GGNB2_MOUSE
ID GGNB2_MOUSE Reviewed; 696 AA.
AC Q5SV77; A4QPD5; B7ZP33; Q5SV75; Q5SV76; Q5SV78; Q6GVH6; Q6P9J4; Q920N4;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Gametogenetin-binding protein 2;
DE AltName: Full=Dioxin-inducible factor 3;
DE Short=DIF-3;
DE AltName: Full=Protein ZNF403;
GN Name=Ggnbp2; Synonyms=Zfp403, Znf403;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=11728448; DOI=10.1016/s0014-5793(01)03039-3;
RA Ohbayashi T., Oikawa K., Iwata R., Kameta A., Evine K., Isobe T.,
RA Matsuda Y., Mimura J., Fujii-Kuriyama Y., Kuroda M., Mukai K.;
RT "Dioxin induces a novel nuclear factor, DIF-3, that is implicated in
RT spermatogenesis.";
RL FEBS Lett. 508:341-344(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, AND
RP INTERACTION WITH GGN.
RX PubMed=15642376; DOI=10.1016/j.febslet.2004.10.112;
RA Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B.;
RT "Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential
RT interaction partners of testicular germ cell-specific protein GGN1.";
RL FEBS Lett. 579:559-566(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC STRAIN=NMRI; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-602, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be involved in spermatogenesis.
CC -!- SUBUNIT: Interacts with isoform 1 of GGN.
CC {ECO:0000269|PubMed:15642376}.
CC -!- INTERACTION:
CC Q5SV77; Q80WJ1: Ggn; NbExp=3; IntAct=EBI-4370069, EBI-3890505;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC {ECO:0000269|PubMed:15642376}. Note=Associated with vesicular
CC structures.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q5SV77-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5SV77-2; Sequence=VSP_019180;
CC Name=3;
CC IsoId=Q5SV77-3; Sequence=VSP_019179, VSP_019180;
CC Name=4;
CC IsoId=Q5SV77-4; Sequence=VSP_019179;
CC -!- TISSUE SPECIFICITY: Testis-specific. {ECO:0000269|PubMed:11728448}.
CC -!- DEVELOPMENTAL STAGE: Abundantly expressed during spermatogenesis. Not
CC detected in newborn animals, in which spermatogenesis has not yet
CC progressed beyond the earliest stages.
CC -!- INDUCTION: By dioxin (2,3,7,8-Tetrachlorodibenzo-p-dioxin).
CC {ECO:0000269|PubMed:11728448}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH60738.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
CC Sequence=CAI24983.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAI25484.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAI25487.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB064543; BAB71783.1; -; mRNA.
DR EMBL; AY633741; AAT47120.1; -; mRNA.
DR EMBL; AL596083; CAI24981.1; -; Genomic_DNA.
DR EMBL; AL645623; CAI24981.1; JOINED; Genomic_DNA.
DR EMBL; AL596083; CAI24982.1; -; Genomic_DNA.
DR EMBL; AL645623; CAI24982.1; JOINED; Genomic_DNA.
DR EMBL; AL596083; CAI24983.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL645623; CAI24983.1; JOINED; Genomic_DNA.
DR EMBL; AL645623; CAI25484.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL645623; CAI25485.1; -; Genomic_DNA.
DR EMBL; AL596083; CAI25485.1; JOINED; Genomic_DNA.
DR EMBL; AL645623; CAI25486.1; -; Genomic_DNA.
DR EMBL; AL596083; CAI25486.1; JOINED; Genomic_DNA.
DR EMBL; AL645623; CAI25487.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL596083; CAI25487.1; JOINED; Genomic_DNA.
DR EMBL; BC060738; AAH60738.1; ALT_SEQ; mRNA.
DR EMBL; BC139772; AAI39773.1; -; mRNA.
DR EMBL; BC145605; AAI45606.1; -; mRNA.
DR EMBL; BC150801; AAI50802.1; -; mRNA.
DR CCDS; CCDS36262.1; -. [Q5SV77-2]
DR CCDS; CCDS88210.1; -. [Q5SV77-1]
DR CCDS; CCDS88211.1; -. [Q5SV77-4]
DR RefSeq; NP_694784.2; NM_153144.2. [Q5SV77-2]
DR RefSeq; XP_011247235.1; XM_011248933.2. [Q5SV77-1]
DR RefSeq; XP_017169962.1; XM_017314473.1. [Q5SV77-2]
DR RefSeq; XP_017169963.1; XM_017314474.1.
DR RefSeq; XP_017169964.1; XM_017314475.1.
DR AlphaFoldDB; Q5SV77; -.
DR SMR; Q5SV77; -.
DR BioGRID; 229837; 1.
DR IntAct; Q5SV77; 3.
DR MINT; Q5SV77; -.
DR STRING; 10090.ENSMUSP00000018547; -.
DR iPTMnet; Q5SV77; -.
DR PhosphoSitePlus; Q5SV77; -.
DR EPD; Q5SV77; -.
DR MaxQB; Q5SV77; -.
DR PaxDb; Q5SV77; -.
DR PRIDE; Q5SV77; -.
DR ProteomicsDB; 267436; -. [Q5SV77-1]
DR ProteomicsDB; 267437; -. [Q5SV77-2]
DR ProteomicsDB; 267438; -. [Q5SV77-3]
DR ProteomicsDB; 267439; -. [Q5SV77-4]
DR Antibodypedia; 73502; 95 antibodies from 17 providers.
DR DNASU; 217039; -.
DR Ensembl; ENSMUST00000018547; ENSMUSP00000018547; ENSMUSG00000020530. [Q5SV77-4]
DR Ensembl; ENSMUST00000108081; ENSMUSP00000103716; ENSMUSG00000020530. [Q5SV77-2]
DR Ensembl; ENSMUST00000154915; ENSMUSP00000117482; ENSMUSG00000020530. [Q5SV77-1]
DR Ensembl; ENSMUST00000168434; ENSMUSP00000130013; ENSMUSG00000020530. [Q5SV77-1]
DR Ensembl; ENSMUST00000172405; ENSMUSP00000127584; ENSMUSG00000020530. [Q5SV77-2]
DR GeneID; 217039; -.
DR KEGG; mmu:217039; -.
DR UCSC; uc007kqu.1; mouse. [Q5SV77-1]
DR UCSC; uc007kqv.1; mouse. [Q5SV77-2]
DR UCSC; uc011ybk.1; mouse. [Q5SV77-4]
DR CTD; 79893; -.
DR MGI; MGI:2387356; Ggnbp2.
DR VEuPathDB; HostDB:ENSMUSG00000020530; -.
DR eggNOG; ENOG502QQ20; Eukaryota.
DR GeneTree; ENSGT00390000009552; -.
DR InParanoid; Q5SV77; -.
DR OMA; SEEKGYC; -.
DR OrthoDB; 398180at2759; -.
DR PhylomeDB; Q5SV77; -.
DR TreeFam; TF323487; -.
DR BioGRID-ORCS; 217039; 11 hits in 70 CRISPR screens.
DR ChiTaRS; Ggnbp2; mouse.
DR PRO; PR:Q5SV77; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5SV77; protein.
DR Bgee; ENSMUSG00000020530; Expressed in spermatid and 254 other tissues.
DR ExpressionAtlas; Q5SV77; baseline and differential.
DR Genevisible; Q5SV77; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0030154; P:cell differentiation; IDA:MGI.
DR GO; GO:0060716; P:labyrinthine layer blood vessel development; IMP:MGI.
DR GO; GO:0060711; P:labyrinthine layer development; IMP:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IDA:MGI.
DR GO; GO:0010629; P:negative regulation of gene expression; IDA:MGI.
DR GO; GO:0033140; P:negative regulation of peptidyl-serine phosphorylation of STAT protein; IMP:MGI.
DR GO; GO:0061099; P:negative regulation of protein tyrosine kinase activity; IMP:MGI.
DR GO; GO:0042532; P:negative regulation of tyrosine phosphorylation of STAT protein; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR026073; GGNBP2.
DR PANTHER; PTHR13601; PTHR13601; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Developmental protein;
KW Differentiation; Phosphoprotein; Reference proteome; Spermatogenesis.
FT CHAIN 1..696
FT /note="Gametogenetin-binding protein 2"
FT /id="PRO_0000239349"
FT MOD_RES 360
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H3C7"
FT MOD_RES 602
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 176
FT /note="G -> GEGSSSSVSSEKLSTDKRSSEDHRKDSKCRIIFHYGPFQGTAR (in
FT isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:11728448,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_019179"
FT VAR_SEQ 282
FT /note="G -> GYE (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:11728448,
FT ECO:0000303|PubMed:15642376"
FT /id="VSP_019180"
FT CONFLICT 510
FT /note="D -> N (in Ref. 4; AAI45606/AAI50802)"
FT /evidence="ECO:0000305"
FT CONFLICT Q5SV77-3:219
FT /note="G -> GG (in Ref. 1; BAB71783)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 696 AA; 78970 MW; C4F3976169B8646C CRC64;
MARLVAVCRD GEEEFPFERR QIPLYIDDTL TMVMEFPDNV LNLDGHQNNG AQLKQFIQRH
SMLKQQDLSI AMVVTSREVL SALSQLVPCV GCRRSVERLF SQLVESGNPA LEPLTVGPKG
VLSLTRSCMT DAKKLYTLFY VHGSKLNDMI DAIPKSKKNK RCQLHSLDTH KPKPLGGCWM
DVWELMSQEC RDEVVLIDSS CLLETLETYL RKHRFCTDCK NKVLRAYNIL IGELDCSKEK
GYCAALYEGL RCCPHERHIH VCCETDFIAH LLGRAEPEFA GGRRERHAKT IDIAQEEVLT
CLGIHLYERL HRIWQKLRAE EQTWQMLFYL GVDALRKSFE MTVEKVQGIS RLEQLCEEFS
EEERVRELKQ EKKRQKRKNR RKNKCVCDTP ASLHTADEKA VSREKETDFI ENSCNACGSA
EDGETCVEVM VTSENTSCTC PSSGNLLGSP KIKKGMSPHC NGSDCGYSSS MEGSETGSRE
GSDVACTEGI CNHDEHGEDS CVHHCEDKED DGDSCVECWA NSEENNIKGK NKKKKKKSKM
LKCDEHIQKL GSCITDPGNR ETSGNTMHTV FHRDKTKDAH PESCCSTEKG GQPLPWFEHR
KSVPQFTEPT EMSFGPDSGK GAKSLVELLD ESECTSDEEI FISQDEIQSF MANNQSFYSN
REQYRQHLKE KFNKYCRLND HKRPVCSGWL TTAGAN
