EMBL; U73901; AAB18957.1; -; Genomic_DNA.
DR PIR; JS0692; JQ1514.
DR PDB; 1B9C; X-ray; 2.40 A; A/B/C/D=1-238.
DR PDB; 1BFP; X-ray; 2.10 A; A=1-238.
DR PDB; 1C4F; X-ray; 2.25 A; A=1-238.
DR PDB; 1CV7; X-ray; 2.50 A; A=1-228.
DR PDB; 1EMA; X-ray; 1.90 A; A=1-238.
DR PDB; 1EMB; X-ray; 2.13 A; A=1-238.
DR PDB; 1EMC; X-ray; 2.30 A; A/B/C/D=2-237.
DR PDB; 1EME; X-ray; 2.50 A; A=2-237.
DR PDB; 1EMF; X-ray; 2.40 A; A=2-238.
DR PDB; 1EMG; X-ray; 2.00 A; A=1-229.
DR PDB; 1EMK; X-ray; 2.10 A; A=2-237.
DR PDB; 1EML; X-ray; 2.30 A; A=2-237.
DR PDB; 1EMM; X-ray; 2.30 A; A=2-238.
DR PDB; 1F09; X-ray; 2.14 A; A=1-238.
DR PDB; 1F0B; X-ray; 2.10 A; A=1-238.
DR PDB; 1GFL; X-ray; 1.90 A; A/B=2-238.
DR PDB; 1H6R; X-ray; 1.50 A; A/B/C=1-238.
DR PDB; 1HCJ; X-ray; 1.80 A; A/B/C/D=1-238.
DR PDB; 1HUY; X-ray; 2.20 A; A=2-238.
DR PDB; 1JBY; X-ray; 1.80 A; A=1-238.
DR PDB; 1JBZ; X-ray; 1.50 A; A=1-238.
DR PDB; 1JC0; X-ray; 2.00 A; A/B/C=1-238.
DR PDB; 1JC1; X-ray; 1.90 A; A/B/C=1-238.
DR PDB; 1KP5; X-ray; 2.60 A; A/B=1-238.
DR PDB; 1KYP; X-ray; 1.35 A; A=2-238.
DR PDB; 1KYR; X-ray; 1.50 A; A=2-238.
DR PDB; 1KYS; X-ray; 1.44 A; A=2-238.
DR PDB; 1MYW; X-ray; 2.20 A; A=2-238.
DR PDB; 1Q4A; X-ray; 1.45 A; A=1-238.
DR PDB; 1Q4B; X-ray; 1.48 A; A=1-238.
DR PDB; 1Q4C; X-ray; 1.55 A; A=1-238.
DR PDB; 1Q4D; X-ray; 1.58 A; A=1-238.
DR PDB; 1Q4E; X-ray; 1.38 A; A=1-238.
DR PDB; 1Q73; X-ray; 1.60 A; A=1-238.
DR PDB; 1QXT; X-ray; 2.00 A; A=2-229.
DR PDB; 1QY3; X-ray; 2.00 A; A=1-229.
DR PDB; 1QYF; X-ray; 1.50 A; A=1-227.
DR PDB; 1QYO; X-ray; 1.80 A; A=1-238.
DR PDB; 1QYQ; X-ray; 1.80 A; A=2-238.
DR PDB; 1RM9; X-ray; 2.90 A; A=2-236.
DR PDB; 1RMM; X-ray; 1.90 A; A=2-227.
DR PDB; 1RMO; X-ray; 1.80 A; A=2-236.
DR PDB; 1RMP; X-ray; 3.00 A; A=2-229.
DR PDB; 1RRX; X-ray; 2.10 A; A=2-227.
DR PDB; 1S6Z; X-ray; 1.50 A; A=1-238.
DR PDB; 1W7S; X-ray; 1.85 A; A/B/C/D=1-238.
DR PDB; 1W7T; X-ray; 1.85 A; A/B/C/D=1-238.
DR PDB; 1W7U; X-ray; 1.85 A; A/B/C/D=1-238.
DR PDB; 1YFP; X-ray; 2.50 A; A/B=3-229.
DR PDB; 1YHG; X-ray; 2.50 A; A/B=2-238.
DR PDB; 1YHH; X-ray; 1.50 A; A=2-238.
DR PDB; 1YHI; X-ray; 1.90 A; A=2-238.
DR PDB; 1YJ2; X-ray; 1.50 A; A=2-238.
DR PDB; 1YJF; X-ray; 1.35 A; A=2-238.
DR PDB; 1Z1P; X-ray; 2.00 A; A=1-238.
DR PDB; 1Z1Q; X-ray; 1.50 A; A=1-238.
DR PDB; 2AH8; X-ray; 2.24 A; A/B=1-238.
DR PDB; 2AHA; X-ray; 1.98 A; A/B=1-238.
DR PDB; 2AWJ; X-ray; 1.60 A; A=2-229.
DR PDB; 2AWK; X-ray; 1.15 A; A=1-229.
DR PDB; 2AWL; X-ray; 1.85 A; A=1-229.
DR PDB; 2AWM; X-ray; 1.70 A; A=1-229.
DR PDB; 2B3P; X-ray; 1.40 A; A=1-238.
DR PDB; 2B3Q; X-ray; 2.30 A; A/B/C/D=1-238.
DR PDB; 2DUE; X-ray; 1.24 A; A=1-238.
DR PDB; 2DUF; X-ray; 1.50 A; A=1-238.
DR PDB; 2DUG; X-ray; 1.40 A; A=1-238.
DR PDB; 2DUH; X-ray; 1.20 A; A=1-238.
DR PDB; 2DUI; X-ray; 1.36 A; A=1-238.
DR PDB; 2EMD; X-ray; 2.00 A; A=1-238.
DR PDB; 2EMN; X-ray; 2.30 A; A=1-238.
DR PDB; 2EMO; X-ray; 2.60 A; A=1-238.
DR PDB; 2FWQ; X-ray; 1.40 A; A=2-238.
DR PDB; 2FZU; X-ray; 1.25 A; A=2-238.
DR PDB; 2G16; X-ray; 2.00 A; A=2-64, B=67-238.
DR PDB; 2G2S; X-ray; 1.20 A; A=2-63, B=66-238.
DR PDB; 2G3D; X-ray; 1.35 A; A=2-63, B=66-238.
DR PDB; 2G5Z; X-ray; 1.80 A; A=2-65, B=66-238.
DR PDB; 2G6E; X-ray; 1.30 A; A=2-238.
DR PDB; 2H6V; X-ray; 1.47 A; A=2-238.
DR PDB; 2H9W; X-ray; 1.82 A; A=2-237.
DR PDB; 2HCG; X-ray; 1.35 A; A=2-238.
DR PDB; 2HFC; X-ray; 1.20 A; A=2-238.
DR PDB; 2HGD; X-ray; 1.60 A; A=2-238.
DR PDB; 2HGY; X-ray; 2.05 A; A=2-238.
DR PDB; 2HJO; X-ray; 1.25 A; A=1-238.
DR PDB; 2HQZ; X-ray; 1.20 A; A=1-238.
DR PDB; 2HRS; X-ray; 1.40 A; A=1-238.
DR PDB; 2JAD; X-ray; 2.70 A; A=1-238.
DR PDB; 2O24; X-ray; 1.45 A; A=2-238.
DR PDB; 2O29; X-ray; 1.80 A; A=2-238.
DR PDB; 2O2B; X-ray; 1.94 A; A=2-238.
DR PDB; 2OKW; X-ray; 1.90 A; A/B/C/D/E/F=1-238.
DR PDB; 2OKY; X-ray; 2.40 A; A/B=1-238.
DR PDB; 2Q57; X-ray; 2.00 A; A=1-238.
DR PDB; 2Q6P; X-ray; 2.10 A; A=1-238.
DR PDB; 2QRF; X-ray; 1.50 A; A=1-230.
DR PDB; 2QT2; X-ray; 1.31 A; A=1-238.
DR PDB; 2QU1; X-ray; 1.70 A; A=1-238.
DR PDB; 2QZ0; X-ray; 1.20 A; A=2-229.
DR PDB; 2WSN; X-ray; 1.37 A; A=2-238.
DR PDB; 2WSO; X-ray; 1.15 A; A=2-238.
DR PDB; 2WUR; X-ray; 0.90 A; A=1-237.
DR PDB; 2Y0G; X-ray; 1.50 A; A=2-238.
DR PDB; 2YDZ; X-ray; 1.59 A; A=2-238.
DR PDB; 2YE0; X-ray; 1.47 A; A=2-238.
DR PDB; 2YE1; X-ray; 1.63 A; A=2-238.
DR PDB; 2YFP; X-ray; 2.60 A; A=1-238.
DR PDB; 3AI4; X-ray; 1.60 A; A=1-230.
DR PDB; 3AI5; X-ray; 1.40 A; A=1-230.
DR PDB; 3CB9; X-ray; 1.31 A; A=2-238.
DR PDB; 3CBE; X-ray; 1.49 A; A=2-238.
DR PDB; 3CD1; X-ray; 1.31 A; A=2-238.
DR PDB; 3CD9; X-ray; 1.50 A; A=2-238.
DR PDB; 3DPW; X-ray; 1.59 A; A=2-238.
DR PDB; 3DPX; X-ray; 1.50 A; A=2-238.
DR PDB; 3DPZ; X-ray; 1.70 A; A=2-238.
DR PDB; 3DQ1; X-ray; 1.70 A; A=2-238.
DR PDB; 3DQ2; X-ray; 1.60 A; A=2-238.
DR PDB; 3DQ3; X-ray; 1.70 A; A=2-238.
DR PDB; 3DQ4; X-ray; 1.47 A; A=2-238.
DR PDB; 3DQ5; X-ray; 1.50 A; A=2-238.
DR PDB; 3DQ6; X-ray; 1.60 A; A=2-238.
DR PDB; 3DQ7; X-ray; 1.23 A; A=2-238.
DR PDB; 3DQ8; X-ray; 1.51 A; A=2-238.
DR PDB; 3DQ9; X-ray; 1.40 A; A=2-238.
DR PDB; 3DQA; X-ray; 1.44 A; A=2-238.
DR PDB; 3DQC; X-ray; 1.49 A; A=2-238.
DR PDB; 3DQD; X-ray; 1.40 A; A=2-238.
DR PDB; 3DQE; X-ray; 1.43 A; A=2-238.
DR PDB; 3DQF; X-ray; 1.46 A; A=2-238.
DR PDB; 3DQH; X-ray; 1.45 A; A=2-238.
DR PDB; 3DQI; X-ray; 1.42 A; A=2-238.
DR PDB; 3DQJ; X-ray; 1.51 A; A=2-238.
DR PDB; 3DQK; X-ray; 1.40 A; A=2-238.
DR PDB; 3DQL; X-ray; 1.47 A; A=2-238.
DR PDB; 3DQM; X-ray; 1.44 A; A=2-238.
DR PDB; 3DQN; X-ray; 1.44 A; A=2-238.
DR PDB; 3DQO; X-ray; 1.50 A; A=2-238.
DR PDB; 3DQU; X-ray; 1.42 A; A=2-238.
DR PDB; 3ED8; X-ray; 2.70 A; A/B/C/D/E=2-238.
DR PDB; 3EK4; X-ray; 2.65 A; A=2-238.
DR PDB; 3EK7; X-ray; 1.85 A; A=2-238.
DR PDB; 3EK8; X-ray; 2.80 A; A=2-238.
DR PDB; 3EKH; X-ray; 2.00 A; A=2-238.
DR PDB; 3EKJ; X-ray; 2.80 A; A=2-238.
DR PDB; 3EVP; X-ray; 1.45 A; A=2-144.
DR PDB; 3EVR; X-ray; 2.00 A; A=2-144.
DR PDB; 3EVU; X-ray; 1.75 A; A=2-144.
DR PDB; 3EVV; X-ray; 2.60 A; A=2-238.
DR PDB; 3G9A; X-ray; 1.61 A; A=1-238.
DR PDB; 3GEX; X-ray; 1.60 A; A=1-238.
DR PDB; 3GJ1; X-ray; 1.80 A; A/B/C/D=1-230.
DR PDB; 3GJ2; X-ray; 1.90 A; A/B/C/D=1-230.
DR PDB; 3I19; X-ray; 1.36 A; A=1-238.
DR PDB; 3K1K; X-ray; 2.15 A; A/B=1-238.
DR PDB; 3LA1; X-ray; 1.29 A; A=1-238.
DR PDB; 3O77; X-ray; 2.35 A; A=2-238.
DR PDB; 3O78; X-ray; 2.60 A; A/B=2-237.
DR PDB; 3OGO; X-ray; 2.80 A; A/B/C/D=1-238.
DR PDB; 3OSQ; X-ray; 1.90 A; A=2-238.
DR PDB; 3OSR; X-ray; 2.00 A; A/B=2-238.
DR PDB; 3P28; X-ray; 1.80 A; A=3-229.
DR PDB; 3SG2; X-ray; 2.00 A; A=2-238.
DR PDB; 3SG3; X-ray; 2.10 A; A=2-238.
DR PDB; 3SG4; X-ray; 2.40 A; A=2-238.
DR PDB; 3SG5; X-ray; 1.90 A; A=2-238.
DR PDB; 3SG6; X-ray; 1.70 A; A=2-238.
DR PDB; 3SG7; X-ray; 1.90 A; A=2-238.
DR PDB; 3SRY; X-ray; 1.16 A; A=2-238.
DR PDB; 3SS0; X-ray; 1.49 A; A=2-238.
DR PDB; 3SSH; X-ray; 1.28 A; A=2-238.
DR PDB; 3SSK; X-ray; 1.36 A; A=2-238.
DR PDB; 3SSL; X-ray; 1.45 A; A=2-238.
DR PDB; 3SSP; X-ray; 1.63 A; A=2-238.
DR PDB; 3SST; X-ray; 1.40 A; A=2-238.
DR PDB; 3SSV; X-ray; 1.86 A; A=2-238.
DR PDB; 3SSY; X-ray; 1.77 A; A=2-238.
DR PDB; 3ST0; X-ray; 1.19 A; A=2-238.
DR PDB; 3SV5; X-ray; 1.53 A; A=2-238.
DR PDB; 3SVB; X-ray; 1.30 A; A=2-238.
DR PDB; 3SVC; X-ray; 1.31 A; A=2-238.
DR PDB; 3SVD; X-ray; 1.78 A; A=2-238.
DR PDB; 3SVE; X-ray; 1.49 A; A=2-238.
DR PDB; 3U8P; X-ray; 2.75 A; A/B/C=2-238.
DR PDB; 3UFZ; X-ray; 1.85 A; A=2-229.
DR PDB; 3UG0; X-ray; 2.09 A; A=2-229.
DR PDB; 3V3D; X-ray; 1.95 A; A=2-238.
DR PDB; 3VHT; X-ray; 2.40 A; A=1-230.
DR PDB; 3W1C; X-ray; 1.30 A; A=2-238.
DR PDB; 3W1D; X-ray; 1.50 A; A=2-238.
DR PDB; 3WLC; X-ray; 2.49 A; A=2-238.
DR PDB; 3WLD; X-ray; 2.70 A; A=2-144.
DR PDB; 3ZTF; X-ray; 1.31 A; A=2-238.
DR PDB; 4ANJ; X-ray; 2.60 A; A=1-238.
DR PDB; 4AR7; X-ray; 1.23 A; A=2-238.
DR PDB; 4AS8; X-ray; 1.02 A; A=2-238.
DR PDB; 4B5Y; X-ray; 1.45 A; A=2-238.
DR PDB; 4BDU; X-ray; 3.00 A; A/B/C/D=1-230.
DR PDB; 4EN1; X-ray; 1.62 A; A/B=2-238.
DR PDB; 4EUL; X-ray; 1.35 A; A=2-238.
DR PDB; 4GES; X-ray; 1.23 A; B=1-238.
DR PDB; 4GF6; X-ray; 1.10 A; B=1-237.
DR PDB; 4H47; X-ray; 1.90 A; A=1-238.
DR PDB; 4H48; X-ray; 1.45 A; A=1-238.
DR PDB; 4IK1; X-ray; 2.00 A; A=2-144.
DR PDB; 4IK3; X-ray; 2.01 A; A=149-238, A=2-142.
DR PDB; 4IK4; X-ray; 2.01 A; A=2-142, A=149-238.
DR PDB; 4IK5; X-ray; 2.50 A; A=2-142, A=149-238.
DR PDB; 4IK8; X-ray; 1.55 A; A=2-144, A=149-238.
DR PDB; 4IK9; X-ray; 1.80 A; A=2-144.
DR PDB; 4J88; X-ray; 2.08 A; A/B=2-238.
DR PDB; 4J89; X-ray; 2.10 A; A/B=2-238.
DR PDB; 4J8A; X-ray; 1.26 A; A=2-238.
DR PDB; 4JFG; X-ray; 3.00 A; A/B/C/D/E/F/G/H=1-238.
DR PDB; 4JRB; X-ray; 2.41 A; A=1-229.
DR PDB; 4KA9; X-ray; 1.58 A; A=5-238.
DR PDB; 4KAG; X-ray; 1.12 A; A=1-238.
DR PDB; 4KEX; X-ray; 1.60 A; A=1-238.
DR PDB; 4KF5; X-ray; 2.60 A; A/B=1-196.
DR PDB; 4KW4; X-ray; 1.75 A; A=2-238.
DR PDB; 4KW8; X-ray; 2.46 A; A=2-238.
DR PDB; 4KW9; X-ray; 1.80 A; A=2-238.
DR PDB; 4L12; X-ray; 1.78 A; A=2-230.
DR PDB; 4L13; X-ray; 1.66 A; A=2-230.
DR PDB; 4L1I; X-ray; 1.20 A; A=2-230.
DR PDB; 4LQT; X-ray; 1.10 A; A=2-238.
DR PDB; 4LQU; X-ray; 1.60 A; A/B/C/D=2-238.
DR PDB; 4LW5; X-ray; 2.55 A; A/B/C/D/E=2-238.
DR PDB; 4N3D; X-ray; 1.34 A; A/B=1-230.
DR PDB; 4NDJ; X-ray; 1.85 A; A=2-238.
DR PDB; 4NDK; X-ray; 2.30 A; A/B=2-238.
DR PDB; 4OGS; X-ray; 2.21 A; A/B=1-238.
DR PDB; 4ORN; X-ray; 1.71 A; A/B=2-238.
DR PDB; 4P1Q; X-ray; 1.50 A; A=3-231.
DR PDB; 4P7H; X-ray; 3.20 A; A/B=5-238.
DR PDB; 4PA0; X-ray; 2.25 A; A/B=5-234.
DR PDB; 4PFE; X-ray; 2.60 A; A/B=2-229.
DR PDB; 4U2V; X-ray; 2.30 A; A/B/C/D=1-230.
DR PDB; 4XBI; X-ray; 2.01 A; A/B=2-230.
DR PDB; 4XGY; X-ray; 1.49 A; A=2-238.
DR PDB; 4XL5; X-ray; 2.00 A; A=2-238.
DR PDB; 4XOV; X-ray; 1.20 A; A=2-238.
DR PDB; 4XOW; X-ray; 1.25 A; A=2-238.
DR PDB; 4XVP; X-ray; 3.40 A; A/B/C=2-238.
DR PDB; 4Z4K; X-ray; 2.80 A; A/B=1-230.
DR PDB; 4Z4M; X-ray; 2.15 A; A/B=1-230.
DR PDB; 4ZF3; X-ray; 1.90 A; A/B=51-236.
DR PDB; 4ZF4; X-ray; 1.82 A; A/B=4-237.
DR PDB; 4ZF5; X-ray; 1.70 A; A/B=4-237.
DR PDB; 5AQB; X-ray; 1.37 A; B=2-231.
DR PDB; 5B61; X-ray; 3.12 A; A/B/C/D/E/F=1-238.
DR PDB; 5BKF; EM; 3.60 A; E=2-238.
DR PDB; 5BKG; EM; 3.80 A; E=2-238.
DR PDB; 5DPG; X-ray; 1.85 A; A=2-238.
DR PDB; 5DPH; X-ray; 1.42 A; A/B=2-238.
DR PDB; 5DPI; X-ray; 2.54 A; A/B/C/D/E/F=2-238.
DR PDB; 5DPJ; X-ray; 2.50 A; A/B/C/D=2-238.
DR PDB; 5DQB; X-ray; 1.25 A; A=2-238.
DR PDB; 5DQM; X-ray; 1.30 A; A=2-238.
DR PDB; 5DRF; X-ray; 1.14 A; A=2-238.
DR PDB; 5DRG; X-ray; 1.14 A; A=2-238.
DR PDB; 5DTX; X-ray; 1.45 A; A=1-63, A=65-238.
DR PDB; 5DTY; X-ray; 1.50 A; A=1-63, A=65-238.
DR PDB; 5DTZ; X-ray; 1.50 A; A/B/C/D=2-238.
DR PDB; 5DU0; X-ray; 2.35 A; A/B/C/D=2-238.
DR PDB; 5EHU; X-ray; 1.45 A; A/B=2-238.
DR PDB; 5F9G; X-ray; 2.77 A; A=2-144.
DR PDB; 5FGU; X-ray; 1.90 A; A=1-229.
DR PDB; 5HBD; X-ray; 1.65 A; A=1-238.
DR PDB; 5HGE; X-ray; 1.86 A; A=1-238.
DR PDB; 5HW9; X-ray; 3.00 A; A=1-238.
DR PDB; 5HZO; X-ray; 2.49 A; A/B=1-235.
DR PDB; 5J2O; X-ray; 1.50 A; A=2-238.
DR PDB; 5J3N; X-ray; 2.45 A; A/B=2-238.
DR PDB; 5JZK; X-ray; 1.90 A; A/B=3-238.
DR PDB; 5JZL; X-ray; 1.80 A; A/B=3-238.
DR PDB; 5KTG; X-ray; 2.80 A; A/B=1-230.
DR PDB; 5LEL; X-ray; 3.10 A; C/F/I=2-238.
DR PDB; 5LEM; X-ray; 2.98 A; C=2-238.
DR PDB; 5MA3; X-ray; 1.70 A; B=2-238.
DR PDB; 5MA4; X-ray; 1.40 A; B=2-238.
DR PDB; 5MA5; X-ray; 1.85 A; B/D=2-238.
DR PDB; 5MA6; X-ray; 2.30 A; A=2-238.
DR PDB; 5MA8; X-ray; 2.35 A; B/D=2-238.
DR PDB; 5MA9; X-ray; 1.57 A; B/D/F/H=2-238.
DR PDB; 5MAD; X-ray; 1.53 A; B/D/F/H=2-238.
DR PDB; 5MAK; X-ray; 2.50 A; B/D=2-238.
DR PDB; 5MSE; X-ray; 1.66 A; A/B/C/D=2-238.
DR PDB; 5N9O; X-ray; 1.53 A; A=2-238.
DR PDB; 5NHN; X-ray; 1.96 A; A/B=3-231.
DR PDB; 5NI3; X-ray; 1.28 A; A=3-229, B/C/D=3-233.
DR PDB; 5O89; X-ray; 1.70 A; A=2-238.
DR PDB; 5O8B; X-ray; 1.70 A; A=2-238.
DR PDB; 5O8C; X-ray; 1.70 A; A=2-238.
DR PDB; 5OX8; X-ray; 1.29 A; A=2-238.
DR PDB; 5OX9; X-ray; 1.56 A; A=2-238.
DR PDB; 5OXA; X-ray; 1.16 A; A=2-238.
DR PDB; 5OXB; X-ray; 1.38 A; A=2-238.
DR PDB; 5OXC; X-ray; 1.02 A; A=1-238.
DR PDB; 5T3I; X-ray; 1.60 A; A=2-238.
DR PDB; 5WJ2; X-ray; 2.41 A; A/B=2-238.
DR PDB; 5WJ3; X-ray; 1.35 A; A/B=3-238.
DR PDB; 5WJ4; X-ray; 1.63 A; A/B=3-238.
DR PDB; 5Z6Y; X-ray; 1.87 A; A=3-230.
DR PDB; 6AA2; X-ray; 2.30 A; A/B/C=2-238.
DR PDB; 6AA6; X-ray; 2.39 A; A/B=2-238.
DR PDB; 6AS9; X-ray; 1.75 A; A=1-238.
DR PDB; 6B7R; X-ray; 1.73 A; A/B=2-214.
DR PDB; 6B7T; X-ray; 1.91 A; A/B=3-194, A/B=214-237.
DR PDB; 6B9C; X-ray; 1.70 A; A=3-238.
DR PDB; 6DQ0; X-ray; 2.05 A; A/B=1-238.
DR PDB; 6DQ1; X-ray; 1.60 A; A=1-238.
DR PDB; 6EFR; X-ray; 2.40 A; A=1-145.
DR PDB; 6FLL; X-ray; 1.79 A; A=2-238.
DR PDB; 6FWW; X-ray; 1.13 A; A=2-238.
DR PDB; 6GEL; X-ray; 2.51 A; A/B=2-227.
DR PDB; 6GEZ; X-ray; 2.47 A; A/B=2-227.
DR PDB; 6GO8; X-ray; 1.65 A; A=1-238.
DR PDB; 6GO9; X-ray; 1.67 A; A=1-238.
DR PDB; 6GQG; X-ray; 1.79 A; A=1-238.
DR PDB; 6GQH; X-ray; 2.40 A; A=1-238.
DR PDB; 6GRM; X-ray; 2.30 A; A=1-238.
DR PDB; 6HR1; X-ray; 1.90 A; A/B=2-238.
DR PDB; 6HUT; X-ray; 1.29 A; A=1-238.
DR PDB; 6IR6; X-ray; 1.64 A; A=2-238.
DR PDB; 6IR7; X-ray; 1.28 A; A=2-237.
DR PDB; 6ITC; EM; 3.45 A; G=1-238.
DR PDB; 6JGH; X-ray; 0.94 A; A=2-231.
DR PDB; 6JGI; X-ray; 0.85 A; A=2-231.
DR PDB; 6JGJ; X-ray; 0.78 A; A=2-231.
DR PDB; 6KKZ; X-ray; 0.90 A; A=2-231.
DR PDB; 6KL0; X-ray; 0.80 A; A=2-231.
DR PDB; 6KL1; X-ray; 0.85 A; A=2-232.
DR PDB; 6KRG; X-ray; 1.40 A; A=1-236.
DR PDB; 6L27; X-ray; 0.77 A; A=4-231.
DR PDB; 6LNP; X-ray; 2.99 A; B/D=1-111, B/D=150-238.
DR PDB; 6LR7; X-ray; 1.67 A; A=1-238.
DR PDB; 6MB2; EM; 5.00 A; a/b/c/d/e/f/g/h/i/j/k/l/m/n/o=2-229.
DR PDB; 6MDR; EM; 3.47 A; b/d/f/h/j/l/n/p=1-232.
DR PDB; 6MKS; EM; 3.40 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d=1-230.
DR PDB; 6MWQ; EM; 3.00 A; G/H/I/J=2-230.
DR PDB; 6OA8; X-ray; 1.37 A; A=3-238.
DR PDB; 6OFK; X-ray; 1.15 A; A/B=49-237.
DR PDB; 6OFL; X-ray; 1.25 A; A/B=49-237.
DR PDB; 6OFM; X-ray; 1.48 A; A/B=49-237.
DR PDB; 6OFN; X-ray; 1.65 A; A/B=49-237.
DR PDB; 6OFO; X-ray; 2.60 A; A/B=2-194.
DR PDB; 6OG8; X-ray; 1.60 A; A/B=49-237.
DR PDB; 6OG9; X-ray; 1.80 A; A/B=49-237.
DR PDB; 6OGA; X-ray; 1.60 A; A/B=49-237.
DR PDB; 6OGB; X-ray; 1.65 A; A/B=49-237.
DR PDB; 6OGC; X-ray; 1.18 A; A/B=49-237.
DR PDB; 6PFR; X-ray; 1.51 A; A=1-238.
DR PDB; 6PFS; X-ray; 1.76 A; A=1-238.
DR PDB; 6PFT; X-ray; 1.45 A; A=1-238.
DR PDB; 6PFU; X-ray; 1.62 A; A=1-238.
DR PDB; 6QQ8; X-ray; 1.46 A; A=2-238.
DR PDB; 6QQ9; X-ray; 1.82 A; A=2-238.
DR PDB; 6QQA; X-ray; 1.66 A; A=2-238.
DR PDB; 6QQB; X-ray; 2.45 A; A=2-238.
DR PDB; 6QUH; X-ray; 1.50 A; A/C=4-230.
DR PDB; 6QUI; X-ray; 1.94 A; A/D=2-230.
DR PDB; 6QUJ; X-ray; 1.68 A; A/D=1-230.
DR PDB; 6SM0; X-ray; 1.91 A; A=2-230.
DR PDB; 6T39; X-ray; 1.60 A; A=2-238.
DR PDB; 6T3A; X-ray; 1.85 A; A=2-238.
DR PDB; 6T90; EM; 3.05 A; K=2-238.
DR PDB; 6UHJ; X-ray; 1.50 A; A=1-238.
DR PDB; 6UHK; X-ray; 1.90 A; A/B=1-238.
DR PDB; 6UHL; X-ray; 1.91 A; A/B=1-238.
DR PDB; 6UHM; X-ray; 2.10 A; A/B=1-238.
DR PDB; 6UHN; X-ray; 1.92 A; A/B=1-238.
DR PDB; 6UHO; X-ray; 1.95 A; A/B=1-238.
DR PDB; 6UHP; X-ray; 2.90 A; A/B=1-238.
DR PDB; 6UHQ; X-ray; 2.85 A; A=1-238.
DR PDB; 6UHR; X-ray; 3.00 A; A/B=1-238.
DR PDB; 6UN4; X-ray; 1.50 A; A=2-238.
DR PDB; 6UN5; X-ray; 1.36 A; A/B=51-63, A/B=145-237.
DR PDB; 6UN6; X-ray; 1.50 A; A/B=51-63, A/B=145-237.
DR PDB; 6UN7; X-ray; 1.50 A; A/B=51-63, A/B=145-237.
DR PDB; 6UZ0; EM; 3.24 A; A=2-238.
DR PDB; 6UZ3; EM; 3.50 A; A=2-238.
DR PDB; 6VAL; EM; 3.87 A; A/B/C/D/E/F/G/H/I/J/K=2-238.
DR PDB; 6VAM; EM; 3.63 A; A/B/C/D/E/F/G/H=2-238.
DR PDB; 6VIO; X-ray; 3.60 A; A/B/C/D/E/F/G/H=2-238.
DR PDB; 6WRF; EM; 3.14 A; S=3-229.
DR PDB; 6WSG; EM; 3.16 A; S=3-232.
DR PDB; 6WV3; X-ray; 2.20 A; A=1-63, A=146-231.
DR PDB; 6WV4; X-ray; 3.01 A; A=1-63, A=146-231.
DR PDB; 6WV5; X-ray; 2.80 A; A=1-63, A=146-231.
DR PDB; 6WV6; X-ray; 2.70 A; A=1-63, A=146-231.
DR PDB; 6WV7; X-ray; 2.48 A; A/B=1-63, A/B=146-231.
DR PDB; 6WV8; X-ray; 3.01 A; A=1-63, A=146-238.
DR PDB; 6WV9; X-ray; 3.35 A; A=1-238.
DR PDB; 6WVA; X-ray; 3.35 A; A=1-238.
DR PDB; 6WVB; X-ray; 2.87 A; A=1-63, A=146-238.
DR PDB; 6WVD; X-ray; 2.25 A; A=1-231.
DR PDB; 6WVE; X-ray; 2.43 A; A=1-231.
DR PDB; 6WVF; X-ray; 2.90 A; A=1-231.
DR PDB; 6WVG; X-ray; 2.90 A; A/B=1-230.
DR PDB; 6WVH; X-ray; 1.99 A; A/B=1-63, A/B=146-231.
DR PDB; 6WVI; X-ray; 2.40 A; A=1-238.
DR PDB; 6XZF; X-ray; 1.80 A; A=1-238.
DR PDB; 6YOV; EM; 3.42 A; K=2-238.
DR PDB; 6ZUI; X-ray; 2.20 A; A=2-144.
DR PDB; 7A7K; X-ray; 1.55 A; A=2-238.
DR PDB; 7A7L; X-ray; 1.30 A; A=3-238.
DR PDB; 7AA5; EM; 4.18 A; A/B/C/D=3-238.
DR PDB; 7BWN; X-ray; 2.40 A; A/C/E/F/H/J/M/O=1-238.
DR PDB; 7BYL; EM; 2.50 A; A/C/E/G=2-238.
DR PDB; 7BYM; EM; 3.10 A; A/C/E/G=2-238.
DR PDB; 7BYN; EM; 3.30 A; A/C/E/G=2-238.
DR PDB; 7CD7; X-ray; 1.70 A; B/D=3-238.
DR PDB; 7CD8; X-ray; 2.00 A; B=3-238.
DR PDB; 7EDJ; EM; 3.30 A; I/J/K=2-238.
DR PDB; 7K18; EM; 3.30 A; A=2-238.
DR PDB; 7KM4; X-ray; 2.65 A; A=1-238.
DR PDB; 7KS0; EM; 5.30 A; A/C=2-238.
DR PDB; 7KS3; EM; 5.80 A; A/C=2-238.
DR PDB; 7KUY; EM; 3.60 A; E=2-238.
DR PDB; 7L31; EM; 3.80 A; E=2-238.
DR PDB; 7PCA; X-ray; 1.05 A; A=1-238.
DR PDB; 7PCZ; X-ray; 1.35 A; A/B=1-238.
DR PDB; 7PD0; X-ray; 2.00 A; A/B/C/D=1-238.
DR PDB; 7PNN; X-ray; 1.43 A; A=2-238.
DR PDBsum; 1B9C; -.
DR PDBsum; 1BFP; -.
DR PDBsum; 1C4F; -.
DR PDBsum; 1CV7; -.
DR PDBsum; 1EMA; -.
DR PDBsum; 1EMB; -.
DR PDBsum; 1EMC; -.
DR PDBsum; 1EME; -.
DR PDBsum; 1EMF; -.
DR PDBsum; 1EMG; -.
DR PDBsum; 1EMK; -.
DR PDBsum; 1EML; -.
DR PDBsum; 1EMM; -.
DR PDBsum; 1F09; -.
DR PDBsum; 1F0B; -.
DR PDBsum; 1GFL; -.
DR PDBsum; 1H6R; -.
DR PDBsum; 1HCJ; -.
DR PDBsum; 1HUY; -.
DR PDBsum; 1JBY; -.
DR PDBsum; 1JBZ; -.
DR PDBsum; 1JC0; -.
DR PDBsum; 1JC1; -.
DR PDBsum; 1KP5; -.
DR PDBsum; 1KYP; -.
DR PDBsum; 1KYR; -.
DR PDBsum; 1KYS; -.
DR PDBsum; 1MYW; -.
DR PDBsum; 1Q4A; -.
DR PDBsum; 1Q4B; -.
DR PDBsum; 1Q4C; -.
DR PDBsum; 1Q4D; -.
DR PDBsum; 1Q4E; -.
DR PDBsum; 1Q73; -.
DR PDBsum; 1QXT; -.
DR PDBsum; 1QY3; -.
DR PDBsum; 1QYF; -.
DR PDBsum; 1QYO; -.
DR PDBsum; 1QYQ; -.
DR PDBsum; 1RM9; -.
DR PDBsum; 1RMM; -.
DR PDBsum; 1RMO; -.
DR PDBsum; 1RMP; -.
DR PDBsum; 1RRX; -.
DR PDBsum; 1S6Z; -.
DR PDBsum; 1W7S; -.
DR PDBsum; 1W7T; -.
DR PDBsum; 1W7U; -.
DR PDBsum; 1YFP; -.
DR PDBsum; 1YHG; -.
DR PDBsum; 1YHH; -.
DR PDBsum; 1YHI; -.
DR PDBsum; 1YJ2; -.
DR PDBsum; 1YJF; -.
DR PDBsum; 1Z1P; -.
DR PDBsum; 1Z1Q; -.
DR PDBsum; 2AH8; -.
DR PDBsum; 2AHA; -.
DR PDBsum; 2AWJ; -.
DR PDBsum; 2AWK; -.
DR PDBsum; 2AWL; -.
DR PDBsum; 2AWM; -.
DR PDBsum; 2B3P; -.
DR PDBsum; 2B3Q; -.
DR PDBsum; 2DUE; -.
DR PDBsum; 2DUF; -.
DR PDBsum; 2DUG; -.
DR PDBsum; 2DUH; -.
DR PDBsum; 2DUI; -.
DR PDBsum; 2EMD; -.
DR PDBsum; 2EMN; -.
DR PDBsum; 2EMO; -.
DR PDBsum; 2FWQ; -.
DR PDBsum; 2FZU; -.
DR PDBsum; 2G16; -.
DR PDBsum; 2G2S; -.
DR PDBsum; 2G3D; -.
DR PDBsum; 2G5Z; -.
DR PDBsum; 2G6E; -.
DR PDBsum; 2H6V; -.
DR PDBsum; 2H9W; -.
DR PDBsum; 2HCG; -.
DR PDBsum; 2HFC; -.
DR PDBsum; 2HGD; -.
DR PDBsum; 2HGY; -.
DR PDBsum; 2HJO; -.
DR PDBsum; 2HQZ; -.
DR PDBsum; 2HRS; -.
DR PDBsum; 2JAD; -.
DR PDBsum; 2O24; -.
DR PDBsum; 2O29; -.
DR PDBsum; 2O2B; -.
DR PDBsum; 2OKW; -.
DR PDBsum; 2OKY; -.
DR PDBsum; 2Q57; -.
DR PDBsum; 2Q6P; -.
DR PDBsum; 2QRF; -.
DR PDBsum; 2QT2; -.
DR PDBsum; 2QU1; -.
DR PDBsum; 2QZ0; -.
DR PDBsum; 2WSN; -.
DR PDBsum; 2WSO; -.
DR PDBsum; 2WUR; -.
DR PDBsum; 2Y0G; -.
DR PDBsum; 2YDZ; -.
DR PDBsum; 2YE0; -.
DR PDBsum; 2YE1; -.
DR PDBsum; 2YFP; -.
DR PDBsum; 3AI4; -.
DR PDBsum; 3AI5; -.
DR PDBsum; 3CB9; -.
DR PDBsum; 3CBE; -.
DR PDBsum; 3CD1; -.
DR PDBsum; 3CD9; -.
DR PDBsum; 3DPW; -.
DR PDBsum; 3DPX; -.
DR PDBsum; 3DPZ; -.
DR PDBsum; 3DQ1; -.
DR PDBsum; 3DQ2; -.
DR PDBsum; 3DQ3; -.
DR PDBsum; 3DQ4; -.
DR PDBsum; 3DQ5; -.
DR PDBsum; 3DQ6; -.
DR PDBsum; 3DQ7; -.
DR PDBsum; 3DQ8; -.
DR PDBsum; 3DQ9; -.
DR PDBsum; 3DQA; -.
DR PDBsum; 3DQC; -.
DR PDBsum; 3DQD; -.
DR PDBsum; 3DQE; -.
DR PDBsum; 3DQF; -.
DR PDBsum; 3DQH; -.
DR PDBsum; 3DQI; -.
DR PDBsum; 3DQJ; -.
DR PDBsum; 3DQK; -.
DR PDBsum; 3DQL; -.
DR PDBsum; 3DQM; -.
DR PDBsum; 3DQN; -.
DR PDBsum; 3DQO; -.
DR PDBsum; 3DQU; -.
DR PDBsum; 3ED8; -.
DR PDBsum; 3EK4; -.
DR PDBsum; 3EK7; -.
DR PDBsum; 3EK8; -.
DR PDBsum; 3EKH; -.
DR PDBsum; 3EKJ; -.
DR PDBsum; 3EVP; -.
DR PDBsum; 3EVR; -.
DR PDBsum; 3EVU; -.
DR PDBsum; 3EVV; -.
DR PDBsum; 3G9A; -.
DR PDBsum; 3GEX; -.
DR PDBsum; 3GJ1; -.
DR PDBsum; 3GJ2; -.
DR PDBsum; 3I19; -.
DR PDBsum; 3K1K; -.
DR PDBsum; 3LA1; -.
DR PDBsum; 3O77; -.
DR PDBsum; 3O78; -.
DR PDBsum; 3OGO; -.
DR PDBsum; 3OSQ; -.
DR PDBsum; 3OSR; -.
DR PDBsum; 3P28; -.
DR PDBsum; 3SG2; -.
DR PDBsum; 3SG3; -.
DR PDBsum; 3SG4; -.
DR PDBsum; 3SG5; -.
DR PDBsum; 3SG6; -.
DR PDBsum; 3SG7; -.
DR PDBsum; 3SRY; -.
DR PDBsum; 3SS0; -.
DR PDBsum; 3SSH; -.
DR PDBsum; 3SSK; -.
DR PDBsum; 3SSL; -.
DR PDBsum; 3SSP; -.
DR PDBsum; 3SST; -.
DR PDBsum; 3SSV; -.
DR PDBsum; 3SSY; -.
DR PDBsum; 3ST0; -.
DR PDBsum; 3SV5; -.
DR PDBsum; 3SVB; -.
DR PDBsum; 3SVC; -.
DR PDBsum; 3SVD; -.
DR PDBsum; 3SVE; -.
DR PDBsum; 3U8P; -.
DR PDBsum; 3UFZ; -.
DR PDBsum; 3UG0; -.
DR PDBsum; 3V3D; -.
DR PDBsum; 3VHT; -.
DR PDBsum; 3W1C; -.
DR PDBsum; 3W1D; -.
DR PDBsum; 3WLC; -.
DR PDBsum; 3WLD; -.
DR PDBsum; 3ZTF; -.
DR PDBsum; 4ANJ; -.
DR PDBsum; 4AR7; -.
DR PDBsum; 4AS8; -.
DR PDBsum; 4B5Y; -.
DR PDBsum; 4BDU; -.
DR PDBsum; 4EN1; -.
DR PDBsum; 4EUL; -.
DR PDBsum; 4GES; -.
DR PDBsum; 4GF6; -.
DR PDBsum; 4H47; -.
DR PDBsum; 4H48; -.
DR PDBsum; 4IK1; -.
DR PDBsum; 4IK3; -.
DR PDBsum; 4IK4; -.
DR PDBsum; 4IK5; -.
DR PDBsum; 4IK8; -.
DR PDBsum; 4IK9; -.
DR PDBsum; 4J88; -.
DR PDBsum; 4J89; -.
DR PDBsum; 4J8A; -.
DR PDBsum; 4JFG; -.
DR PDBsum; 4JRB; -.
DR PDBsum; 4KA9; -.
DR PDBsum; 4KAG; -.
DR PDBsum; 4KEX; -.
DR PDBsum; 4KF5; -.
DR PDBsum; 4KW4; -.
DR PDBsum; 4KW8; -.
DR PDBsum; 4KW9; -.
DR PDBsum; 4L12; -.
DR PDBsum; 4L13; -.
DR PDBsum; 4L1I; -.
DR PDBsum; 4LQT; -.
DR PDBsum; 4LQU; -.
DR PDBsum; 4LW5; -.
DR PDBsum; 4N3D; -.
DR PDBsum; 4NDJ; -.
DR PDBsum; 4NDK; -.
DR PDBsum; 4OGS; -.
DR PDBsum; 4ORN; -.
DR PDBsum; 4P1Q; -.
DR PDBsum; 4P7H; -.
DR PDBsum; 4PA0; -.
DR PDBsum; 4PFE; -.
DR PDBsum; 4U2V; -.
DR PDBsum; 4XBI; -.
DR PDBsum; 4XGY; -.
DR PDBsum; 4XL5; -.
DR PDBsum; 4XOV; -.
DR PDBsum; 4XOW; -.
DR PDBsum; 4XVP; -.
DR PDBsum; 4Z4K; -.
DR PDBsum; 4Z4M; -.
DR PDBsum; 4ZF3; -.
DR PDBsum; 4ZF4; -.
DR PDBsum; 4ZF5; -.
DR PDBsum; 5AQB; -.
DR PDBsum; 5B61; -.
DR PDBsum; 5BKF; -.
DR PDBsum; 5BKG; -.
DR PDBsum; 5DPG; -.
DR PDBsum; 5DPH; -.
DR PDBsum; 5DPI; -.
DR PDBsum; 5DPJ; -.
DR PDBsum; 5DQB; -.
DR PDBsum; 5DQM; -.
DR PDBsum; 5DRF; -.
DR PDBsum; 5DRG; -.
DR PDBsum; 5DTX; -.
DR PDBsum; 5DTY; -.
DR PDBsum; 5DTZ; -.
DR PDBsum; 5DU0; -.
DR PDBsum; 5EHU; -.
DR PDBsum; 5F9G; -.
DR PDBsum; 5FGU; -.
DR PDBsum; 5HBD; -.
DR PDBsum; 5HGE; -.
DR PDBsum; 5HW9; -.
DR PDBsum; 5HZO; -.
DR PDBsum; 5J2O; -.
DR PDBsum; 5J3N; -.
DR PDBsum; 5JZK; -.
DR PDBsum; 5JZL; -.
DR PDBsum; 5KTG; -.
DR PDBsum; 5LEL; -.
DR PDBsum; 5LEM; -.
DR PDBsum; 5MA3; -.
DR PDBsum; 5MA4; -.
DR PDBsum; 5MA5; -.
DR PDBsum; 5MA6; -.
DR PDBsum; 5MA8; -.
DR PDBsum; 5MA9; -.
DR PDBsum; 5MAD; -.
DR PDBsum; 5MAK; -.
DR PDBsum; 5MSE; -.
DR PDBsum; 5N9O; -.
DR PDBsum; 5NHN; -.
DR PDBsum; 5NI3; -.
DR PDBsum; 5O89; -.
DR PDBsum; 5O8B; -.
DR PDBsum; 5O8C; -.
DR PDBsum; 5OX8; -.
DR PDBsum; 5OX9; -.
DR PDBsum; 5OXA; -.
DR PDBsum; 5OXB; -.
DR PDBsum; 5OXC; -.
DR PDBsum; 5T3I; -.
DR PDBsum; 5WJ2; -.
DR PDBsum; 5WJ3; -.
DR PDBsum; 5WJ4; -.
DR PDBsum; 5Z6Y; -.
DR PDBsum; 6AA2; -.
DR PDBsum; 6AA6; -.
DR PDBsum; 6AS9; -.
DR PDBsum; 6B7R; -.
DR PDBsum; 6B7T; -.
DR PDBsum; 6B9C; -.
DR PDBsum; 6DQ0; -.
DR PDBsum; 6DQ1; -.
DR PDBsum; 6EFR; -.
DR PDBsum; 6FLL; -.
DR PDBsum; 6FWW; -.
DR PDBsum; 6GEL; -.
DR PDBsum; 6GEZ; -.
DR PDBsum; 6GO8; -.
DR PDBsum; 6GO9; -.
DR PDBsum; 6GQG; -.
DR PDBsum; 6GQH; -.
DR PDBsum; 6GRM; -.
DR PDBsum; 6HR1; -.
DR PDBsum; 6HUT; -.
DR PDBsum; 6IR6; -.
DR PDBsum; 6IR7; -.
DR PDBsum; 6ITC; -.
DR PDBsum; 6JGH; -.
DR PDBsum; 6JGI; -.
DR PDBsum; 6JGJ; -.
DR PDBsum; 6KKZ; -.
DR PDBsum; 6KL0; -.
DR PDBsum; 6KL1; -.
DR PDBsum; 6KRG; -.
DR PDBsum; 6L27; -.
DR PDBsum; 6LNP; -.
DR PDBsum; 6LR7; -.
DR PDBsum; 6MB2; -.
DR PDBsum; 6MDR; -.
DR PDBsum; 6MKS; -.
DR PDBsum; 6MWQ; -.
DR PDBsum; 6OA8; -.
DR PDBsum; 6OFK; -.
DR PDBsum; 6OFL; -.
DR PDBsum; 6OFM; -.
DR PDBsum; 6OFN; -.
DR PDBsum; 6OFO; -.
DR PDBsum; 6OG8; -.
DR PDBsum; 6OG9; -.
DR PDBsum; 6OGA; -.
DR PDBsum; 6OGB; -.
DR PDBsum; 6OGC; -.
DR PDBsum; 6PFR; -.
DR PDBsum; 6PFS; -.
DR PDBsum; 6PFT; -.
DR PDBsum; 6PFU; -.
DR PDBsum; 6QQ8; -.
DR PDBsum; 6QQ9; -.
DR PDBsum; 6QQA; -.
DR PDBsum; 6QQB; -.
DR PDBsum; 6QUH; -.
DR PDBsum; 6QUI; -.
DR PDBsum; 6QUJ; -.
DR PDBsum; 6SM0; -.
DR PDBsum; 6T39; -.
DR PDBsum; 6T3A; -.
DR PDBsum; 6T90; -.
DR PDBsum; 6UHJ; -.
DR PDBsum; 6UHK; -.
DR PDBsum; 6UHL; -.
DR PDBsum; 6UHM; -.
DR PDBsum; 6UHN; -.
DR PDBsum; 6UHO; -.
DR PDBsum; 6UHP; -.
DR PDBsum; 6UHQ; -.
DR PDBsum; 6UHR; -.
DR PDBsum; 6UN4; -.
DR PDBsum; 6UN5; -.
DR PDBsum; 6UN6; -.
DR PDBsum; 6UN7; -.
DR PDBsum; 6UZ0; -.
DR PDBsum; 6UZ3; -.
DR PDBsum; 6VAL; -.
DR PDBsum; 6VAM; -.
DR PDBsum; 6VIO; -.
DR PDBsum; 6WRF; -.
DR PDBsum; 6WSG; -.
DR PDBsum; 6WV3; -.
DR PDBsum; 6WV4; -.
DR PDBsum; 6WV5; -.
DR PDBsum; 6WV6; -.
DR PDBsum; 6WV7; -.
DR PDBsum; 6WV8; -.
DR PDBsum; 6WV9; -.
DR PDBsum; 6WVA; -.
DR PDBsum; 6WVB; -.
DR PDBsum; 6WVD; -.
DR PDBsum; 6WVE; -.
DR PDBsum; 6WVF; -.
DR PDBsum; 6WVG; -.
DR PDBsum; 6WVH; -.
DR PDBsum; 6WVI; -.
DR PDBsum; 6XZF; -.
DR PDBsum; 6YOV; -.
DR PDBsum; 6ZUI; -.
DR PDBsum; 7A7K; -.
DR PDBsum; 7A7L; -.
DR PDBsum; 7AA5; -.
DR PDBsum; 7BWN; -.
DR PDBsum; 7BYL; -.
DR PDBsum; 7BYM; -.
DR PDBsum; 7BYN; -.
DR PDBsum; 7CD7; -.
DR PDBsum; 7CD8; -.
DR PDBsum; 7EDJ; -.
DR PDBsum; 7K18; -.
DR PDBsum; 7KM4; -.
DR PDBsum; 7KS0; -.
DR PDBsum; 7KS3; -.
DR PDBsum; 7KUY; -.
DR PDBsum; 7L31; -.
DR PDBsum; 7PCA; -.
DR PDBsum; 7PCZ; -.
DR PDBsum; 7PD0; -.
DR PDBsum; 7PNN; -.
DR AlphaFoldDB; P42212; -.
DR BMRB; P42212; -.
DR SMR; P42212; -.
DR IntAct; P42212; 1.
DR PRIDE; P42212; -.
DR ABCD; P42212; 70 sequenced antibodies.
DR BioCyc; MetaCyc:MON-20291; -.
DR EvolutionaryTrace; P42212; -.
DR GO; GO:0008218; P:bioluminescence; TAS:UniProtKB.
DR GO; GO:0006091; P:generation of precursor metabolites and energy; TAS:UniProtKB.
DR Gene3D; 2.40.155.10; -; 2.
DR InterPro; IPR009017; GFP.
DR InterPro; IPR011584; GFP-related.
DR InterPro; IPR000786; Green_fluorescent_prot.
DR Pfam; PF01353; GFP; 1.
DR PRINTS; PR01229; GFLUORESCENT.
DR SUPFAM; SSF54511; SSF54511; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromophore; Direct protein sequencing; Luminescence;
KW Photoprotein.
FT CHAIN 1..238
FT /note="Green fluorescent protein"
FT /id="PRO_0000192576"
FT MOD_RES 66
FT /note="(Z)-2,3-didehydrotyrosine"
FT /evidence="ECO:0000269|PubMed:8448132"
FT CROSSLNK 65..67
FT /note="5-imidazolinone (Ser-Gly)"
FT /evidence="ECO:0000269|PubMed:8448132"
FT VARIANT 100
FT /note="F -> Y"
FT /evidence="ECO:0000269|PubMed:1347277"
FT VARIANT 108
FT /note="T -> S"
FT /evidence="ECO:0000269|PubMed:1347277"
FT VARIANT 141
FT /note="L -> M"
FT /evidence="ECO:0000269|PubMed:1347277"
FT VARIANT 219
FT /note="V -> I"
FT /evidence="ECO:0000269|PubMed:1347277"
FT MUTAGEN 30
FT /note="S->R: In mut1.28; shifts fluorescence lifetime from
FT 3.03 to 2.76 ns; when associated with H-145. In mut2.2;
FT shifts fluorescence lifetime from 3.03 to 1.94 ns; when
FT associated with H-69 and H-145. In mut3.3; shifts
FT fluorescence lifetime from 3.03 to 1.88 ns; when associated
FT with L-46; H-69 and H-145. In EBFP1.2; shifts the
FT excitation and emission spectra to shorter wavelengths and
FT increases quantum yields compared to BFP; when associated
FT with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-
FT 206. In EBFP2.0; shifts the excitation and emission spectra
FT to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with N-39; H-66; A-72; T-
FT 105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-
FT 224."
FT /evidence="ECO:0000269|PubMed:16201762,
FT ECO:0000269|PubMed:22891786"
FT MUTAGEN 39
FT /note="Y->N: In EBFP1.2; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; H-66; A-72; T-
FT 105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the
FT excitation and emission spectra to shorter wavelengths and
FT increases quantum yields compared to BFP; when associated
FT with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155;
FT V-171; S-198; V-206 and V-224."
FT /evidence="ECO:0000269|PubMed:22891786"
FT MUTAGEN 46
FT /note="F->L: In mut3.3; shifts fluorescence lifetime from
FT 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145.
FT In R10-3; matures to the red-emitting state with excitation
FT and emission maxima at 555 and 585 nm, respectively; when
FT associated with L-64; N-68; Q-162; A-163; V-167 and L-171.
FT In Venus; leads to yellow fluorescence, improved maturation
FT and reduced environmental sensitivity; when associated with
FT L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203."
FT /evidence="ECO:0000269|PubMed:12370172,
FT ECO:0000269|PubMed:16201762, ECO:0000269|PubMed:18366185"
FT MUTAGEN 64
FT /note="F->L: In EGFP; increases fluoresence at warmer
FT temperatures such as 37 degrees Celsius; when associated
FT with T-65. In EBFP; gives rise to variants with blue
FT fluorescence; when associated with T-65 and H-66. In ECFP;
FT leads to cyan fluorescence, folds faster and more
FT efficiently at 37 degrees Celsius and has superior
FT solubility and brightness; when associated with T-65; W-66;
FT I-146; T-153 and A-163. In Cerulean; leads to improved
FT quantum yield, a higher extinction coefficient and is 2.5-
FT fold brighterH than ECFP; when associated with T-65; W-66;
FT A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3;
FT matures to the red-emitting state with excitation and
FT emission maxima at 555 and 585 nm, respectively; when
FT associated with L-46; N-68; Q-162; A-163; V-167 and L-171.
FT In GFPmut 1; red-shifts by about 100 nm the excitation
FT maxima, permitting efficient excitation at 488 nm and
FT increases fluorescence; when associated with T-65. In
FT VisGreen; leads to brighter fluorescence; when associated
FT with T-65; A-72; K-149 and T-167. In Venus; leads to yellow
FT fluorescence, improved maturation and reduced environmental
FT sensitivity; when associated with L-46; G-65; L-68; A-72;
FT T-153; A-163; G-175 and Y-203."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:11776264, ECO:0000269|PubMed:12370172,
FT ECO:0000269|PubMed:14990965, ECO:0000269|PubMed:15065907,
FT ECO:0000269|PubMed:17658219, ECO:0000269|PubMed:18366185,
FT ECO:0000269|PubMed:8707053, ECO:0000269|PubMed:9145105,
FT ECO:0000269|PubMed:9370472"
FT MUTAGEN 64
FT /note="F->M: In RSGFP4; increases fluorescence and shifts
FT the major exitation peak to 489-490 nm; when associated
FT with G-65 and L-69."
FT /evidence="ECO:0000269|PubMed:9634755"
FT MUTAGEN 65..66
FT /note="SY->TW: Gives rise to variants with cerulean
FT fluorsecence."
FT /evidence="ECO:0000269|PubMed:17685554"
FT MUTAGEN 65
FT /note="S->A: In GFPmut 2; red-shifts by about 100 nm the
FT excitation maxima, permitting efficient excitation at 488
FT nm and increases fluorescence; when associated with L-68
FT and A-72."
FT /evidence="ECO:0000269|PubMed:8707053"
FT MUTAGEN 65
FT /note="S->G: In EYFP; leads to yellow fluorescence, folds
FT faster and more efficiently at 37 degrees Celsius and has
FT superior solubility and brightness; when associated with L-
FT 68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant
FT when excited at 488 nm; when associated with A-72. Highly
FT fluorescent; when associated with Y-203; L-68 and A-72. In
FT RSGFP4; increases fluoresence and shifts the major
FT exitation peak to 489-490 nm; when associated with M-64 and
FT L-69. In YFP 10C; shifts the major emission and exitation
FT peak up to 20 nm; when associated with L-68; A-72 and Y-
FT 203. In Topaz; shifts the major emission and exitation peak
FT up to 20 nm; when associated with A-72; R-79 and Y-203. In
FT Venus; leads to yellow fluorescence, improved maturation
FT and reduced environmental sensitivity; when associated with
FT L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:12370172, ECO:0000269|PubMed:8707053,
FT ECO:0000269|PubMed:9634755, ECO:0000269|PubMed:9759496,
FT ECO:0000269|PubMed:9782051"
FT MUTAGEN 65
FT /note="S->T: Increases fluoresence, photostability and
FT shifts the major exitation peak to 488 nm. In EGFP;
FT increases fluoresence at warmer temperatures such as 37
FT degrees Celsius; when associated with L-64. In EBFP; gives
FT rise to variants with blue fluorescence; when associated
FT with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm
FT the excitation maxima, permitting efficient excitation at
FT 488 nm and increases fluorescence; when associated with T-
FT 65. In ECFP; leads to cyan fluorescence, folds faster and
FT more efficiently at 37 degrees Celsius and has superior
FT solubility and brightness; when associated with L-64; W-66;
FT I-146; T-153 and A-163. In Cerulean; leads to improved
FT quantum yield, a higher extinction coefficient and is 2.5-
FT fold brighter than ECFP; when associated with L-64; W-66;
FT A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald;
FT leads to brighter fluorescence; when associated with A-72;
FT K-149; T-153 and T-167. In VisGreen; leads to brighter
FT fluorescence; when associated with L-64; A-72; K-149 and T-
FT 167."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:11776264, ECO:0000269|PubMed:14990965,
FT ECO:0000269|PubMed:15065907, ECO:0000269|PubMed:17658219,
FT ECO:0000269|PubMed:8703075, ECO:0000269|PubMed:8707053,
FT ECO:0000269|PubMed:9370472"
FT MUTAGEN 66
FT /note="Y->H: In BFP; shifts the excitation and emission
FT spectra to shorter wavelengths. In EBFP; gives rise to
FT variants with blue fluorescence; when associated with L-64
FT and T-65. In Azurite; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-80; F-145; I-150
FT and R-224. In EBFP1.2; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; A-72; T-
FT 105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the
FT excitation and emission spectra to shorter wavelengths and
FT increases quantum yields compared to BFP; when associated
FT with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155;
FT V-171; S-198; V-206 and V-224."
FT /evidence="ECO:0000269|PubMed:22891786,
FT ECO:0000269|PubMed:8578587, ECO:0000269|PubMed:9145105,
FT ECO:0000269|PubMed:9370472"
FT MUTAGEN 66
FT /note="Y->T,F: Shifts the excitation and emission spectra
FT to shorter wavelengths."
FT /evidence="ECO:0000269|PubMed:8578587"
FT MUTAGEN 66
FT /note="Y->W: In W; leads to excitation and emission
FT wavelengths intermediate between tyrosine and histidine but
FT is only weakly fluorescent. In ECFP; leads to cyan
FT fluorescence, folds faster and more efficiently at 37
FT degrees Celsius and has superior solubility and brightness;
FT when associated with L-64; T-65; I-146; T-153 and A-163. In
FT Cerulean; leads to improved quantum yield, a higher
FT extinction coefficient and is 2.5-fold brighter than ECFP;
FT when associated with L-64; T-65; A-72; A-145; I-146; D-148;
FT T-153 and A-163."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:14990965, ECO:0000269|PubMed:15065907,
FT ECO:0000269|PubMed:7809066"
FT MUTAGEN 68
FT /note="V->L: In EYFP; leads to yellow fluorescence, folds
FT faster and more efficiently at 37 degrees Celsius and has
FT superior solubility and brightness; when associated with G-
FT 65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm
FT the excitation maxima, permitting efficient excitation at
FT 488 nm and increases fluorescence; when associated with A-
FT 65 and A-72. In Citrinine; leads to excitation and emission
FT peaks of 516 and 529 nm, respectively; when associated with
FT M-69; A-72 and Y-203. In YFP 10C; shifts the major emission
FT and exitation peak up to 20 nm; when associated with G-65;
FT A-72 and Y-203. In Venus; leads to yellow fluorescence,
FT improved maturation and reduced environmental sensitivity;
FT when associated with L-46; L-64; G-65; A-72; T-153; A-163;
FT G-175 and Y-203."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:11387331, ECO:0000269|PubMed:12370172,
FT ECO:0000269|PubMed:8707053, ECO:0000269|PubMed:9759496,
FT ECO:0000269|PubMed:9782051"
FT MUTAGEN 68
FT /note="V->N: In R10-3; matures to the red-emitting state
FT with excitation and emission maxima at 555 and 585 nm,
FT respectively; when associated with L-46; L-64; Q-162; A-
FT 163; V-167 and L-171."
FT /evidence="ECO:0000269|PubMed:18366185"
FT MUTAGEN 69
FT /note="Q->H: In P4; leads to no detectable fluorescence. In
FT mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns;
FT when associated with R-30 and H-145. In mut3.3; shifts
FT fluorescence lifetime from 3.03 to 1.88 ns; when associated
FT with R-30; L-46 and H-145."
FT /evidence="ECO:0000269|PubMed:16201762,
FT ECO:0000269|PubMed:7809066"
FT MUTAGEN 69
FT /note="Q->L: In RSGFP4; increases fluorescence and shifts
FT the major exitation peak to 489-490 nm; when associated
FT with M-64 and G-65."
FT /evidence="ECO:0000269|PubMed:9634755"
FT MUTAGEN 69
FT /note="Q->M: In Citrinine; leads to excitation and emission
FT peaks of 516 and 529 nm, respectively; when associated with
FT L-68; A-72 and Y-203."
FT /evidence="ECO:0000269|PubMed:11387331"
FT MUTAGEN 72
FT /note="S->A: Increases fluoresence at warmer temperatures
FT such as 37 degrees Celsius. In GFPmut 3; highly fluorescent
FT mutant when excited at 488 nm; when associated with G-65.
FT In EYFP; leads to yellow fluorescence, folds faster and
FT more efficiently at 37 degrees Celsius and has superior
FT solubility and brightness; when associated with G-65; L-68
FT and Y-203. In GFPmut 2; red-shifts by about 100 nm the
FT excitation maxima, permitting efficient excitation at 488
FT nm and increases fluorescence; when associated with A-65
FT and L-68. In Citrinine; leads to excitation and emission
FT peaks of 516 and 529 nm, respectively; when associated with
FT L-68; M-69 and Y-203. In YFP 10C; shifts the major emission
FT and exitation peak up to 20 nm; when associated with G-65;
FT L-68; and Y-203. In Topaz; shifts the major emission and
FT exitation peak up to 20 nm; when associated with G-65; R-79
FT and Y-203. In Cerulean; leads to improved quantum yield, a
FT higher extinction coefficient and is 2.5-fold brighter than
FT ECFP; when associated with L-64; T-65; W-66; A-145; I-146;
FT D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge
FT Stoke's shift, with an excitation peak at 399 nm and an
FT emission peak at 511 nm; when associated with F-145 and I-
FT 203. In EBFP1.2; shifts the excitation and emission spectra
FT to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; H-66; T-
FT 105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the
FT excitation and emission spectra to shorter wavelengths and
FT increases quantum yields compared to BFP; when associated
FT with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155;
FT V-171; S-198; V-206 and V-224. In Esmerald; leads to
FT brighter fluorescence; when associated with T-65; K-149; T-
FT 153 and T-167. In VisGreen; leads to brighter fluorescence;
FT when associated with L-64; T-65; K-149 and T-167. In Venus;
FT leads to yellow fluorescence, improved maturation and
FT reduced environmental sensitivity; when associated with L-
FT 46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:11387331, ECO:0000269|PubMed:12370172,
FT ECO:0000269|PubMed:12769828, ECO:0000269|PubMed:14990965,
FT ECO:0000269|PubMed:17658219, ECO:0000269|PubMed:22891786,
FT ECO:0000269|PubMed:8707053, ECO:0000269|PubMed:9145105,
FT ECO:0000269|PubMed:9759496, ECO:0000269|PubMed:9782051"
FT MUTAGEN 79
FT /note="K->R: In Topaz; shifts the major emission and
FT exitation peak up to 20 nm; when associated with G-65; A-72
FT and Y-203."
FT /evidence="ECO:0000269|PubMed:9759496"
FT MUTAGEN 80
FT /note="Q->R: In Azurite; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with H-66; F-145; I-150
FT and R-224."
FT /evidence="ECO:0000269|PubMed:22891786"
FT MUTAGEN 99
FT /note="F->S: In alphaGFP/cycle 3 GFP; improves folding at
FT 37 degrees Celsius, reduces aggregation at high
FT concentrations, and increases the diffusibility of the
FT protein inside cells; when associated with T-153 and A-
FT 163."
FT /evidence="ECO:0000269|PubMed:9370472,
FT ECO:0000269|PubMed:9630892, ECO:0000269|PubMed:9631088"
FT MUTAGEN 103
FT /note="D->E: In mut1.27; shifts fluorescence lifetime from
FT 3.03 to 2.85 ns; when associated with H-145."
FT /evidence="ECO:0000269|PubMed:16201762"
FT MUTAGEN 105
FT /note="N->T: In EBFP1.2; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; H-66; A-
FT 72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the
FT excitation and emission spectra to shorter wavelengths and
FT increases quantum yields compared to BFP; when associated
FT with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-
FT 171; S-198; V-206 and V-224."
FT /evidence="ECO:0000269|PubMed:22891786"
FT MUTAGEN 128
FT /note="I->V: In EBFP2.0; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; H-66; A-
FT 72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-
FT 224."
FT /evidence="ECO:0000269|PubMed:22891786"
FT MUTAGEN 145
FT /note="Y->A: In Cerulean; leads to improved quantum yield,
FT a higher extinction coefficient and is 2.5-fold brighter
FT than ECFP; when associated with L-64; T-65; W-66; A-72; I-
FT 146; D-148; T-153 and A-163."
FT /evidence="ECO:0000269|PubMed:14990965"
FT MUTAGEN 145
FT /note="Y->C: In mut1.9; shifts fluorescence lifetime from
FT 3.03 to 2.74 ns."
FT /evidence="ECO:0000269|PubMed:16201762"
FT MUTAGEN 145
FT /note="Y->F: In Sapphire/H9-40; exhibits a huge Stoke's
FT shift, with an excitation peak at 399 nm and an emission
FT peak at 511 nm; when associated with A-72 and I-203. In
FT Azurite; shifts the excitation and emission spectra to
FT shorter wavelengths and increases quantum yields compared
FT to BFP; when associated with H-66; R-80; I-150 and R-224.
FT In EBFP1.2; shifts the excitation and emission spectra to
FT shorter wavelengths and increases quantum yields compared
FT to BFP; when associated with R-30; N-39; H-66; A-72; T-105;
FT V-171; S-198 and V-206. In EBFP2.0; shifts the excitation
FT and emission spectra to shorter wavelengths and increases
FT quantum yields compared to BFP; when associated with R-30;
FT N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198;
FT V-206 and V-224."
FT /evidence="ECO:0000269|PubMed:12769828,
FT ECO:0000269|PubMed:22891786"
FT MUTAGEN 145
FT /note="Y->H: In mut1.3; shifts fluorescence lifetime from
FT 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime
FT from 3.03 to 2.72 ns; when associated with A-193. In
FT mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns;
FT when associated with E-103. In mut1.28; shifts fluorescence
FT lifetime from 3.03 to 2.76 ns; when associated with R-30.
FT In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50
FT ns; when associated with A-176 and I-198. In mut2.2; shifts
FT fluorescence lifetime from 3.03 to 1.94 ns; when associated
FT with R-30 and H-69. In mut3.3; shifts fluorescence lifetime
FT from 3.03 to 1.88 ns; when associated with R-30; L-46 and
FT H-69."
FT /evidence="ECO:0000269|PubMed:16201762"
FT MUTAGEN 146
FT /note="N->I: In ECFP; leads to cyan fluorescence, folds
FT faster and more efficiently at 37 degrees Celsius and has
FT superior solubility and brightness; when associated with L-
FT 64; T-65; W-66; T-153 and A-163. In Cerulean; leads to
FT improved quantum yield, a higher extinction coefficient and
FT is 2.5-fold brighter than ECFP; when associated with L-64;
FT T-65; W-66; A-72; A-145; D-148; T-153 and A-163."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:14990965, ECO:0000269|PubMed:15065907"
FT MUTAGEN 147
FT /note="S->P: Increases fluorescence at warmer temperatures
FT such as 37 degrees Celsius."
FT /evidence="ECO:0000269|PubMed:9125154"
FT MUTAGEN 148
FT /note="H->D: In Cerulean; leads to improved quantum yield,
FT a higher extinction coefficient and is 2.5-fold brighter
FT than ECFP; when associated with L-64; T-65; W-66; A-72; A-
FT 145; I-146; T-153 and A-163."
FT /evidence="ECO:0000269|PubMed:14990965"
FT MUTAGEN 149
FT /note="N->K: Increases fluorescence at warmer temperatures
FT such as 37 degrees Celsius. In Esmerald; leads to brighter
FT fluorescence; when associated with T-65; A-72; T-153 and T-
FT 167. In VisGreen; leads to brighter fluorescence; when
FT associated with L-64; T-65; A-72 and T-167."
FT /evidence="ECO:0000269|PubMed:17658219,
FT ECO:0000269|PubMed:9145105"
FT MUTAGEN 150
FT /note="V->I: In Azurite; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with H-66; R-80; F-145 and
FT R-224. In EBFP2.0; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; H-66; A-
FT 72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-
FT 224."
FT /evidence="ECO:0000269|PubMed:22891786"
FT MUTAGEN 153
FT /note="M->T: Increases fluorescence at warmer temperatures
FT such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP;
FT improves folding at 37 degrees Celsius, reduces aggregation
FT at high concentrations, and increases the diffusibility of
FT the protein inside cells; when associated with S-99 and A-
FT 163. In ECFP; leads to cyan fluorescence, folds faster and
FT more efficiently at 37 degrees Celsius and has superior
FT solubility and brightness; when associated with L-64; T-65;
FT W-66; I-146 and A-163. In Cerulean; leads to improved
FT quantum yield, a higher extinction coefficient and is 2.5-
FT fold brighter than ECFP; when associated with L-64; T-65;
FT W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald;
FT leads to brighter fluorescence; when associated with T-65;
FT A-72; K-149 and T-167. In Venus; leads to yellow
FT fluorescence, improved maturation and reduced environmental
FT sensitivity; when associated with L-46; L-64; G-65; L-68;
FT A-72; A-163; G-175 and Y-203."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:12370172, ECO:0000269|PubMed:14990965,
FT ECO:0000269|PubMed:15065907, ECO:0000269|PubMed:17658219,
FT ECO:0000269|PubMed:9145105, ECO:0000269|PubMed:9370472,
FT ECO:0000269|PubMed:9630892, ECO:0000269|PubMed:9631088"
FT MUTAGEN 155
FT /note="D->V: In EBFP2.0; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; H-66; A-
FT 72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-
FT 224."
FT /evidence="ECO:0000269|PubMed:22891786"
FT MUTAGEN 162
FT /note="K->Q: In R10-3; matures to the red-emitting state
FT with excitation and emission maxima at 555 and 585 nm,
FT respectively; when associated with L-46; L-64; N-68; A-163;
FT V-167 and L-171."
FT /evidence="ECO:0000269|PubMed:18366185"
FT MUTAGEN 163
FT /note="V->A: In GFPB; leads to enhanced fluorescence at 37
FT degrees Celsius. In GFPA; leads to even higher fluorescence
FT at 37 degrees Celsius than GFPA; whenassociated with G-175.
FT In alphaGFP/cycle 3 GFP; improves folding at 37 degrees
FT Celsius, reduces aggregation at high concentrations, and
FT increases the diffusibility of the protein inside cells;
FT when associated with S-99 and T-153. In R10-3; matures to
FT the red-emitting state with excitation and emission maxima
FT at 555 and 585 nm, respectively; when associated with L-46;
FT L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan
FT fluorescence, folds faster and more efficiently at 37
FT degrees Celsius and has superior solubility and brightness;
FT when associated with L-64; T-65; W-66; I-146 and T-153. In
FT Cerulean; leads to improved quantum yield, a higher
FT extinction coefficient and is 2.5-fold brighter than ECFP;
FT when associated with L-64; T-65; W-66; A-72; A-145; I-146;
FT D-148 and T-153. In Venus; leads to yellow fluorescence,
FT improved maturation and reduced environmental sensitivity;
FT when associated with L-46; L-64; G-65; L-68; A-72; T-153;
FT G-175 and Y-203."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:12370172, ECO:0000269|PubMed:14990965,
FT ECO:0000269|PubMed:15065907, ECO:0000269|PubMed:18366185,
FT ECO:0000269|PubMed:8994830, ECO:0000269|PubMed:9370472,
FT ECO:0000269|PubMed:9630892, ECO:0000269|PubMed:9631088"
FT MUTAGEN 167
FT /note="I->T: In P11; increases fluorescence at 475 nm
FT excitation and at warmer temperatures such as 37 degrees
FT Celsius. In Esmerald; leads to brighter fluorescence; when
FT associated with T-65; A-72; K-149 and T-153. In VisGreen;
FT leads to brighter fluorescence; when associated with L-64;
FT T-65; A-72 and K-149."
FT /evidence="ECO:0000269|PubMed:17658219,
FT ECO:0000269|PubMed:7809066, ECO:0000269|PubMed:9145105"
FT MUTAGEN 167
FT /note="I->V: In P9; increases fluorescence at 475 nm
FT excitation. In R10-3; matures to the red-emitting state
FT with excitation and emission maxima at 555 and 585 nm,
FT respectively; when associated with L-46; L-64; N-68; Q-162;
FT A-163 and L-171."
FT /evidence="ECO:0000269|PubMed:18366185,
FT ECO:0000269|PubMed:7809066"
FT MUTAGEN 171
FT /note="I->L: In R10-3; matures to the red-emitting state
FT with excitation and emission maxima at 555 and 585 nm,
FT respectively; when associated with L-46; L-64; N-68; Q-162;
FT A-163 and V-167."
FT /evidence="ECO:0000269|PubMed:18366185"
FT MUTAGEN 171
FT /note="I->V: In EBFP1.2; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; H-66; A-
FT 72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the
FT excitation and emission spectra to shorter wavelengths and
FT increases quantum yields compared to BFP; when associated
FT with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-
FT 155; S-198; V-206 and V-224."
FT /evidence="ECO:0000269|PubMed:22891786"
FT MUTAGEN 175
FT /note="S->G: In GFPA; leads to enhanced fluorescence at 37
FT degrees Celsius; when associated with A-163. In Venus;
FT leads to yellow fluorescence, improved maturation and
FT reduced environmental sensitivity; when associated with L-
FT 46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203."
FT /evidence="ECO:0000269|PubMed:12370172,
FT ECO:0000269|PubMed:8994830"
FT MUTAGEN 176
FT /note="V->A: In mut2.1; shifts fluorescence lifetime from
FT 3.03 to 2.50 ns; when associated with H-145 and I-198."
FT /evidence="ECO:0000269|PubMed:16201762"
FT MUTAGEN 193
FT /note="V->A: In mut1.5; shifts fluorescence lifetime from
FT 3.03 to 2.72 ns; when associated with H-145."
FT /evidence="ECO:0000269|PubMed:16201762"
FT MUTAGEN 198
FT /note="N->I: In mut2.1; shifts fluorescence lifetime from
FT 3.03 to 2.50 ns; when associated with H-145 and A-176."
FT /evidence="ECO:0000269|PubMed:16201762"
FT MUTAGEN 198
FT /note="N->S: In EBFP1.2; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; H-66; A-
FT 72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the
FT excitation and emission spectra to shorter wavelengths and
FT increases quantum yields compared to BFP; when associated
FT with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-
FT 155; V-171; S-198 and V-224."
FT /evidence="ECO:0000269|PubMed:22891786"
FT MUTAGEN 202
FT /note="S->F: In H9; increases fluorescence at 395 nm
FT excitation; when associated with I-203."
FT /evidence="ECO:0000269|PubMed:7809066"
FT MUTAGEN 203
FT /note="T->H,W,F: Results in significantly red-shifted
FT excitation and emission maxima."
FT /evidence="ECO:0000269|PubMed:8703075"
FT MUTAGEN 203
FT /note="T->I: Suppresses the 475 nm excitation peak, leaving
FT only the shorter wavelength peak at 399 nm. In H9;
FT increases fluorescence at 395 nm excitation; when
FT associated with I-203. In Sapphire/H9-40; exhibits a huge
FT Stoke's shift, with an excitation peak at 399 nm and an
FT emission peak at 511 nm; when associated with A-72 and F-
FT 145."
FT /evidence="ECO:0000269|PubMed:12769828,
FT ECO:0000269|PubMed:7796912, ECO:0000269|PubMed:7809066"
FT MUTAGEN 203
FT /note="T->Y: Gives rise to yellow-emission variants. In
FT EYFP; leads to yellow fluorescence, folds faster and more
FT efficiently at 37 degrees Celsius and has superior
FT solubility and brightness; when associated with G-65; L-68
FT and A-72. In Citrinine; leads to excitation and emission
FT peaks of 516 and 529 nm, respectively; when associated with
FT L-68; M-69 and A-72. In YFP 10C; shifts the major emission
FT and exitation peak up to 20 nm; when associated with G-65;
FT L-68 and A-72. In Topaz; shifts the major emission and
FT exitation peak up to 20 nm; when associated with G-65; A-72
FT and R-79. In Venus; leads to yellow fluorescence, improved
FT maturation and reduced environmental sensitivity; when
FT associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163
FT and G-175."
FT /evidence="ECO:0000269|PubMed:11299042,
FT ECO:0000269|PubMed:11387331, ECO:0000269|PubMed:12370172,
FT ECO:0000269|PubMed:9759496, ECO:0000269|PubMed:9782051"
FT MUTAGEN 206
FT /note="A->K: Abolishes the tendency to dimerize and leads
FT to monomeric fluorescent proteins."
FT /evidence="ECO:0000269|PubMed:16739159"
FT MUTAGEN 206
FT /note="A->V: In EBFP1.2; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; H-66; A-
FT 72; T-105; F-145; V-171 and S-198. In EBFP2.0; shifts the
FT excitation and emission spectra to shorter wavelengths and
FT increases quantum yields compared to BFP; when associated
FT with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-
FT 155; V-171; S-198 and V-206."
FT MUTAGEN 222
FT /note="E->G: Suppresses the 399 nm excitation peak, leaving
FT only the longer wavelength peak at 475 nm."
FT /evidence="ECO:0000269|PubMed:7796912"
FT MUTAGEN 224
FT /note="V->R: In Azurite; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with H-66; R-80; F-145 and
FT I-150. In EBFP2.0; shifts the excitation and emission
FT spectra to shorter wavelengths and increases quantum yields
FT compared to BFP; when associated with R-30; N-39; H-66; A-
FT 72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-
FT 206."
FT /evidence="ECO:0000269|PubMed:22891786"
FT CONFLICT 2
FT /note="S -> G (in Ref. 3; CAA65278)"
FT /evidence="ECO:0000305"
FT CONFLICT 25
FT /note="H -> Q (in Ref. 3; CAA65278)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="Q -> R (in Ref. 3; CAA65278)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="Q -> P (in Ref. 2; AAA58246)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="E -> K (in Ref. 2; AAA58246)"
FT /evidence="ECO:0000305"
FT TURN 1..3
FT /evidence="ECO:0007829|PDB:6UHQ"
FT HELIX 5..8
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 12..22
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 25..36
FT /evidence="ECO:0007829|PDB:6L27"
FT HELIX 37..39
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 41..48
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:5LEM"
FT HELIX 57..60
FT /evidence="ECO:0007829|PDB:6L27"
FT HELIX 61..64
FT /evidence="ECO:0007829|PDB:6L27"
FT TURN 65..67
FT /evidence="ECO:0007829|PDB:1QYO"
FT HELIX 69..71
FT /evidence="ECO:0007829|PDB:6L27"
FT HELIX 72..74
FT /evidence="ECO:0007829|PDB:6OA8"
FT HELIX 76..81
FT /evidence="ECO:0007829|PDB:6L27"
FT HELIX 83..86
FT /evidence="ECO:0007829|PDB:6L27"
FT TURN 87..90
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 92..100
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 101..103
FT /evidence="ECO:0007829|PDB:6MWQ"
FT STRAND 105..115
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 118..129
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 132..134
FT /evidence="ECO:0007829|PDB:1EMK"
FT TURN 135..139
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 141..147
FT /evidence="ECO:0007829|PDB:4ZF5"
FT STRAND 148..154
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 158..171
FT /evidence="ECO:0007829|PDB:6L27"
FT TURN 172..174
FT /evidence="ECO:0007829|PDB:4OGS"
FT STRAND 176..191
FT /evidence="ECO:0007829|PDB:6L27"
FT STRAND 198..208
FT /evidence="ECO:0007829|PDB:6L27"
FT HELIX 209..213
FT /evidence="ECO:0007829|PDB:4IK8"
FT STRAND 215..228
FT /evidence="ECO:0007829|PDB:6L27"
FT HELIX 233..236
FT /evidence="ECO:0007829|PDB:4GF6"
SQ SEQUENCE 238 AA; 26886 MW; EA5A6F21FBFB6E05 CRC64;
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL
VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV
NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD
HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
