GHC2_RAT
ID GHC2_RAT Reviewed; 320 AA.
AC Q505J6;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Mitochondrial glutamate carrier 2;
DE Short=GC-2;
DE AltName: Full=Glutamate/H(+) symporter 2;
DE AltName: Full=Solute carrier family 25 member 18;
GN Name=Slc25a18;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=19584051; DOI=10.1074/jbc.m109.015495;
RA Casimir M., Lasorsa F.M., Rubi B., Caille D., Palmieri F., Meda P.,
RA Maechler P.;
RT "Mitochondrial glutamate carrier GC1 as a newly identified player in the
RT control of glucose-stimulated insulin secretion.";
RL J. Biol. Chem. 284:25004-25014(2009).
CC -!- FUNCTION: Responsible for the transport of glutamate from the cytosol
CC into the mitochondrial matrix with the concomitant import of a proton
CC (symport system). {ECO:0000250|UniProtKB:Q9H1K4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + L-glutamate(in) = H(+)(out) + L-glutamate(out);
CC Xref=Rhea:RHEA:70955, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985;
CC Evidence={ECO:0000250|UniProtKB:Q9H1K4};
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:19584051}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH94517.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BC094517; AAH94517.1; ALT_INIT; mRNA.
DR RefSeq; NP_001037745.2; NM_001044280.2.
DR AlphaFoldDB; Q505J6; -.
DR SMR; Q505J6; -.
DR BioGRID; 596820; 1.
DR IntAct; Q505J6; 1.
DR MINT; Q505J6; -.
DR STRING; 10116.ENSRNOP00000015561; -.
DR iPTMnet; Q505J6; -.
DR PhosphoSitePlus; Q505J6; -.
DR jPOST; Q505J6; -.
DR PaxDb; Q505J6; -.
DR PRIDE; Q505J6; -.
DR Ensembl; ENSRNOT00000015560; ENSRNOP00000015561; ENSRNOG00000042731.
DR GeneID; 681896; -.
DR KEGG; rno:681896; -.
DR UCSC; RGD:1590470; rat.
DR CTD; 83733; -.
DR RGD; 1590470; Slc25a18.
DR eggNOG; KOG0750; Eukaryota.
DR GeneTree; ENSGT00940000162050; -.
DR HOGENOM; CLU_015166_3_4_1; -.
DR InParanoid; Q505J6; -.
DR OMA; LMTQVHS; -.
DR OrthoDB; 945010at2759; -.
DR PhylomeDB; Q505J6; -.
DR TreeFam; TF313209; -.
DR Reactome; R-RNO-428643; Organic anion transporters.
DR PRO; PR:Q505J6; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000042731; Expressed in cerebellum and 6 other tissues.
DR Genevisible; Q505J6; RN.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:UniProtKB.
DR GO; GO:0005280; F:amino acid:proton symporter activity; ISS:UniProtKB.
DR GO; GO:0015183; F:L-aspartate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015810; P:aspartate transmembrane transport; IBA:GO_Central.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; ISS:UniProtKB.
DR GO; GO:0043490; P:malate-aspartate shuttle; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein;
KW Reference proteome; Repeat; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..320
FT /note="Mitochondrial glutamate carrier 2"
FT /id="PRO_0000090623"
FT TRANSMEM 17..37
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 11..97
FT /note="Solcar 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 105..215
FT /note="Solcar 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 224..313
FT /note="Solcar 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT MOD_RES 150
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H1K4"
SQ SEQUENCE 320 AA; 34170 MW; 658DCFBF6B5AB51C CRC64;
MIACRMSSQD LSITAKLING GIAGLVGVTC VFPIDLAKTR LQNQQGKDVY KGMTDCLVKT
ARAEGFLGMY RGAAVNLTLV TPEKAIKLAA NDFLRQLLMQ DGTQRNLKME MLAGCGAGIC
QVVITCPMEM LKIQLQDAGR LAVCQQASAS ATPTSRPYST GSTSTHRRPS ATLIAWELLR
TQGLSGLYRG LGATLLRDIP FSIIYFPLFA NLNQLGVSEL TGKASFTHSF VAGCAAGSVS
AVAVTPLDVL KTRIQTLKKG LGEDTYRGVT DCARKLWTQE GAAAFMKGAG CRALVIAPLF
GIAQGVYFIG IGERILKCFE
