GGAG_MLVMN
ID GGAG_MLVMN Reviewed; 626 AA.
AC Q8UN02;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2015, sequence version 2.
DT 02-JUN-2021, entry version 87.
DE RecName: Full=Glyco-Gag protein;
DE AltName: Full=Gross cell surface antigen;
DE AltName: Full=glycosylated Pr80 gag;
DE Short=gPr80 Gag;
DE Short=gag-gPr80;
DE Contains:
DE RecName: Full=Nextended-MA-p12;
DE Contains:
DE RecName: Full=CA-NC;
GN Name=gag;
OS Moloney murine leukemia virus (strain neuropathogenic variant ts1-92b)
OS (MoMLV).
OC Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC Ortervirales; Retroviridae; Orthoretrovirinae; Gammaretrovirus;
OC Murine leukemia virus.
OX NCBI_TaxID=882209;
OH NCBI_TaxID=10090; Mus musculus (Mouse).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12881645; DOI=10.1023/a:1020996529014;
RA Szurek P.F., Vann J.M., Brooks B.R.;
RT "Sequence analysis of a neuropathogenic variant of Moloney murine leukemia
RT virus ts1: evidence for recombination.";
RL Virus Genes 25:343-344(2002).
RN [2]
RP FUNCTION, AND INITIATION ON LEUCINE.
RX PubMed=2538626; DOI=10.1016/0022-2836(89)90347-1;
RA Prats A.C., De Billy G., Wang P., Darlix J.L.;
RT "CUG initiation codon used for the synthesis of a cell surface antigen
RT coded by the murine leukemia virus.";
RL J. Mol. Biol. 205:363-372(1989).
RN [3]
RP FUNCTION.
RX PubMed=17267509; DOI=10.1128/jvi.01538-06;
RA Low A., Datta S., Kuznetsov Y., Jahid S., Kothari N., McPherson A., Fan H.;
RT "Mutation in the glycosylated gag protein of murine leukemia virus results
RT in reduced in vivo infectivity and a novel defect in viral budding or
RT release.";
RL J. Virol. 81:3685-3692(2007).
CC -!- FUNCTION: Seems to be important for efficient replication in vivo.
CC {ECO:0000269|PubMed:17267509, ECO:0000269|PubMed:2538626}.
CC -!- SUBCELLULAR LOCATION: [Nextended-MA-p12]: Host cell membrane
CC {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: [CA-NC]: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=Pr80gag; Synonyms=GlycoGag;
CC IsoId=Q8UN02-1; Sequence=Displayed;
CC Name=Pr65gag;
CC IsoId=Q8UN02-2; Sequence=Not described;
CC -!- PTM: May be cleaved within the capsid domain. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform Pr80gag]: Produced by an upstream CUG
CC initiation codon. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL69908.1; Type=Miscellaneous discrepancy; Evidence={ECO:0000305|PubMed:2538626};
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DR EMBL; AF462057; AAL69908.1; ALT_SEQ; Genomic_DNA.
DR PIR; S02769; S02769.
DR BMRB; Q8UN02; -.
DR SMR; Q8UN02; -.
DR SwissPalm; Q8UN02; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0019068; P:virion assembly; IEA:InterPro.
DR Gene3D; 1.10.150.180; -; 1.
DR Gene3D; 1.10.375.10; -; 1.
DR InterPro; IPR000840; G_retro_matrix.
DR InterPro; IPR036946; G_retro_matrix_sf.
DR InterPro; IPR002079; Gag_p12.
DR InterPro; IPR003036; Gag_P30.
DR InterPro; IPR008919; Retrov_capsid_N.
DR InterPro; IPR010999; Retrovr_matrix.
DR InterPro; IPR001878; Znf_CCHC.
DR InterPro; IPR036875; Znf_CCHC_sf.
DR Pfam; PF01140; Gag_MA; 1.
DR Pfam; PF01141; Gag_p12; 1.
DR Pfam; PF02093; Gag_p30; 1.
DR Pfam; PF00098; zf-CCHC; 1.
DR SMART; SM00343; ZnF_C2HC; 1.
DR SUPFAM; SSF47836; SSF47836; 1.
DR SUPFAM; SSF47943; SSF47943; 1.
DR SUPFAM; SSF57756; SSF57756; 1.
DR PROSITE; PS50158; ZF_CCHC; 1.
PE 3: Inferred from homology;
KW Alternative initiation; Coiled coil; Glycoprotein; Host cell membrane;
KW Host membrane; Membrane; Metal-binding; Phosphoprotein; Secreted;
KW Transmembrane; Transmembrane helix; Zinc; Zinc-finger.
FT CHAIN 1..626
FT /note="Glyco-Gag protein"
FT /id="PRO_0000390850"
FT CHAIN 1..?
FT /note="Nextended-MA-p12"
FT /evidence="ECO:0000255"
FT /id="PRO_5000061808"
FT CHAIN ?..626
FT /note="CA-NC"
FT /id="PRO_0000390851"
FT TRANSMEM 64..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ZN_FING 590..607
FT /note="CCHC-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT REGION 195..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 523..626
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 526..566
FT /evidence="ECO:0000255"
FT MOTIF 199..202
FT /note="PTAP/PSAP motif"
FT MOTIF 250..253
FT /note="PPXY motif"
FT MOTIF 383..385
FT /note="Cell attachment site"
FT /evidence="ECO:0000255"
FT COMPBIAS 195..209
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 523..564
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..611
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 280
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 113
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 505
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
SQ SEQUENCE 626 AA; 70485 MW; 9F16BAA64EDD4F10 CRC64;
LGDVPGTSGA VFVARPESKN PDRFGLFGAP PLEEGYVVLV GDENLKQFPP PSEFLLSVWN
RSRAARLVCC SIVLCCLCLT VFLYLSENMG QTVTTPLSLT LDHWKDVERI AHNQSVDVKK
RRWVTFCSAE WPTFNVGWPR DGTFNRDLIT QVKIKVFSPG PHGHPDQVPY IVTWEALAFD
PPPWAKPFVH PKPPPPLPPS APSLPLEPPL STPSRSSLYP ALTPSLGAKP KPQVLSDSEG
PLIDLLTEDP PPYRDPRPPP SDGDGNSGEA TPAGEAPDPS PMASRLRGRR EPPVADSTTS
QAFPLRTGGN GQLQYWPFSS SDLYNWKNNN PSFSEDPGKL TALIESVLIT HQPTWDDCQQ
LLGTLLTGEE KQRVLLEARK AVRGDDGRPT QLPNEVDAAF PLERPDWEYT TQAGRNHLVQ
YRQLLLAGLQ NAGRSPTNLA KVKGITQGPN ESPSAFLERL KEAYRRYTPY DPEDPGQETN
VAMSFIWQSA PDIGRKLERL EDLKNKTLGD LVREAERIFN KRETPEEREE RIRRETEEKE
ERRRTEDEQK EKERDRRRHR EMSKLLATVV SGQRQDRQGG ERRRSQLDRD QCAYCKEKGH
WAKDCPKKPR GPRGPRPQTS LLTLDD
