ID   GHC1_BOVIN              Reviewed;         322 AA.
AC   Q08DK4;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Mitochondrial glutamate carrier 1;
DE            Short=GC-1;
DE   AltName: Full=Glutamate/H(+) symporter 1;
DE   AltName: Full=Solute carrier family 25 member 22;
GN   Name=SLC25A22; Synonyms=GC1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Hippocampus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mitochondrial glutamate/H(+) symporter. Responsible for the
CC       transport of glutamate from the cytosol into the mitochondrial matrix
CC       with the concomitant import of a proton (By similarity). Plays a role
CC       in the control of glucose-stimulated insulin secretion (By similarity).
CC       {ECO:0000250|UniProtKB:A0A0G2K5L2, ECO:0000250|UniProtKB:Q9H936}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(in) + L-glutamate(in) = H(+)(out) + L-glutamate(out);
CC         Xref=Rhea:RHEA:70955, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985;
CC         Evidence={ECO:0000250|UniProtKB:Q9H936};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:A0A0G2K5L2}; Multi-pass membrane protein
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000305}.
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DR   EMBL; BC123701; AAI23702.1; -; mRNA.
DR   RefSeq; NP_001068627.1; NM_001075159.1.
DR   RefSeq; XP_005227358.1; XM_005227301.3.
DR   RefSeq; XP_005227359.1; XM_005227302.3.
DR   RefSeq; XP_005227360.1; XM_005227303.3.
DR   RefSeq; XP_015316820.1; XM_015461334.1.
DR   AlphaFoldDB; Q08DK4; -.
DR   SMR; Q08DK4; -.
DR   STRING; 9913.ENSBTAP00000001624; -.
DR   PaxDb; Q08DK4; -.
DR   PRIDE; Q08DK4; -.
DR   Ensembl; ENSBTAT00000001624; ENSBTAP00000001624; ENSBTAG00000001229.
DR   GeneID; 504371; -.
DR   KEGG; bta:504371; -.
DR   CTD; 79751; -.
DR   VEuPathDB; HostDB:ENSBTAG00000001229; -.
DR   VGNC; VGNC:34750; SLC25A22.
DR   eggNOG; KOG0750; Eukaryota.
DR   GeneTree; ENSGT00940000161196; -.
DR   HOGENOM; CLU_015166_3_4_1; -.
DR   InParanoid; Q08DK4; -.
DR   OMA; WQREGPT; -.
DR   OrthoDB; 945010at2759; -.
DR   TreeFam; TF313209; -.
DR   Reactome; R-BTA-428643; Organic anion transporters.
DR   Proteomes; UP000009136; Chromosome 29.
DR   Bgee; ENSBTAG00000001229; Expressed in retina and 103 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0005280; F:amino acid:proton symporter activity; ISS:UniProtKB.
DR   GO; GO:0015183; F:L-aspartate transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015810; P:aspartate transmembrane transport; IBA:GO_Central.
DR   GO; GO:0015813; P:L-glutamate transmembrane transport; IBA:GO_Central.
DR   GO; GO:0043490; P:malate-aspartate shuttle; IBA:GO_Central.
DR   GO; GO:0050796; P:regulation of insulin secretion; ISS:UniProtKB.
DR   Gene3D; 1.50.40.10; -; 1.
DR   InterPro; IPR002067; Mit_carrier.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   Pfam; PF00153; Mito_carr; 3.
DR   PRINTS; PR00926; MITOCARRIER.
DR   SUPFAM; SSF103506; SSF103506; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Repeat; Symport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..322
FT                   /note="Mitochondrial glutamate carrier 1"
FT                   /id="PRO_0000283051"
FT   TRANSMEM        12..32
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        62..82
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..243
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        292..312
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          6..93
FT                   /note="Solcar 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT   REPEAT          101..214
FT                   /note="Solcar 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT   REPEAT          223..312
FT                   /note="Solcar 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
SQ   SEQUENCE   322 AA;  34531 MW;  F322A3618958C337 CRC64;
     MADKQISLPA KLINGGIAGL IGVTCVFPID LAKTRLQNQQ NGQRMYTSMS DCLIKTIRSE
     GYFGMYRGAA VNLTLVTPEK AIKLAANDFF RYQLSKDGQQ LTLFKEMLAG CGAGTCQVIV
     TTPMEMLKIQ LQDAGRLAAQ RKILSAQAQL SGQGSAQPSV EAPATPRPTA TQLTRDLLRS
     RGIAGLYKGL GATLLRDVPF SIVYFPLFAN LNELGRPASG EKSPFYVSFL AGCVAGSAAA
     VAVNPCDVVK TRLQSLQRGI NEDTYSGFLD CARKILQNEG PSAFLKGAYC RALVIAPLFG
     IAQVVYFLGI AETLLGLPRV QP