GHC1_RAT
ID GHC1_RAT Reviewed; 323 AA.
AC A0A0G2K5L2; D3ZRF5; Q5FVG4;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2015, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=Mitochondrial glutamate carrier 1;
DE Short=GC-1;
DE AltName: Full=Glutamate/H(+) symporter 1;
DE AltName: Full=Solute carrier family 25 member 22;
GN Name=Slc25a22 {ECO:0000312|RGD:1307826};
GN Synonyms=Gc1 {ECO:0000303|PubMed:19584051};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Li P.W., Adams M.D., Amanatides P.G., Baden-Tillson H.,
RA Barnstead M., Chin S.H., Dew I., Evans C.A., Ferriera S., Flanigan M.,
RA Fosler C., Glodek A., Gu Z., Holt R.A., Jennings D., Kraft C.L., Lu F.,
RA Nguyen T., Nusskern D.R., Pfannkoch C.M., Sitter C., Sutton G.G.,
RA Venter J.C., Wang Z., Woodage T., Zheng X.H., Zhong F.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION, AND TRANSPORTER
RP ACTIVITY.
RX PubMed=19584051; DOI=10.1074/jbc.m109.015495;
RA Casimir M., Lasorsa F.M., Rubi B., Caille D., Palmieri F., Meda P.,
RA Maechler P.;
RT "Mitochondrial glutamate carrier GC1 as a newly identified player in the
RT control of glucose-stimulated insulin secretion.";
RL J. Biol. Chem. 284:25004-25014(2009).
CC -!- FUNCTION: Mitochondrial glutamate/H(+) symporter (By similarity).
CC Responsible for the transport of glutamate from the cytosol into the
CC mitochondrial matrix with the concomitant import of a proton
CC (Probable). Plays a role in the control of glucose-stimulated insulin
CC secretion (PubMed:19584051). {ECO:0000250|UniProtKB:Q9H936,
CC ECO:0000269|PubMed:19584051, ECO:0000305|PubMed:19584051}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + L-glutamate(in) = H(+)(out) + L-glutamate(out);
CC Xref=Rhea:RHEA:70955, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985;
CC Evidence={ECO:0000305|PubMed:19584051};
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:19584051}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A0A0G2K5L2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A0A0G2K5L2-2; Sequence=VSP_061529;
CC -!- TISSUE SPECIFICITY: Detected in insulin-secreting beta-cells and
CC pancreatic islets (at the protein level).
CC {ECO:0000269|PubMed:19584051}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AABR07006025; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC109542; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH473953; EDM12050.1; -; Genomic_DNA.
DR EMBL; CH473953; EDM12051.1; -; Genomic_DNA.
DR EMBL; CH473953; EDM12052.1; -; Genomic_DNA.
DR EMBL; BC090010; AAH90010.1; -; mRNA.
DR RefSeq; NP_001014049.1; NM_001014027.1.
DR RefSeq; XP_008772957.1; XM_008774735.2.
DR RefSeq; XP_008772958.1; XM_008774736.2.
DR RefSeq; XP_008772959.1; XM_008774737.2.
DR RefSeq; XP_008772960.1; XM_008774738.2.
DR RefSeq; XP_017444722.1; XM_017589233.1.
DR RefSeq; XP_017445741.1; XM_017590252.1.
DR SMR; A0A0G2K5L2; -.
DR IntAct; Q5FVG4; 1.
DR Ensembl; ENSRNOT00000086238.2; ENSRNOP00000073467.1; ENSRNOG00000049944.3.
DR GeneID; 309111; -.
DR KEGG; rno:309111; -.
DR UCSC; RGD:1307826; rat.
DR CTD; 79751; -.
DR RGD; 1307826; Slc25a22.
DR eggNOG; KOG0750; Eukaryota.
DR GeneTree; ENSGT00940000161196; -.
DR HOGENOM; CLU_015166_3_4_1; -.
DR OMA; CTKKIWQ; -.
DR OrthoDB; 945010at2759; -.
DR TreeFam; TF313209; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Proteomes; UP000234681; Chromosome 1.
DR Bgee; ENSRNOG00000018450; Expressed in liver and 18 other tissues.
DR ExpressionAtlas; A0A0G2K5L2; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:UniProtKB.
DR GO; GO:0005280; F:amino acid:proton symporter activity; IEA:Ensembl.
DR GO; GO:0015183; F:L-aspartate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015810; P:aspartate transmembrane transport; IBA:GO_Central.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; IMP:UniProtKB.
DR GO; GO:0043490; P:malate-aspartate shuttle; IBA:GO_Central.
DR GO; GO:0050796; P:regulation of insulin secretion; IMP:UniProtKB.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..323
FT /note="Mitochondrial glutamate carrier 1"
FT /id="PRO_0000455802"
FT TRANSMEM 12..32
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 6..93
FT /note="Solcar 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 101..214
FT /note="Solcar 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 223..312
FT /note="Solcar 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT VAR_SEQ 138..231
FT /note="Missing (in isoform 2)"
FT /id="VSP_061529"
SQ SEQUENCE 323 AA; 34662 MW; A052DE7B70216744 CRC64;
MADKQISLPA KLINGGIAGL IGVTCVFPID LAKTRLQNQQ NGQRMYASMS DCLIKTIRSE
GYFGMYRGAA VNLTLVTPEK AIKLAANDFF RHQLSKDGQK LTLPKEMLAG CGAGTCQVIV
TTPMEMLKIQ LQDAGRIAAQ RKMLAAQAQL ATQGGGQPSV EAPAAPRPTA TQLTRDLLRN
HGIAGLYKGL GATLLRDVPF SIVYFPLFAN LNQLGRPSSE EKSPFYVSFL AGCVAGSAAA
VAVNPCDVVK TRLQSLERGV NEDTYSGFLD CARKIWRHEG PSAFLKGAYC RALVIAPLFG
IAQVVYFLGI AESLLGLLQE PQA
