ID   GFRA4_RAT               Reviewed;         273 AA.
AC   Q9EPI2; Q9EPI3;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-APR-2003, sequence version 2.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=GDNF family receptor alpha-4;
DE            Short=GDNF receptor alpha-4;
DE            Short=GDNFR-alpha-4;
DE            Short=GFR-alpha-4;
DE   AltName: Full=Persephin receptor;
DE   Flags: Precursor;
GN   Name=Gfra4;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), AND VARIANT ARG-257.
RC   TISSUE=Brain, Heart, and Kidney;
RX   PubMed=10958791; DOI=10.1074/jbc.m003867200;
RA   Masure S., Cik M., Hoefnagel E., Nosrat C.A., Van der Linden I., Scott R.,
RA   Van Gompel P., Lesage A.S.J., Verhasselt P., Ibanez C.F., Gordon R.D.;
RT   "Mammalian GFRalpha-4, a divergent member of the GFRalpha family of
RT   coreceptors for glial cell line-derived neurotrophic factor family ligands,
RT   is a receptor for the neurotrophic factor persephin.";
RL   J. Biol. Chem. 275:39427-39434(2000).
CC   -!- FUNCTION: Receptor for persephin. Mediates the GDNF-induced
CC       autophosphorylation and activation of the RET receptor. May be
CC       important in C-cell development and, in the postnatal development of
CC       the adrenal medulla.
CC   -!- SUBUNIT: Interacts with SORL1. {ECO:0000250|UniProtKB:Q9GZZ7}.
CC   -!- SUBCELLULAR LOCATION: [Isoform A]: Cell membrane; Lipid-anchor, GPI-
CC       anchor.
CC   -!- SUBCELLULAR LOCATION: [Isoform B]: Secreted {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC         Comment=Additional isoforms seem to exist.;
CC       Name=A;
CC         IsoId=Q9EPI2-1; Sequence=Displayed;
CC       Name=B;
CC         IsoId=Q9EPI2-2; Sequence=VSP_007230;
CC   -!- TISSUE SPECIFICITY: Weakly expressed in heart, brain and testis.
CC   -!- MISCELLANEOUS: [Isoform A]: GPI-anchored form.
CC   -!- SIMILARITY: Belongs to the GDNFR family. {ECO:0000305}.
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DR   EMBL; AJ294475; CAC16420.1; -; mRNA.
DR   EMBL; AJ294476; CAC16421.1; -; mRNA.
DR   RefSeq; NP_076457.1; NM_023967.1. [Q9EPI2-2]
DR   AlphaFoldDB; Q9EPI2; -.
DR   SMR; Q9EPI2; -.
DR   STRING; 10116.ENSRNOP00000042875; -.
DR   GlyGen; Q9EPI2; 1 site.
DR   PaxDb; Q9EPI2; -.
DR   Ensembl; ENSRNOT00000047947; ENSRNOP00000042875; ENSRNOG00000021241. [Q9EPI2-2]
DR   GeneID; 66023; -.
DR   KEGG; rno:66023; -.
DR   UCSC; RGD:620503; rat. [Q9EPI2-1]
DR   CTD; 64096; -.
DR   RGD; 620503; Gfra4.
DR   VEuPathDB; HostDB:ENSRNOG00000021241; -.
DR   eggNOG; ENOG502QSGA; Eukaryota.
DR   GeneTree; ENSGT00940000160491; -.
DR   HOGENOM; CLU_040179_2_0_1; -.
DR   InParanoid; Q9EPI2; -.
DR   OMA; AFDSGWP; -.
DR   OrthoDB; 1007489at2759; -.
DR   Reactome; R-RNO-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-RNO-8853659; RET signaling.
DR   PRO; PR:Q9EPI2; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   Bgee; ENSRNOG00000021241; Expressed in ovary and 15 other tissues.
DR   Genevisible; Q9EPI2; RN.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; IDA:RGD.
DR   GO; GO:0043235; C:receptor complex; IBA:GO_Central.
DR   GO; GO:0016167; F:glial cell-derived neurotrophic factor receptor activity; IDA:RGD.
DR   GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central.
DR   GO; GO:0030279; P:negative regulation of ossification; ISO:RGD.
DR   GO; GO:0007399; P:nervous system development; IBA:GO_Central.
DR   InterPro; IPR016017; GDNF/GAS1.
DR   InterPro; IPR037193; GDNF_alpha.
DR   InterPro; IPR003438; GDNF_rcpt.
DR   PANTHER; PTHR10269; PTHR10269; 1.
DR   Pfam; PF02351; GDNF; 2.
DR   PRINTS; PR01316; GDNFRECEPTOR.
DR   SMART; SM00907; GDNF; 2.
DR   SUPFAM; SSF110035; SSF110035; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein;
KW   Membrane; Receptor; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..?
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..250
FT                   /note="GDNF family receptor alpha-4"
FT                   /id="PRO_0000010797"
FT   PROPEP          251..273
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000010798"
FT   LIPID           250
FT                   /note="GPI-anchor amidated asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        192
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         253..273
FT                   /note="CCFLWVSSMSILTALALQALL -> QAKVEA (in isoform B)"
FT                   /evidence="ECO:0000303|PubMed:10958791"
FT                   /id="VSP_007230"
FT   VARIANT         257
FT                   /note="W -> R (in 50% of the molecules)"
FT                   /evidence="ECO:0000269|PubMed:10958791"
SQ   SEQUENCE   273 AA;  29682 MW;  E0BB76ABE2AC6B04 CRC64;
     MLSGAYLRVL NERPGQAVLW SLGCQRGSAS STEGNRCVEA AEACTADEQC QQLRSEYVAQ
     CLGRAGWRGP GSCVRSRCRR ALRRFFARGP PALTHALLFC GCEGPACAER RRQTFAPACA
     FSGPQLAPPS CLKPLDRCER SRRCRPRLFA FQASCAPAPG SRDGCPEEGG PRCLRAYAGL
     VGTVVTPNYL DNVSARVAPW CGCEASGNRR EECEAFRKLF TRNPCLDGAI QAFDSSQPSV
     LQDQWNPYQN AGCCFLWVSS MSILTALALQ ALL