GGYF2_DANRE
ID GGYF2_DANRE Reviewed; 1329 AA.
AC Q4KME6; B0S7J5;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=GRB10-interacting GYF protein 2;
DE AltName: Full=PERQ amino acid-rich with GYF domain-containing protein 2;
DE AltName: Full=Trinucleotide repeat-containing gene 15 protein;
GN Name=gigyf2; Synonyms=perq2, tnrc15; ORFNames=zgc:111944;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=20060621; DOI=10.1016/j.neurobiolaging.2009.12.016;
RA Guella I., Pistocchi A., Asselta R., Rimoldi V., Ghilardi A., Sironi F.,
RA Trotta L., Primignani P., Zini M., Zecchinelli A., Coviello D., Pezzoli G.,
RA Del Giacco L., Duga S., Goldwurm S.;
RT "Mutational screening and zebrafish functional analysis of GIGYF2 as a
RT Parkinson-disease gene.";
RL Neurobiol. Aging 32:1994-2005(2011).
CC -!- FUNCTION: Key component of the 4EHP-GYF2 complex, a multiprotein
CC complex that acts as a repressor of translation initiation.
CC {ECO:0000250|UniProtKB:Q6Y7W6, ECO:0000250|UniProtKB:Q6Y7W8}.
CC -!- SUBUNIT: Component of the 4EHP-GYF2 complex (By similarity).
CC {ECO:0000250|UniProtKB:Q6Y7W6, ECO:0000250|UniProtKB:Q6Y7W8}.
CC -!- DEVELOPMENTAL STAGE: Maternally expressed: detected from the 1-cell
CC stage, prior to the onset of zygotic expression. From blastula until
CC gastrula stages, strongly expressed throughout the blastoderm. At 28
CC hours post-fertilization (hpf), highly expressed in the central nervous
CC system (CNS), whereas a weaker expression is found in the somites. At
CC later stages of development (48 hpf), expression persists in well-
CC defined structures of the CNS, such as the diencephalon, the
CC mesencephalon, the cerebellum and the rhombencephalon, and remains
CC detectable in the somites. {ECO:0000269|PubMed:20060621}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown show mild, intermediate, and
CC severe phenotypes. Embryos with severe phenotype display a disrupted
CC body pattern, embryos with intermediate phenotype show defects in the
CC head and eye structures, while embryos with mild phenotype only show a
CC twist in the terminal part of the tail. Embryos do not show important
CC cell loss in diencephalic dopaminergic (DA) neuron clusters.
CC {ECO:0000269|PubMed:20060621}.
CC -!- SIMILARITY: Belongs to the GIGYF family. {ECO:0000305}.
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DR EMBL; BX677676; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX890560; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX890608; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC098603; AAH98603.1; -; mRNA.
DR RefSeq; NP_001025332.2; NM_001030161.2.
DR AlphaFoldDB; Q4KME6; -.
DR SMR; Q4KME6; -.
DR STRING; 7955.ENSDARP00000056276; -.
DR PaxDb; Q4KME6; -.
DR PRIDE; Q4KME6; -.
DR Ensembl; ENSDART00000056277; ENSDARP00000056276; ENSDARG00000009735.
DR GeneID; 561830; -.
DR KEGG; dre:561830; -.
DR CTD; 26058; -.
DR ZFIN; ZDB-GENE-050706-185; gigyf2.
DR eggNOG; KOG1862; Eukaryota.
DR GeneTree; ENSGT00940000156108; -.
DR InParanoid; Q4KME6; -.
DR OMA; AIEMTEW; -.
DR OrthoDB; 412069at2759; -.
DR PhylomeDB; Q4KME6; -.
DR TreeFam; TF325513; -.
DR PRO; PR:Q4KME6; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 2.
DR Bgee; ENSDARG00000009735; Expressed in cleaving embryo and 32 other tissues.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0043204; C:perikaryon; IBA:GO_Central.
DR GO; GO:1990635; C:proximal dendrite; IBA:GO_Central.
DR GO; GO:0031982; C:vesicle; IBA:GO_Central.
DR GO; GO:0048009; P:insulin-like growth factor receptor signaling pathway; IBA:GO_Central.
DR CDD; cd00072; GYF; 1.
DR Gene3D; 3.30.1490.40; -; 1.
DR InterPro; IPR003169; GYF.
DR InterPro; IPR035445; GYF-like_dom_sf.
DR Pfam; PF02213; GYF; 1.
DR SMART; SM00444; GYF; 1.
DR SUPFAM; SSF55277; SSF55277; 1.
DR PROSITE; PS50829; GYF; 1.
PE 2: Evidence at transcript level;
KW Reference proteome.
FT CHAIN 1..1329
FT /note="GRB10-interacting GYF protein 2"
FT /id="PRO_0000270839"
FT DOMAIN 556..604
FT /note="GYF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00101"
FT REGION 109..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..419
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 720..740
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 781..1081
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1122..1155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1236..1256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1279..1302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..177
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..288
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..374
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 403..419
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 781..898
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 909..924
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 925..980
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 982..1002
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1003..1018
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1067..1081
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1127..1150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1241..1256
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 159
FT /note="S -> N (in Ref. 2; AAH98603)"
FT /evidence="ECO:0000305"
FT CONFLICT 363
FT /note="H -> Q (in Ref. 2; AAH98603)"
FT /evidence="ECO:0000305"
FT CONFLICT 388
FT /note="V -> D (in Ref. 2; AAH98603)"
FT /evidence="ECO:0000305"
FT CONFLICT 597
FT /note="D -> V (in Ref. 2; AAH98603)"
FT /evidence="ECO:0000305"
FT CONFLICT 914
FT /note="K -> E (in Ref. 2; AAH98603)"
FT /evidence="ECO:0000305"
FT CONFLICT 1048
FT /note="D -> DQQQQQQQ (in Ref. 2; AAH98603)"
FT /evidence="ECO:0000305"
FT CONFLICT 1071
FT /note="N -> I (in Ref. 2; AAH98603)"
FT /evidence="ECO:0000305"
FT CONFLICT 1275..1277
FT /note="QQL -> LQ (in Ref. 2; AAH98603)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1329 AA; 152268 MW; B974D4BEB2E8992F CRC64;
MAETQTLNFG PEWLRALAGG VGSSIVASPP LSPALPKYKL ADYRYGREEM LALYVKDNMI
PVDLHDKEFL PILQEEPLPP LALVPFTEEE QRNFSMSVNS AAVLRLTGRG GGTVAGAPRG
RSSSRGRGRG RGDGFYQRSF DDVEGGFGRG GREMHRSQSW EERGDRRFEK PGRKDPDGAP
AHFPLNHIRA NYEDPAAVNA RKFIHAECDN WRTTRDELNG EEDDNGWRLA GARRENERWC
PPSPDGPRSA GWREHPDQRR RFGFGDEERS GYRRPRSGSG SAEEERDSLP EWCLEDAEEE
TGTFDSSGAF LSLKVRALEK APKEPILEEA ELDFRPLEEN DEYAEKDDSE TEQTKDTDTN
TRHESDRNEE NCKSEEPSPV AVPFSAVVTP PKATTPAPIQ PVHLEKAEDK ERPSERTTLP
EIRHELSKAP LHTALSNSIV EAISIPHVAN KLPDLPVPAP SVLPVKSVPP QSQQVKPIEM
PVSVPPALLR STGSVGPISR PSALPSDLDE DEGLKHFEQE AEKMVAYLQD GGVDDERLAS
KIGPKPSALP ITNEAAFKWF YKDPQGEIQG PFNNQEMSEW FQAGYFTMTL QVKRGCDEMF
QPLGEMIKLW GRVPFTPGPT LPPILGDADQ ERMKRQQEIN ALNMYQLQQM QYQYLLRQQY
ALQQKVLNSA PPPPPPPQQQ LNLLLHQALK IRTPEPQQSL LPPVTRSMSV PDSGSVWEMQ
NPSTQASCSP NMQPAAPSTW EGSSVWDLPL DNMPQASSIE QLQLEKAKAL KLEMERREAE
LRAKREEEER KRLEEALRAR QEEERKRLEE EELARRKQEE ALKRQREQEE AQRRKKEEEE
RLAQEEALRR LEERRREEEE RRQREEFLRK QQEEERRKQE ELEAQRRREE EKRLEEEAAA
AAAALLRQQQ EEQKKREQEA QRQQELQRQR QQQQEALRRL QQQQQQQQLA QMKLPSSSKW
GQQSTTANSL SQSQNALSLA EIQKLEEERE RQTREEQRRQ QQELQRVQQQ QPQTKLPGWG
SVAKQPMATK SLLEIQREEA QQMKQRKDQQ QQQQQQQQPP PQPQPQQHST NTQQNRTQNR
AAINTSVWGS VNNVSSNWMM DSSVWGDTQN SNIGFWDEAV KEAAPPQTTR KSHTPKNKGN
ANLSNSSSGK ASKKVEEEEK LLKLFQGANK NQDGFMQWCE QTLHTLNTAN NLDVPTFASF
LKEVDSPYEV HDYVRAYLGD TPQAKDFAKQ FLERRAKQND NQQKPQQGQQ QKQQESVWGI
REVPQSVLLQ QQQQQQLQQQ QQQRFETVTS GKKKKKQKMV RADPSLLGFS VNASSERLNM
GEIETVEDF
