ID   GHRP_LITCT              Reviewed;         221 AA.
AC   Q8JIM3; P83339;
DT   06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Growth hormone-releasing peptides;
DE   Contains:
DE     RecName: Full=Growth hormone-releasing peptide;
DE              Short=GH-releasing peptide;
DE     AltName: Full=fGRP;
DE   Contains:
DE     RecName: Full=fGRP-related peptide 1;
DE   Contains:
DE     RecName: Full=fGRP-related peptide 2;
DE   Contains:
DE     RecName: Full=fGRP-related peptide 3;
DE   Flags: Precursor;
OS   Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8400;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND AMIDATION AT PHE-107; PHE-136; PHE-156 AND
RP   PHE-176.
RC   TISSUE=Brain;
RX   PubMed=12208659; DOI=10.1677/joe.0.1740395;
RA   Sawada K., Ukena K., Kikuyama S., Tsutsui K.;
RT   "Identification of a cDNA encoding a novel amphibian growth hormone-
RT   releasing peptide and localization of its transcript.";
RL   J. Endocrinol. 174:395-402(2002).
RN   [2]
RP   PROTEIN SEQUENCE OF 96-107, AMIDATION AT PHE-107, SYNTHESIS, FUNCTION,
RP   TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Hypothalamus;
RX   PubMed=11796493; DOI=10.1210/endo.143.2.8630;
RA   Koda A., Ukena K., Teranishi H., Ohta S., Yamamoto K., Kikuyama S.,
RA   Tsutsui K.;
RT   "A novel amphibian hypothalamic neuropeptide: isolation, localization, and
RT   biological activity.";
RL   Endocrinology 143:411-419(2002).
CC   -!- FUNCTION: Primary role is to release GH from the pituitary. May act as
CC       an endogenous ligand in the bullfrog hypothalamo-hypophysial system.
CC       {ECO:0000269|PubMed:11796493}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Observed in the suprachiasmatic nucleus and in
CC       several telencephalic and diencephalic regions.
CC       {ECO:0000269|PubMed:11796493}.
CC   -!- MASS SPECTROMETRY: [Growth hormone-releasing peptide]: Mass=1326.5;
CC       Method=FAB; Evidence={ECO:0000269|PubMed:11796493};
CC   -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC       {ECO:0000305}.
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DR   EMBL; AB080743; BAC11854.1; -; mRNA.
DR   AlphaFoldDB; Q8JIM3; -.
DR   PRIDE; Q8JIM3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046879; P:hormone secretion; IDA:UniProtKB.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; TAS:UniProtKB.
DR   InterPro; IPR002544; FMRFamid-related_peptide-like.
DR   InterPro; IPR026297; FMRFamide-related/fGRP.
DR   PANTHER; PTHR14403; PTHR14403; 1.
DR   Pfam; PF01581; FARP; 2.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Neuropeptide; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   PROPEP          26..95
FT                   /evidence="ECO:0000269|PubMed:11796493"
FT                   /id="PRO_0000009937"
FT   PEPTIDE         96..107
FT                   /note="Growth hormone-releasing peptide"
FT                   /id="PRO_0000009938"
FT   PROPEP          110..127
FT                   /id="PRO_0000009939"
FT   PEPTIDE         128..136
FT                   /note="fGRP-related peptide 1"
FT                   /id="PRO_0000009940"
FT   PEPTIDE         139..156
FT                   /note="fGRP-related peptide 2"
FT                   /id="PRO_0000009941"
FT   PEPTIDE         159..176
FT                   /note="fGRP-related peptide 3"
FT                   /id="PRO_0000009942"
FT   PROPEP          179..221
FT                   /id="PRO_0000009943"
FT   MOD_RES         107
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:11796493,
FT                   ECO:0000269|PubMed:12208659"
FT   MOD_RES         136
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:12208659"
FT   MOD_RES         156
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:12208659"
FT   MOD_RES         176
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:12208659"
SQ   SEQUENCE   221 AA;  25886 MW;  E0DFEC1CBBB1CF00 CRC64;
     MHLKIGTLTI RTIMLFTLCT FLTLFAFSTC FDETKTINLE SQEIYDDLFE SKEDLQNERN
     GNSDEYRYMG SNSINDINRY NRLSMQPENE FLQERSLKPA ANLPLRFGRT SDDKIAKSIP
     SFDKIAKSIP NLPQRFGRYL SGKTKVQSMA NLPQRFGRAQ YTNHFVHSLD TLPLRFGRTP
     HSDRLQYEMN SHPLELKNPE EDSDRKKRQA MTFRIRTDLQ M