ID   ALRB_DICDI              Reviewed;         311 AA.
AC   Q54NZ7;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 2.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Aldose reductase B;
DE            Short=ARB;
DE            EC=1.1.1.21;
DE   AltName: Full=Aldehyde reductase B;
GN   Name=alrB; ORFNames=DDB_G0285053;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Catalyzes the NADPH-dependent reduction of a wide variety of
CC       carbonyl-containing compounds to their corresponding alcohols with a
CC       broad range of catalytic efficiencies. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alditol + NAD(+) = an aldose + H(+) + NADH;
CC         Xref=Rhea:RHEA:12785, Rhea:RHEA-COMP:9554, Rhea:RHEA-COMP:9555,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15693, ChEBI:CHEBI:17522,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.21;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alditol + NADP(+) = an aldose + H(+) + NADPH;
CC         Xref=Rhea:RHEA:12789, Rhea:RHEA-COMP:9554, Rhea:RHEA-COMP:9555,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15693, ChEBI:CHEBI:17522,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.21;
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR   EMBL; AAFI02000073; EAL64990.2; -; Genomic_DNA.
DR   RefSeq; XP_639920.2; XM_634828.2.
DR   AlphaFoldDB; Q54NZ7; -.
DR   SMR; Q54NZ7; -.
DR   STRING; 44689.DDB0231282; -.
DR   PaxDb; Q54NZ7; -.
DR   EnsemblProtists; EAL64990; EAL64990; DDB_G0285053.
DR   GeneID; 8624832; -.
DR   KEGG; ddi:DDB_G0285053; -.
DR   dictyBase; DDB_G0285053; alrB.
DR   eggNOG; KOG1577; Eukaryota.
DR   HOGENOM; CLU_023205_0_3_1; -.
DR   InParanoid; Q54NZ7; -.
DR   OMA; WCIDQDI; -.
DR   PhylomeDB; Q54NZ7; -.
DR   PRO; PR:Q54NZ7; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004032; F:alditol:NADP+ 1-oxidoreductase activity; IBA:GO_Central.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   3: Inferred from homology;
KW   NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..311
FT                   /note="Aldose reductase B"
FT                   /id="PRO_0000327646"
FT   ACT_SITE        54
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         13..23
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000255"
FT   BINDING         116
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         219..273
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   311 AA;  35060 MW;  F60EBB244639962D CRC64;
     MSQPQNTFKL NDDIHHIPMI GLGTYNGAKV GEVGDAVKVA LKSGYRHIDG AAIYMNEKEI
     GHALKEVFAE GEIKREDIFY VSKLWNSCHH ASLVRKHCEK TLEDLGLEYL DLYLIHWPIA
     FENADPSGTT TQPLRDSDGE PVLAAVSIRE TWQEMEKLVE YGLVKSIGVS NFNVQNLVDL
     LTYAKIKPAI NQVEVHPYLS QPNLKYFCDR YGIVLTAYSP LGQGKCDLLS NETLKSIADK
     HNKTVANVIF KWLNQRGIVT IPKSSNPARI IENFNIFDFQ LSNEDMDKIN SLNSNLRTCT
     PANFCKIPLF D