GGN1_LIMKU
ID GGN1_LIMKU Reviewed; 70 AA.
AC C3RT17;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 1.
DT 25-MAY-2022, entry version 28.
DE RecName: Full=Gaegurin-LK1 {ECO:0000303|PubMed:23054029};
DE Flags: Precursor;
OS Limnonectes kuhlii (Kuhl's Creek frog) (Rana kuhlii).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Ranoidea; Dicroglossidae; Dicroglossinae;
OC Limnonectes.
OX NCBI_TaxID=110107;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 47-70, FUNCTION,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC TISSUE=Skin {ECO:0000303|PubMed:23054029}, and
RC Skin secretion {ECO:0000303|PubMed:23054029};
RX PubMed=23054029; DOI=10.1007/s11033-012-2152-4;
RA Wang G., Wang Y., Ma D., Liu H., Li J., Zhang K., Yang X., Lai R., Liu J.;
RT "Five novel antimicrobial peptides from the Kuhl's wart frog skin
RT secretions, Limnonectes kuhlii.";
RL Mol. Biol. Rep. 40:1097-1102(2013).
CC -!- FUNCTION: Has antimicrobial activity against Gram-positive bacteria
CC S.aureus ATCC 2592 (MIC=5.0 uM), S.aureus ATCC 43300 (MIC=10.0 uM) and
CC B.subtilis (MIC=20.0 uM), against Gram-negative bacteria E.coli ML-35P
CC (MIC=50.0 uM), P.aeruginosa PA01 (MIC=10.0 uM) and P.aeruginosa ATCC
CC 27853 (MIC=5.0 uM) and against fungus C.albicans ATCC 2002 (MIC=10.0
CC uM). {ECO:0000269|PubMed:23054029}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255,
CC ECO:0000269|PubMed:23054029}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000269|PubMed:23054029}.
CC -!- MASS SPECTROMETRY: Mass=2565.1; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:23054029};
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Brevinin subfamily. {ECO:0000305}.
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DR EMBL; EU346900; ACB12316.1; -; mRNA.
DR AlphaFoldDB; C3RT17; -.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR GO; GO:0031640; P:killing of cells of another organism; IDA:UniProtKB.
DR InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR Pfam; PF03032; FSAP_sig_propep; 1.
PE 1: Evidence at protein level;
KW Amphibian defense peptide; Antibiotic; Antimicrobial;
KW Cleavage on pair of basic residues; Direct protein sequencing; Fungicide;
KW Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..44
FT /evidence="ECO:0000305|PubMed:23054029"
FT /id="PRO_0000445399"
FT PEPTIDE 47..70
FT /note="Gaegurin-LK1"
FT /evidence="ECO:0000269|PubMed:23054029"
FT /id="PRO_0000445400"
SQ SEQUENCE 70 AA; 8035 MW; DCE5E48C857A9912 CRC64;
MFTMKKSLLF LFFLGTINLS LCEEERNAEE EKRDGDDEMD VEVQKRFIGP VLKMATSILP
TAICKGFKKC