GGC1_YEAST
ID GGC1_YEAST Reviewed; 300 AA.
AC P38988; D6VRF6;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Mitochondrial GTP/GDP carrier protein 1;
GN Name=GGC1; Synonyms=SHM1, YHM1; OrderedLocusNames=YDL198C; ORFNames=D1214;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8905928;
RX DOI=10.1002/(sici)1097-0061(19960930)12:12<1239::aid-yea17>3.0.co;2-8;
RA Kao L.-R., Megraw T.L., Chae C.-B.;
RT "SHM1: a multicopy suppressor of a temperature-sensitive null mutation in
RT the HMG1-like abf2 gene.";
RL Yeast 12:1239-1250(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8896272;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b<1065::aid-yea995>3.0.co;2-f;
RA Verhasselt P., Voet M., Mathys J., Volckaert G.;
RT "The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast
RT chromosome IV reveals the location of five known genes and characterizes at
RT least six new open reading frames including putative genes for ribosomal
RT protein L35 and a sugar transport protein.";
RL Yeast 12:1065-1070(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN [7]
RP FUNCTION.
RX PubMed=14629196; DOI=10.1042/bj20031387;
RA Lesuisse E., Lyver E.R., Knight S.A., Dancis A.;
RT "Role of YHM1, encoding a mitochondrial carrier protein, in iron
RT distribution of yeast.";
RL Biochem. J. 378:599-607(2004).
RN [8]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=14998997; DOI=10.1074/jbc.m313610200;
RA Vozza A., Blanco E., Palmieri L., Palmieri F.;
RT "Identification of the mitochondrial GTP/GDP transporter in Saccharomyces
RT cerevisiae.";
RL J. Biol. Chem. 279:20850-20857(2004).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Mitochondrial GTP/GDP transporter required for GTP uptake and
CC GDP exit from mitochondria. Involved in mitochondrial iron transport
CC and essential for mitochondrial genome maintenance.
CC {ECO:0000269|PubMed:14629196, ECO:0000269|PubMed:14998997}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.2 uM for GTP {ECO:0000269|PubMed:14998997};
CC KM=4.7 uM for GDP {ECO:0000269|PubMed:14998997};
CC KM=15.9 uM for dGTP {ECO:0000269|PubMed:14998997};
CC Vmax=2.0 mmol/min/g enzyme {ECO:0000269|PubMed:14998997};
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:14576278}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14576278}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; U08352; AAC49493.1; -; Genomic_DNA.
DR EMBL; X83276; CAA58249.1; -; Genomic_DNA.
DR EMBL; X99000; CAA67471.1; -; Genomic_DNA.
DR EMBL; Z74246; CAA98775.1; -; Genomic_DNA.
DR EMBL; AY693086; AAT93105.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11666.1; -; Genomic_DNA.
DR PIR; S58779; S58779.
DR RefSeq; NP_010083.1; NM_001180258.1.
DR AlphaFoldDB; P38988; -.
DR SASBDB; P38988; -.
DR SMR; P38988; -.
DR BioGRID; 31848; 36.
DR DIP; DIP-7756N; -.
DR IntAct; P38988; 21.
DR MINT; P38988; -.
DR STRING; 4932.YDL198C; -.
DR TCDB; 2.A.29.21.1; the mitochondrial carrier (mc) family.
DR MaxQB; P38988; -.
DR PaxDb; P38988; -.
DR PRIDE; P38988; -.
DR EnsemblFungi; YDL198C_mRNA; YDL198C; YDL198C.
DR GeneID; 851329; -.
DR KEGG; sce:YDL198C; -.
DR SGD; S000002357; GGC1.
DR VEuPathDB; FungiDB:YDL198C; -.
DR eggNOG; KOG0750; Eukaryota.
DR HOGENOM; CLU_053371_0_0_1; -.
DR InParanoid; P38988; -.
DR OMA; KATFFQN; -.
DR BioCyc; YEAST:G3O-29582-MON; -.
DR PRO; PR:P38988; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P38988; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0001409; F:guanine nucleotide transmembrane transporter activity; IDA:SGD.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IMP:SGD.
DR GO; GO:0001408; P:guanine nucleotide transport; IDA:SGD.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IDA:SGD.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 2.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..300
FT /note="Mitochondrial GTP/GDP carrier protein 1"
FT /id="PRO_0000090687"
FT TRANSMEM 14..34
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..101
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..189
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..285
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 8..108
FT /note="Solcar 1"
FT REPEAT 117..198
FT /note="Solcar 2"
FT REPEAT 208..293
FT /note="Solcar 3"
SQ SEQUENCE 300 AA; 33215 MW; 3B0AB95EF563E85C CRC64;
MPHTDKKQSG LARLLGSASA GIMEIAVFHP VDTISKRLMS NHTKITSGQE LNRVIFRDHF
SEPLGKRLFT LFPGLGYAAS YKVLQRVYKY GGQPFANEFL NKHYKKDFDN LFGEKTGKAM
RSAAAGSLIG IGEIVLLPLD VLKIKRQTNP ESFKGRGFIK ILRDEGLFNL YRGWGWTAAR
NAPGSFALFG GNAFAKEYIL GLKDYSQATW SQNFISSIVG ACSSLIVSAP LDVIKTRIQN
RNFDNPESGL RIVKNTLKNE GVTAFFKGLT PKLLTTGPKL VFSFALAQSL IPRFDNLLSK
