ID   GH31_ARATH              Reviewed;         590 AA.
AC   O82333;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Probable indole-3-acetic acid-amido synthetase GH3.1;
DE            EC=6.3.2.-;
DE   AltName: Full=Auxin-responsive GH3-like protein 1;
DE            Short=AtGH3-1;
GN   Name=GH3.1; OrderedLocusNames=At2g14960; ORFNames=T26I20.12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=15659623; DOI=10.1105/tpc.104.026690;
RA   Staswick P.E., Serban B., Rowe M., Tiryaki I., Maldonado M.T.,
RA   Maldonado M.C., Suza W.;
RT   "Characterization of an Arabidopsis enzyme family that conjugates amino
RT   acids to indole-3-acetic acid.";
RL   Plant Cell 17:616-627(2005).
RN   [4]
RP   NOMENCLATURE.
RX   PubMed=12036261; DOI=10.1023/a:1015207114117;
RA   Hagen G., Guilfoyle T.J.;
RT   "Auxin-responsive gene expression: genes, promoters and regulatory
RT   factors.";
RL   Plant Mol. Biol. 49:373-385(2002).
CC   -!- FUNCTION: Catalyzes the synthesis of indole-3-acetic acid (IAA)-amino
CC       acid conjugates, providing a mechanism for the plant to cope with the
CC       presence of excess auxin. {ECO:0000269|PubMed:15659623}.
CC   -!- INDUCTION: By auxin. {ECO:0000269|PubMed:15659623}.
CC   -!- SIMILARITY: Belongs to the IAA-amido conjugating enzyme family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC005396; AAC61292.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC06353.1; -; Genomic_DNA.
DR   PIR; D84523; D84523.
DR   RefSeq; NP_179101.1; NM_127059.3.
DR   AlphaFoldDB; O82333; -.
DR   SMR; O82333; -.
DR   STRING; 3702.AT2G14960.1; -.
DR   PaxDb; O82333; -.
DR   PRIDE; O82333; -.
DR   ProteomicsDB; 224791; -.
DR   EnsemblPlants; AT2G14960.1; AT2G14960.1; AT2G14960.
DR   GeneID; 815985; -.
DR   Gramene; AT2G14960.1; AT2G14960.1; AT2G14960.
DR   KEGG; ath:AT2G14960; -.
DR   Araport; AT2G14960; -.
DR   TAIR; locus:2060500; AT2G14960.
DR   eggNOG; ENOG502QPMW; Eukaryota.
DR   HOGENOM; CLU_016249_2_1_1; -.
DR   InParanoid; O82333; -.
DR   OMA; MNLCVPG; -.
DR   OrthoDB; 374623at2759; -.
DR   PhylomeDB; O82333; -.
DR   PRO; PR:O82333; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O82333; baseline and differential.
DR   Genevisible; O82333; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016881; F:acid-amino acid ligase activity; IBA:GO_Central.
DR   GO; GO:0009733; P:response to auxin; IMP:TAIR.
DR   InterPro; IPR004993; GH3.
DR   PANTHER; PTHR31901; PTHR31901; 1.
DR   Pfam; PF03321; GH3; 1.
PE   2: Evidence at transcript level;
KW   Ligase; Reference proteome.
FT   CHAIN           1..590
FT                   /note="Probable indole-3-acetic acid-amido synthetase
FT                   GH3.1"
FT                   /id="PRO_0000203570"
SQ   SEQUENCE   590 AA;  66734 MW;  648FE5D32E16BF6B CRC64;
     MAVDSNLSSP LGPPACEKDA KALRFIEEMT RNADTVQENL LAEILARNAD TEYLRRFNLC
     GATDRDTFKT KIPVITYEDL QPEIQRIADG DRSPILSAHP ISEFLTSSGT SAGERKLMPT
     IKEELDRRQL LYSLLMPVMN LYVPGLDKGK GMYFLFVKSE TKTPGGLPAR PVLTSYYKSE
     HFRSRPYDPY NVYTSPNEAI LCPDSFQSMY TQMLCGLLDR LSVLRVGAVF ASGLLRAIRF
     LQLHWSRFAH DIELGCLDSE ITDPSIRQCM SGILKPDPVL AEFIRRECKS DNWEKIITRI
     WPNTKYLDVI VTGAMAQYIP TLEYYSGGLP MACTMYASSE CYFGLNLNPM SKPSEVSYTI
     MPNMAYFEFI PLGGTKAVEL VDVNIGKEYE LVVTTYAGLC RYRVGDILRV TGFHNSAPQF
     HFVRRKNVLL SIDSDKTDES ELQKAVENAS SILHEECGSR VAEYTSYADT STIPGHYVLY
     WELLVRDGAR QPSHETLTRC CLGMEESLNS VYRQSRVADN SVGPLEIRVV RNGTFEELMD
     YAISRGASIN QYKVPRCVNF TPIVELLDSR VVSAHFSPSL PHWTPERRRR