ID   GH5FP_CHAOB             Reviewed;         556 AA.
AC   C0HLA0;
DT   20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT   20-JUN-2018, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Glycosyl hydrolase 5 family protein {ECO:0000303|PubMed:27237746};
DE            EC=3.2.1.- {ECO:0000305};
DE   AltName: Allergen=Cha o 3 {ECO:0000303|PubMed:27237746};
DE   Flags: Precursor;
OS   Chamaecyparis obtusa (Hinoki false-cypress) (Retinospora obtusa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers II; Cupressales; Cupressaceae;
OC   Chamaecyparis.
OX   NCBI_TaxID=13415 {ECO:0000303|PubMed:27237746};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-58 AND 514-532,
RP   FUNCTION, AND ALLERGEN.
RC   TISSUE=Flower {ECO:0000303|PubMed:27237746}, and
RC   Pollen {ECO:0000303|PubMed:27237746};
RX   PubMed=27237746; DOI=10.1016/j.jaci.2016.03.026;
RA   Osada T., Harada T., Asaka N., Haruma T., Kino K., Sasaki E., Okano M.,
RA   Yamada A., Utsugi T.;
RT   "Identification and gene cloning of a new major allergen Cha o 3 from
RT   Chamaecyparis obtusa (Japanese cypress) pollen.";
RL   J. Allergy Clin. Immunol. 138:911-913(2016).
RN   [2] {ECO:0000305}
RP   GLYCOSYLATION.
RC   TISSUE=Pollen {ECO:0000303|PubMed:28575723};
RX   PubMed=28575723; DOI=10.1016/j.carres.2017.05.005;
RA   Osada T., Maeda M., Tanabe C., Furuta K., Vavricka C.J., Sasaki E.,
RA   Okano M., Kimura Y.;
RT   "Glycoform of a newly identified pollen allergen, Cha o 3, from
RT   Chamaecyparis obtusa (Japanese cypress, Hinoki).";
RL   Carbohydr. Res. 448:18-23(2017).
CC   -!- FUNCTION: May have glycosyl hydrolase activity.
CC       {ECO:0000305|PubMed:27237746}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:28575723}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
CC       {ECO:0000269|PubMed:27237746}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
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DR   EMBL; HV942721; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; C0HLA0; -.
DR   SMR; C0HLA0; -.
DR   Allergome; 11866; Cha o 3.
DR   Allergome; 11867; Cha o 3.0101.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR035992; Ricin_B-like_lectins.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF50370; SSF50370; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   1: Evidence at protein level;
KW   Allergen; Carbohydrate metabolism; Direct protein sequencing; Glycoprotein;
KW   Glycosidase; Hydrolase; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000269|PubMed:27237746"
FT   CHAIN           29..556
FT                   /note="Glycosyl hydrolase 5 family protein"
FT                   /id="PRO_0000444568"
FT   ACT_SITE        208
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P07982"
FT   ACT_SITE        473
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:P07982"
FT   CARBOHYD        102
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        113
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        212
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        290
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        307
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        474
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        479
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   556 AA;  61637 MW;  C08352BFFF490ABB CRC64;
     MTSAGVAPTA LRLLTALLLL LVAAPSHSLP LLTRGRWIVD EATGLRVKLA CVNWVGHLEP
     GLPEGLNRLP VATVAHTISS LGFNCVRLTY SIHMLTRTSY TNATVAQTFA RLNLTEAASG
     IEHNNPELLD LGHVAAYHHV VAALSEAGVM VILDNHVSKP KWCCAVDDGN GFFGDRYFNP
     NTWVEGLGLM ATYFNNTPNV VAMSLRNELR GNRSTPISWS RHMQWGAATV HKANPKVLVI
     LSGLQFDTDL SFLPVLPVTL PFKEKIVYEG HWYSFGVPWR TGLPNDVCKN ETGRFKSNVG
     FVTSSANATA APLFMSEFGI DQRYVNDNDN RYLNCILAYL AEEDLDWALW TMGGSYYYRS
     DKQPVKDFEE TYGFFNHDWS RIRNPDFISR LKEIQQPIQD PYLAPGPYYQ IIYHPASGLC
     VESGIGNTVH LGSCQSVRSR WNYDASVKGP IGLMGSSSCI STQGNGLPAI MTENCSAPNN
     TLWSTVSSAQ LQLGTRVLGK DGKEKWMCLD GSKSPLISTN ECICITDSHC YPKLNPEKQW
     FKVITTNKQL LHQLQL