ID   GHC1_PONAB              Reviewed;         323 AA.
AC   Q5RD81;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Mitochondrial glutamate carrier 1;
DE            Short=GC-1;
DE   AltName: Full=Glutamate/H(+) symporter 1;
DE   AltName: Full=Solute carrier family 25 member 22;
GN   Name=SLC25A22;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mitochondrial glutamate/H(+) symporter. Responsible for the
CC       transport of glutamate from the cytosol into the mitochondrial matrix
CC       with the concomitant import of a proton (By similarity). Plays a role
CC       in the control of glucose-stimulated insulin secretion (By similarity).
CC       {ECO:0000250|UniProtKB:A0A0G2K5L2, ECO:0000250|UniProtKB:Q9H936}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(in) + L-glutamate(in) = H(+)(out) + L-glutamate(out);
CC         Xref=Rhea:RHEA:70955, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985;
CC         Evidence={ECO:0000250|UniProtKB:Q9H936};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:A0A0G2K5L2}; Multi-pass membrane protein
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000305}.
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DR   EMBL; CR858035; CAH90276.1; -; mRNA.
DR   RefSeq; NP_001125123.1; NM_001131651.1.
DR   AlphaFoldDB; Q5RD81; -.
DR   SMR; Q5RD81; -.
DR   STRING; 9601.ENSPPYP00000003354; -.
DR   GeneID; 100172007; -.
DR   KEGG; pon:100172007; -.
DR   CTD; 79751; -.
DR   eggNOG; KOG0750; Eukaryota.
DR   InParanoid; Q5RD81; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0005280; F:amino acid:proton symporter activity; ISS:UniProtKB.
DR   GO; GO:0050796; P:regulation of insulin secretion; ISS:UniProtKB.
DR   Gene3D; 1.50.40.10; -; 1.
DR   InterPro; IPR002067; Mit_carrier.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   Pfam; PF00153; Mito_carr; 3.
DR   PRINTS; PR00926; MITOCARRIER.
DR   SUPFAM; SSF103506; SSF103506; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Repeat; Symport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..323
FT                   /note="Mitochondrial glutamate carrier 1"
FT                   /id="PRO_0000231653"
FT   TRANSMEM        12..32
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        62..82
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..243
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        292..312
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          6..93
FT                   /note="Solcar 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT   REPEAT          101..214
FT                   /note="Solcar 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT   REPEAT          223..312
FT                   /note="Solcar 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
SQ   SEQUENCE   323 AA;  34494 MW;  A0E6590AB6002793 CRC64;
     MADKQISLPA KLINGGIAGL IGVTCVFPID LAKTRLQNQQ NGQRMYTSMS DCLIKTIRSE
     GYFGMYRGAA VNLTLVTPEK AIKLAANDFF RHQLSKDGQK LTLLKEMLAG CGAGTCQVIV
     TTPMEMLKIQ LQDAGRIAAQ RKILAAQGQL SAQGGAQPSV EAPAAPRPTA IQLTRDLLRS
     RGIAGLYKGL GATLLRDVPL SVVYFPLFAN LNQLGRPASE EKSPFYVSFL AGCVAGSAAA
     VAVNPCDVVK TRLQSLQRGV NEDTYSGILD CARKILRHEG PSAFLKGAYC RALVIAPLFG
     IAQVVYFLGI AESLLGLLQD PQA