GFY_MOUSE
ID GFY_MOUSE Reviewed; 504 AA.
AC J3KML8;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Golgi-associated olfactory signaling regulator;
DE AltName: Full=Golgi protein in olfactory neurons {ECO:0000303|PubMed:23926254};
DE Short=Goofy {ECO:0000303|PubMed:23926254};
DE Flags: Precursor;
GN Name=Gfy; Synonyms=Gm581, Goofy;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=23926254; DOI=10.1523/jneurosci.4948-12.2013;
RA Kaneko-Goto T., Sato Y., Katada S., Kinameri E., Yoshihara S.,
RA Nishiyori A., Kimura M., Fujita H., Touhara K., Reed R.R., Yoshihara Y.;
RT "Goofy coordinates the acuity of olfactory signaling.";
RL J. Neurosci. 33:12987-12996(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Required for proper function of the olfactory system. May be
CC involved in establishing the acuity of olfactory sensory signaling.
CC {ECO:0000269|PubMed:23926254}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:23926254}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:23926254}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in the olfactory epithelium
CC and vomeronasal organ throughout development. Expressed in both
CC immature and mature olfactory sensory neurons in the olfactory
CC epithelium (at protein level). {ECO:0000269|PubMed:23926254}.
CC -!- DEVELOPMENTAL STAGE: First expressed at embryonic day (E) 11.5 in the
CC olfactory epithelium and at 13.5 dpc in the vomeronasal organ.
CC Expression increases thereafter, reached maximal level at P14, and
CC strong expression level is maintained into adulthood.
CC {ECO:0000269|PubMed:23926254}.
CC -!- DISRUPTION PHENOTYPE: Various olfactory phenotypes, such as abnormal
CC localization of ADCY3, shortened olfactory cilia, and reduced
CC electrophysiological and behavioral sensitivities to odorants.
CC {ECO:0000269|PubMed:23926254}.
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DR EMBL; AB845286; BAN67991.1; -; mRNA.
DR EMBL; AC149868; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS57548.1; -.
DR RefSeq; NP_001182184.1; NM_001195255.1.
DR AlphaFoldDB; J3KML8; -.
DR SMR; J3KML8; -.
DR GlyGen; J3KML8; 1 site.
DR iPTMnet; J3KML8; -.
DR PhosphoSitePlus; J3KML8; -.
DR PaxDb; J3KML8; -.
DR PRIDE; J3KML8; -.
DR ProteomicsDB; 268871; -.
DR Ensembl; ENSMUST00000179443; ENSMUSP00000135927; ENSMUSG00000095276.
DR GeneID; 100039953; -.
DR KEGG; mmu:100039953; -.
DR UCSC; uc012fkd.1; mouse.
DR CTD; 100507003; -.
DR MGI; MGI:2685427; Gfy.
DR VEuPathDB; HostDB:ENSMUSG00000095276; -.
DR eggNOG; ENOG502RMAY; Eukaryota.
DR GeneTree; ENSGT00730000111841; -.
DR HOGENOM; CLU_040045_0_0_1; -.
DR InParanoid; J3KML8; -.
DR OMA; IHKPPKP; -.
DR OrthoDB; 795288at2759; -.
DR PhylomeDB; J3KML8; -.
DR BioGRID-ORCS; 100039953; 8 hits in 73 CRISPR screens.
DR PRO; PR:J3KML8; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; J3KML8; protein.
DR Bgee; ENSMUSG00000095276; Expressed in forelimb stylopod muscle and 40 other tissues.
DR ExpressionAtlas; J3KML8; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; IDA:MGI.
DR GO; GO:0030173; C:integral component of Golgi membrane; IDA:UniProtKB.
DR GO; GO:1905515; P:non-motile cilium assembly; IMP:MGI.
DR GO; GO:0097499; P:protein localization to non-motile cilium; IMP:MGI.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007608; P:sensory perception of smell; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Glycoprotein; Golgi apparatus; Membrane; Olfaction; Reference proteome;
KW Sensory transduction; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..504
FT /note="Golgi-associated olfactory signaling regulator"
FT /id="PRO_0000424824"
FT TOPO_DOM 23..397
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..504
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 36..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 467..504
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..155
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 172..187
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 196..295
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..325
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..355
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 468..490
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 504 AA; 53786 MW; 156177DDBC6C558D CRC64;
MQPFSPIFFH LLFLLNGLSS RAAPSPGQPV VADLQGMLQP SGMPTGTLEN LTRDQPTPGS
SASHPPEHSE TPPSASPHIS TKILRETPSP SPFLSLETPI PDQLTSVAES QGTSQMSPSR
ATLGKPSETP KPDPTGISPS DSPETPKPNP SNTSPPESPE SVYTDPTPTL HHESPEISKR
DTPKLSPGEE SKIPSPRPTQ FLSSKSLETY DPSATRHLNS ALEPTTHPDP TESPQSVFLT
THNSNPTVVP QTQFPTSPSQ NVTETARTSD LEPSSSLPTQ PTTFREEATT PSEPGLSPSP
EAPAVTRVAT PGLSTSDSPG TKELHVPQNS DPKGPDIPLP SARIAGPPAP LEHPNQVAPA
PQRHSRGDTV NTIIVVERVK ETGVTLVSRP RGSVGGALCL FFAGTGLLIG IFLLLWCLYR
RASRHRSFAH HRLRDSGDEP VLHLDAPKDP LDLYFYAPDA WVPSHIATQP PPSTPPLPPK
LPPPPRGPQR LEALSPAALS PNFF
