ID   GHOT_ECO57              Reviewed;          57 AA.
AC   P64648; P58038;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Toxin GhoT {ECO:0000305};
GN   Name=ghoT {ECO:0000305}; OrderedLocusNames=Z5731, ECs5110.1;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Toxic component of a type V toxin-antitoxin (TA) system.
CC       Causes membrane damage when induced by MqsR, slowing cell growth and
CC       leading to the formation of dormant persister cells; involved with
CC       GhoS, its antitoxin, in reducing cell growth during antibacterial
CC       stress. Its toxic effects are neutralized by GhoS, which digests ghoT
CC       transcripts in a sequence-specific manner.
CC       {ECO:0000250|UniProtKB:P64646}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P64646}; Multi-pass membrane protein
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GhoT/OrtT toxin family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG59328.1; -; Genomic_DNA.
DR   EMBL; BA000007; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; D86108; D86108.
DR   RefSeq; WP_001173343.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P64648; -.
DR   STRING; 155864.EDL933_5476; -.
DR   EnsemblBacteria; AAG59328; AAG59328; Z5731.
DR   GeneID; 66671960; -.
DR   KEGG; ece:Z5731; -.
DR   PATRIC; fig|83334.175.peg.5666; -.
DR   OMA; ITWYLAR; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019689; Toxin_GhoT/OrtT.
DR   Pfam; PF10753; Toxin_GhoT_OrtT; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT   CHAIN           1..57
FT                   /note="Toxin GhoT"
FT                   /id="PRO_0000169736"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   57 AA;  6555 MW;  A3670A19500F75D6 CRC64;
     MALFSKILIF YVIGVNISFV IIWFISHEKT HIRLLSAFLV GITWPMSLPV ALLFSLF