GGN_MOUSE
ID GGN_MOUSE Reviewed; 673 AA.
AC Q80WJ1; Q5EBP4; Q80WI9; Q80WJ0;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Gametogenetin;
GN Name=Ggn;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INTERACTION WITH
RP FANCL.
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=12574169; DOI=10.1074/jbc.m211023200;
RA Lu B., Bishop C.E.;
RT "Mouse GGN1 and GGN3, two germ cell-specific proteins from the single gene
RT Ggn, interact with mouse POG and play a role in spermatogenesis.";
RL J. Biol. Chem. 278:16289-16296(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH GGNBP1; ZNF403 AND OAZ3.
RX PubMed=15642376; DOI=10.1016/j.febslet.2004.10.112;
RA Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B.;
RT "Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential
RT interaction partners of testicular germ cell-specific protein GGN1.";
RL FEBS Lett. 579:559-566(2005).
RN [4]
RP INTERACTION WITH GGNBP1, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=15892049; DOI=10.1002/mrd.20313;
RA Zhao Q., Zhou Y., Cao Z., Zhu H., Huang P., Lu B.;
RT "Germ-cell specific protein gametogenetin protein 2 (GGN2), expression in
RT the testis, and association with intracellular membrane.";
RL Mol. Reprod. Dev. 72:31-39(2005).
CC -!- FUNCTION: May be involved in spermatogenesis.
CC {ECO:0000269|PubMed:12574169}.
CC -!- SUBUNIT: Isoform 1 and isoform 3 interact with FANCL. Isoform 1
CC interacts with GGNBP1, ZNF403/GGNBP2 and OAZ3. Isoform 2 interacts with
CC GGNBP1. {ECO:0000269|PubMed:12574169, ECO:0000269|PubMed:15642376,
CC ECO:0000269|PubMed:15892049}.
CC -!- INTERACTION:
CC Q80WJ1; Q5SV77: Ggnbp2; NbExp=3; IntAct=EBI-3890505, EBI-4370069;
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm, perinuclear region.
CC Note=Localizes along the nuclear membrane.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasmic vesicle.
CC -!- SUBCELLULAR LOCATION: [Isoform 3]: Nucleus, nucleolus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=GGN1;
CC IsoId=Q80WJ1-1; Sequence=Displayed;
CC Name=2; Synonyms=GGN2;
CC IsoId=Q80WJ1-2; Sequence=VSP_019172, VSP_019173;
CC Name=3; Synonyms=GGN3;
CC IsoId=Q80WJ1-3; Sequence=VSP_019171;
CC -!- TISSUE SPECIFICITY: Testis-specific. Specifically expressed in the germ
CC cells and not in the somatic, Sertoli, or Leydig cells. In adult
CC testis, expression starts in stage VIII pachytene spermatocytes,
CC increases in stage IX and X pachytene spermatocytes, and culminates in
CC stage XI diplotene spermatocytes and the meiotic cells in stage XII.
CC Expression decreases slightly in step 1-3 spermatids, further decreases
CC in step 4-11 spermatids, and is no longer detectable in step 12
CC spermatids and beyond. Isoform 2 is mainly expressed in testis.
CC {ECO:0000269|PubMed:12574169}.
CC -!- DEVELOPMENTAL STAGE: Isoform 2 is detected at high level in adult
CC testis and at lower level in 19-day testis. Not detected in 14-day
CC testis (at protein level). In the developing postnatal gonad, it is not
CC expressed in 6-day-old testes in which the germ cells are almost
CC exclusively spermatogonia or 14-day testis in which the most advanced
CC germ cells are early pachytene spermatocytes. However, it is expressed
CC in 21-day testis tubules containing late pachytene spermatocytes or
CC spermatids. In the postnatal male testis, it is strictly confined to
CC late pachytene spermatocytes through spermatids, a time during which
CC meiosis takes place. {ECO:0000269|PubMed:12574169,
CC ECO:0000269|PubMed:15892049}.
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DR EMBL; AF538032; AAP31497.1; -; mRNA.
DR EMBL; AF538033; AAP31498.1; -; mRNA.
DR EMBL; AF538034; AAP31499.1; -; mRNA.
DR EMBL; BC089358; AAH89358.1; -; mRNA.
DR CCDS; CCDS21067.1; -. [Q80WJ1-3]
DR CCDS; CCDS90196.1; -. [Q80WJ1-1]
DR RefSeq; NP_874353.2; NM_182694.2.
DR RefSeq; NP_874355.1; NM_182696.2. [Q80WJ1-3]
DR RefSeq; XP_006540008.1; XM_006539945.3.
DR AlphaFoldDB; Q80WJ1; -.
DR BioGRID; 232577; 5.
DR IntAct; Q80WJ1; 3.
DR MINT; Q80WJ1; -.
DR STRING; 10090.ENSMUSP00000096209; -.
DR PhosphoSitePlus; Q80WJ1; -.
DR PaxDb; Q80WJ1; -.
DR PRIDE; Q80WJ1; -.
DR ProteomicsDB; 267440; -. [Q80WJ1-1]
DR ProteomicsDB; 267441; -. [Q80WJ1-2]
DR ProteomicsDB; 267442; -. [Q80WJ1-3]
DR Antibodypedia; 30056; 69 antibodies from 17 providers.
DR Ensembl; ENSMUST00000033886; ENSMUSP00000033886; ENSMUSG00000031493. [Q80WJ1-3]
DR Ensembl; ENSMUST00000209019; ENSMUSP00000146750; ENSMUSG00000031493. [Q80WJ1-1]
DR GeneID; 243897; -.
DR KEGG; mmu:243897; -.
DR UCSC; uc009gaz.1; mouse. [Q80WJ1-3]
DR CTD; 199720; -.
DR MGI; MGI:2181461; Ggn.
DR VEuPathDB; HostDB:ENSMUSG00000031493; -.
DR eggNOG; ENOG502ST22; Eukaryota.
DR GeneTree; ENSGT00700000104635; -.
DR HOGENOM; CLU_1864469_0_0_1; -.
DR InParanoid; Q80WJ1; -.
DR OMA; PPGQMHS; -.
DR OrthoDB; 1139345at2759; -.
DR PhylomeDB; Q80WJ1; -.
DR BioGRID-ORCS; 243897; 2 hits in 107 CRISPR screens.
DR PRO; PR:Q80WJ1; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q80WJ1; protein.
DR Bgee; ENSMUSG00000031493; Expressed in testis and 55 other tissues.
DR ExpressionAtlas; Q80WJ1; baseline and differential.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005635; C:nuclear envelope; NAS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
DR GO; GO:0042802; F:identical protein binding; IPI:MGI.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IPI:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006302; P:double-strand break repair; IMP:MGI.
DR GO; GO:0007566; P:embryo implantation; IMP:MGI.
DR GO; GO:0007276; P:gamete generation; IEP:UniProtKB.
DR GO; GO:0008104; P:protein localization; IDA:MGI.
DR GO; GO:0065003; P:protein-containing complex assembly; NAS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR031400; GGN.
DR PANTHER; PTHR47742; PTHR47742; 1.
DR Pfam; PF15685; GGN; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoplasmic vesicle;
KW Developmental protein; Differentiation; Nucleus; Phosphoprotein;
KW Reference proteome; Spermatogenesis.
FT CHAIN 1..673
FT /note="Gametogenetin"
FT /id="PRO_0000239343"
FT REGION 1..599
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 125..506
FT /note="Interaction with GGNBP1"
FT REGION 511..673
FT /note="Interactions with ZNF403/GGNBP2 and OAZ3"
FT COMPBIAS 80..107
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 158..182
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 184..209
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..355
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 434..485
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 498..541
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 572..598
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 399
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66HC8"
FT VAR_SEQ 1..536
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12574169"
FT /id="VSP_019171"
FT VAR_SEQ 204..269
FT /note="VRHSSEGQAAGGAHGGVPPQAGEGEMARSATSESGLSLLCKVTFKSGPHLSP
FT TSASGPLAAKASPG -> LPTRSQLQLQPQSHPKFQQPPPLSCHRQCPNPRLVHAGTKV
FT PERPGVLSVKKGLLETALENRIRLR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12574169"
FT /id="VSP_019172"
FT VAR_SEQ 270..673
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12574169"
FT /id="VSP_019173"
FT CONFLICT 87
FT /note="P -> S (in Ref. 1; AAP31497/AAP31498)"
FT /evidence="ECO:0000305"
FT CONFLICT 91
FT /note="E -> EAA (in Ref. 1; AAP31497/AAP31498)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 673 AA; 68892 MW; 0D48C73F84650705 CRC64;
MGNVQSEPSA GGGSRKEQAS DRASDSRRTP LVEPEVTPSS PAMRLARGLG VWFPGSSGPP
GLLIPPEPQA SSSPLPLTLE LPSPVTPPPE EAAAVSTPPP PPVGTLLPAP SKWRKPTGTS
VPRIRGLLEA SHRGQGDPPS LRPLPPLPRQ LTEKDPVLRA PAPPPTPLEP RKQLPPAPST
CDPQPLSRRI TLASSATSPT ESQVRHSSEG QAAGGAHGGV PPQAGEGEMA RSATSESGLS
LLCKVTFKSG PHLSPTSASG PLAAKASPGA GGGGLFASSG AISYAEVLKQ GPQPPGATRP
LGEVPPGATR PLGEVPRAAQ ETEGGDGDGE GCSGPPSVPA PLARALPPPP YTTFPGSKPK
FDWVSPPDGT ERHFRFNGAV GGIGAPRRRT TTLSGPWGSP PPRSGQTHPS SGPRRPTPAL
LAPPMFIFPA PNNGEPVRPV PPSPQQIPPL PPPPPTPPAT PPPAPPPTPQ PPALPRTPIL
VARPPTPGPG HLESALAPTP PSTLSPTAAA DQVPAATPAT VTSQVPATAT AELSPPMPQP
KTRTRRNKGP RAARGVIREE GTSGDGPREP NTAPVTDSSS GGGGGGSNGT STAGASNKGT
ARHWPPFEVL NSCPCKCYCR HQRRHRRLPR NVSAWLSTPT NHLSEPPWVA TVKLAGSLVA
GLEHYDLQAT HST
