ALR2_YEAST
ID ALR2_YEAST Reviewed; 858 AA.
AC P43553; D6VTI0;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Magnesium transporter ALR2;
DE AltName: Full=Aluminum resistance protein 2;
GN Name=ALR2; OrderedLocusNames=YFL050C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=9430719; DOI=10.1074/jbc.273.3.1727;
RA MacDiarmid C.W., Gardner R.C.;
RT "Overexpression of the Saccharomyces cerevisiae magnesium transport system
RT confers resistance to aluminum ion.";
RL J. Biol. Chem. 273:1727-1732(1998).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Plasma membrane magnesium transporter.
CC {ECO:0000269|PubMed:9430719}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:9430719};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 2130 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35)
CC family. {ECO:0000305}.
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DR EMBL; D50617; BAA09191.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12390.1; -; Genomic_DNA.
DR PIR; S56205; S56205.
DR RefSeq; NP_116604.1; NM_001179917.1.
DR AlphaFoldDB; P43553; -.
DR SMR; P43553; -.
DR BioGRID; 31097; 39.
DR DIP; DIP-4581N; -.
DR STRING; 4932.YFL050C; -.
DR iPTMnet; P43553; -.
DR MaxQB; P43553; -.
DR PaxDb; P43553; -.
DR PRIDE; P43553; -.
DR EnsemblFungi; YFL050C_mRNA; YFL050C; YFL050C.
DR GeneID; 850494; -.
DR KEGG; sce:YFL050C; -.
DR SGD; S000001844; ALR2.
DR VEuPathDB; FungiDB:YFL050C; -.
DR eggNOG; ENOG502QPTQ; Eukaryota.
DR GeneTree; ENSGT00940000176664; -.
DR HOGENOM; CLU_007127_6_0_1; -.
DR InParanoid; P43553; -.
DR OMA; VCFPMVP; -.
DR BioCyc; YEAST:G3O-30415-MON; -.
DR BRENDA; 7.2.2.14; 984.
DR PRO; PR:P43553; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P43553; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IMP:SGD.
DR GO; GO:0022890; F:inorganic cation transmembrane transporter activity; IMP:SGD.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006812; P:cation transport; IMP:SGD.
DR GO; GO:0010961; P:cellular magnesium ion homeostasis; IBA:GO_Central.
DR GO; GO:0015693; P:magnesium ion transport; IDA:SGD.
DR GO; GO:0055085; P:transmembrane transport; IMP:SGD.
DR CDD; cd12829; Alr1p-like; 1.
DR InterPro; IPR044089; Alr1-like.
DR InterPro; IPR045861; CorA_cytoplasmic_dom.
DR InterPro; IPR045863; CorA_TM1_TM2.
DR InterPro; IPR002523; MgTranspt_CorA/ZnTranspt_ZntB.
DR Pfam; PF01544; CorA; 1.
DR SUPFAM; SSF143865; SSF143865; 1.
DR SUPFAM; SSF144083; SSF144083; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Magnesium; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..858
FT /note="Magnesium transporter ALR2"
FT /id="PRO_0000201536"
FT TOPO_DOM 1..741
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 742..762
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 763..771
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 772..792
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 793..858
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 318..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 365..396
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..67
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 322..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 858 AA; 96684 MW; 7AAFEC6086FF0618 CRC64;
MSSLSTSFDS SSDLPRSKSV DNTAASMKTG KYPKLENYRQ YSDAQPIRHE ALALKVDETK
DSRHKFSSSN GENSGVENGG YVEKTNISTS GRMDFEGEAE AEAVKRYQLR SFALLSSNAR
PSRLAKSETH QKQIHVESIA PSLPKNAALE RGHDTALPAG TSSNRCNLEA SSSARTFTSA
RKASLVSAIF ETSAESEHGT HPKQAKLKRR TYSTISTHSS VNPTTLLTRT ASQKSDMGND
TRRIKPLRMD SRVSFHSEIS QASRDSQETE EDVCFPMFRL LHTRVNGVDF DELEEYAQIS
NAERNLSLAN HQRHSERTYN HTDQDTGFTN SASTSGSSAA LKYTPEISRT LEKNCSVNEM
YVSENNESVR EDDKPDLHPD VTFGRNKIEG EKEGNDSSYS RAYYTLQNTE YQIPSRFSFF
RSESDETVHA SDIPSLISEG QTFYELFKGG DPTWWLDCSC PTDDEMRCIA KTFGIHPLTA
EDIRMQETRE KVELFKSYYF VCFHTFENDK ESENYLEPIN VYIVVFRSGV LTFHFDPISH
CANVRRRVRQ LRDYVSVNSD WLCYALIDDI TDSFAPVIQS IEYEADSIDD SVFMTRDMDF
AAMLQRIGES RRKTMTLMRL LSGKADVIKM FAKRCQDETN GIGPVLKSQT NMVNLQAEQE
NVNQNNSNNQ ISLSNSYMQT TSQPRGDIAL YLGDIQDHLL TMFQNLLAYE KIFSRSHANY
LAQLQVESFN SNNKVTEMLG KVTMLGTMLV PLNVITGLFG MNVKVPGRNG SIAWWYGILG
VLLLLAVISW FLASYWIKKI DPPATLNEAA GSGAKSVISS FLPKRDKRFN DDSKNGNARV
GVRRKSTVSL PSRYSRYN
