ID   GHOS_ECO57              Reviewed;          98 AA.
AC   P0AF62; P39275;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Endoribonuclease antitoxin GhoS {ECO:0000305};
DE            EC=3.1.-.-;
DE   AltName: Full=Antitoxin GhoS;
GN   Name=ghoS {ECO:0000305}; OrderedLocusNames=Z5730, ECs5110;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Antitoxin component of a type V toxin-antitoxin (TA) system.
CC       Neutralizes the toxic effects of toxin GhoT by digesting ghoT
CC       transcripts in a sequence-specific manner. In concert with GhoT is
CC       involved in reducing cell growth during antibacterial stress.
CC       {ECO:0000250|UniProtKB:P0AF61}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P0AF61}.
CC   -!- PTM: Unlike other TA antitoxins, this protein is stable.
CC       {ECO:0000250|UniProtKB:P0AF61}.
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DR   EMBL; AE005174; AAG59327.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB38533.1; -; Genomic_DNA.
DR   PIR; C86108; C86108.
DR   PIR; F91267; F91267.
DR   RefSeq; NP_313137.1; NC_002695.1.
DR   RefSeq; WP_000398619.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AF62; -.
DR   BMRB; P0AF62; -.
DR   SMR; P0AF62; -.
DR   STRING; 155864.EDL933_5475; -.
DR   EnsemblBacteria; AAG59327; AAG59327; Z5730.
DR   EnsemblBacteria; BAB38533; BAB38533; ECs_5110.
DR   GeneID; 66671961; -.
DR   GeneID; 914192; -.
DR   KEGG; ece:Z5730; -.
DR   KEGG; ecs:ECs_5110; -.
DR   PATRIC; fig|386585.9.peg.5340; -.
DR   HOGENOM; CLU_182150_0_0_6; -.
DR   OMA; DDYFRQI; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   Gene3D; 3.30.70.2360; -; 1.
DR   InterPro; IPR022597; GhoS.
DR   InterPro; IPR038241; GhoS_sf.
DR   Pfam; PF11080; GhoS; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Toxin-antitoxin system; Transcription; Transcription regulation.
FT   CHAIN           1..98
FT                   /note="Endoribonuclease antitoxin GhoS"
FT                   /id="PRO_0000169734"
SQ   SEQUENCE   98 AA;  11468 MW;  8466741C8225E468 CRC64;
     MEGKNKFNTY VVSFDYPSSY SSVFLRLRSL MYDMNFSSIV ADEYGIPRQL NENSFAITTS
     LAASEIEDLI RLKCLDLPDI DFDLNIMTVD DYFRQFYK